Zeaxanthin radical cation formation in minor light-harvesting complexes of higher plant antenna
Avenson, T. J. ; Ahn, T. K. ; Zigmantas, Donatas LU
; Niyogi, K. K.
; Li, Z.
; Ballottari, M.
; Bassi, R.
and Fleming, G. R.
(2008)
In Journal of Biological Chemistry
283(6).
p.3550-3558
- Abstract
- Previous work on intact thylakoid membranes showed that transient formation of a zeaxanthin radical cation was correlated with regulation of photosynthetic light-harvesting via energy-dependent quenching. A molecular mechanism for such quenching was proposed to involve charge transfer within a chlorophyll-zeaxanthin heterodimer. Using near infrared (880 - 1100 nm) transient absorption spectroscopy, we demonstrate that carotenoid (mainly zeaxanthin) radical cation generation occurs solely in isolated minor light-harvesting complexes that bind zeaxanthin, consistent with the engagement of charge transfer quenching therein. We estimated that less than 0.5% of the isolated minor complexes undergo charge transfer quenching in vitro, whereas the... (More)
- Previous work on intact thylakoid membranes showed that transient formation of a zeaxanthin radical cation was correlated with regulation of photosynthetic light-harvesting via energy-dependent quenching. A molecular mechanism for such quenching was proposed to involve charge transfer within a chlorophyll-zeaxanthin heterodimer. Using near infrared (880 - 1100 nm) transient absorption spectroscopy, we demonstrate that carotenoid (mainly zeaxanthin) radical cation generation occurs solely in isolated minor light-harvesting complexes that bind zeaxanthin, consistent with the engagement of charge transfer quenching therein. We estimated that less than 0.5% of the isolated minor complexes undergo charge transfer quenching in vitro, whereas the fraction of minor complexes estimated to be engaged in charge transfer quenching in isolated thylakoids was more than 80 times higher. We conclude that minor complexes which bind zeaxanthin are sites of charge transfer quenching in vivo and that they can assume Non-quenching and Quenching conformations, the equilibrium LHC(N) reversible arrow LHC(Q) of which is modulated by the transthylakoid pH gradient, the PsbS protein, and protein-protein interactions. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1432904
- author
- Avenson, T. J.
; Ahn, T. K.
; Zigmantas, Donatas
LU
; Niyogi, K. K.
; Li, Z.
; Ballottari, M.
; Bassi, R.
and Fleming, G. R.
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- xanthophyll cycle, chlorophyll fluorescence, resolved fluorescence analysis, photosystem-ii, energy-dissipation, excited-states, in-vivo, protein cp26, oxygenic photosynthesis, green plants
- in
- Journal of Biological Chemistry
- volume
- 283
- issue
- 6
- pages
- 3550 - 3558
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- scopus:41249094699
- ISSN
- 1083-351X
- DOI
- 10.1074/jbc.M705645200
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Chemical Physics (S) (011001060)
- id
- ee2e5b7c-ad72-4087-8abe-c43b34c40388 (old id 1432904)
- date added to LUP
- 2016-04-04 08:56:32
- date last changed
- 2022-01-29 07:55:51
@article{ee2e5b7c-ad72-4087-8abe-c43b34c40388,
abstract = {{Previous work on intact thylakoid membranes showed that transient formation of a zeaxanthin radical cation was correlated with regulation of photosynthetic light-harvesting via energy-dependent quenching. A molecular mechanism for such quenching was proposed to involve charge transfer within a chlorophyll-zeaxanthin heterodimer. Using near infrared (880 - 1100 nm) transient absorption spectroscopy, we demonstrate that carotenoid (mainly zeaxanthin) radical cation generation occurs solely in isolated minor light-harvesting complexes that bind zeaxanthin, consistent with the engagement of charge transfer quenching therein. We estimated that less than 0.5% of the isolated minor complexes undergo charge transfer quenching in vitro, whereas the fraction of minor complexes estimated to be engaged in charge transfer quenching in isolated thylakoids was more than 80 times higher. We conclude that minor complexes which bind zeaxanthin are sites of charge transfer quenching in vivo and that they can assume Non-quenching and Quenching conformations, the equilibrium LHC(N) reversible arrow LHC(Q) of which is modulated by the transthylakoid pH gradient, the PsbS protein, and protein-protein interactions.}},
author = {{Avenson, T. J. and Ahn, T. K. and Zigmantas, Donatas and Niyogi, K. K. and Li, Z. and Ballottari, M. and Bassi, R. and Fleming, G. R.}},
issn = {{1083-351X}},
keywords = {{xanthophyll cycle; chlorophyll fluorescence; resolved fluorescence analysis; photosystem-ii; energy-dissipation; excited-states; in-vivo; protein cp26; oxygenic photosynthesis; green plants}},
language = {{eng}},
number = {{6}},
pages = {{3550--3558}},
publisher = {{American Society for Biochemistry and Molecular Biology}},
series = {{Journal of Biological Chemistry}},
title = {{Zeaxanthin radical cation formation in minor light-harvesting complexes of higher plant antenna}},
url = {{http://dx.doi.org/10.1074/jbc.M705645200}},
doi = {{10.1074/jbc.M705645200}},
volume = {{283}},
year = {{2008}},
}