Changes in Membrane Phospholipids as a Mechanistic Explanation for Decreased Freeze Tolerance in Earthworms Exposed to Sublethal Copper Concentrations
Bindesbol, Anne-Mette ; Bayley, Mark ; Damgaard, Christian ; Hedlund, Katarina LU
and Holmstrup, Martin
(2009)
In Environmental Science & Technology
43(14).
p.5495-5500
- Abstract
- At low temperature, cell membrane functionality depends on adjustments of membrane phospholipid fatty acid (PLFA) composition. We here test the hypothesis that previous exposure to copper (Cu) may deteriorate tolerance to freezing temperatures because of Cu-induced changes of PLFA composition of cell membranes in the freeze-tolerant earthworm Dendrobaena octaedra. Cu levels and freezing temperatures were varied in a full factorial design. We measured PLFA composition and lipid peroxidation. A highly significant interaction was observed between subzero temperatures and Cu concentrations above 120 mg/kg dry soil. Lipid peroxidation increased slightly in worms exposed to Cu. In particular, the analysis showed that Cu had a significant... (More)
- At low temperature, cell membrane functionality depends on adjustments of membrane phospholipid fatty acid (PLFA) composition. We here test the hypothesis that previous exposure to copper (Cu) may deteriorate tolerance to freezing temperatures because of Cu-induced changes of PLFA composition of cell membranes in the freeze-tolerant earthworm Dendrobaena octaedra. Cu levels and freezing temperatures were varied in a full factorial design. We measured PLFA composition and lipid peroxidation. A highly significant interaction was observed between subzero temperatures and Cu concentrations above 120 mg/kg dry soil. Lipid peroxidation increased slightly in worms exposed to Cu. In particular, the analysis showed that Cu had a significant negative effect on the polyunsaturated PLFA, linoleic acid (18:2 omega 6,9), which has previously been reported to correlate positively (R-2 = 0.92) with freeze tolerance in D. octaedra. This supports our hypothesis that reduced tolerance to freezing temperatures in Cu-exposed worms may be due to membrane damage. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1461698
- author
- Bindesbol, Anne-Mette
; Bayley, Mark
; Damgaard, Christian
; Hedlund, Katarina
LU
and Holmstrup, Martin
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Environmental Science & Technology
- volume
- 43
- issue
- 14
- pages
- 5495 - 5500
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000268138000054
- scopus:67650483694
- ISSN
- 1520-5851
- DOI
- 10.1021/es900898y
- language
- English
- LU publication?
- yes
- id
- d127567d-2071-4748-b996-09807a0c4a66 (old id 1461698)
- date added to LUP
- 2016-04-01 12:52:05
- date last changed
- 2022-01-27 08:01:35
@article{d127567d-2071-4748-b996-09807a0c4a66,
abstract = {{At low temperature, cell membrane functionality depends on adjustments of membrane phospholipid fatty acid (PLFA) composition. We here test the hypothesis that previous exposure to copper (Cu) may deteriorate tolerance to freezing temperatures because of Cu-induced changes of PLFA composition of cell membranes in the freeze-tolerant earthworm Dendrobaena octaedra. Cu levels and freezing temperatures were varied in a full factorial design. We measured PLFA composition and lipid peroxidation. A highly significant interaction was observed between subzero temperatures and Cu concentrations above 120 mg/kg dry soil. Lipid peroxidation increased slightly in worms exposed to Cu. In particular, the analysis showed that Cu had a significant negative effect on the polyunsaturated PLFA, linoleic acid (18:2 omega 6,9), which has previously been reported to correlate positively (R-2 = 0.92) with freeze tolerance in D. octaedra. This supports our hypothesis that reduced tolerance to freezing temperatures in Cu-exposed worms may be due to membrane damage.}},
author = {{Bindesbol, Anne-Mette and Bayley, Mark and Damgaard, Christian and Hedlund, Katarina and Holmstrup, Martin}},
issn = {{1520-5851}},
language = {{eng}},
number = {{14}},
pages = {{5495--5500}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Environmental Science & Technology}},
title = {{Changes in Membrane Phospholipids as a Mechanistic Explanation for Decreased Freeze Tolerance in Earthworms Exposed to Sublethal Copper Concentrations}},
url = {{http://dx.doi.org/10.1021/es900898y}},
doi = {{10.1021/es900898y}},
volume = {{43}},
year = {{2009}},
}