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Genes regulating molecular and cellular functions in noninfectious nonallergic rhinitis

Cardell, L. O. ; Andersson, Morgan LU ; Cervin, Anders LU ; Davidsson, A. ; Hellgren, J. ; Holmstrom, M. ; Lundblad, L. ; Stierna, P. ; Stjarne, P. and Adner, M. (2009) In Allergy 64(9). p.1301-1308
Abstract
Background: Chronic noninfectious, nonallergic rhinitis (NINAR) is a complex syndrome with a principally unknown pathophysiology. New technology has made it possible to examine differentially expressed genes and according to network theory, genes connected by their function that might have key roles in the disease. Methods: Connectivity analysis was used to identify NINAR key genes. mRNA was extracted from nasal biopsies from 12 NINAR patients and 12 healthy volunteers. Microarrays were performed using Affymetrix chips with 54 613 genes. Data were analysed with the Ingenuity Pathway System for organization of genes into annotated biological functions and, thereafter, linking genes into networks due to their connectivity. The regulation of... (More)
Background: Chronic noninfectious, nonallergic rhinitis (NINAR) is a complex syndrome with a principally unknown pathophysiology. New technology has made it possible to examine differentially expressed genes and according to network theory, genes connected by their function that might have key roles in the disease. Methods: Connectivity analysis was used to identify NINAR key genes. mRNA was extracted from nasal biopsies from 12 NINAR patients and 12 healthy volunteers. Microarrays were performed using Affymetrix chips with 54 613 genes. Data were analysed with the Ingenuity Pathway System for organization of genes into annotated biological functions and, thereafter, linking genes into networks due to their connectivity. The regulation of key genes was confirmed with reverse transcription-polymerase chain reaction (RT-PCR). Results: In all, 43 genes were differentially expressed. The functional analysis showed that these genes were primarily involved in cellular movement, haematological system development and immune response. Merging these functions, 10 genes were found to be shared. Network analysis generated three networks and two of these 'shared genes' in key positions, c-fos and cell division cycle 42 (Cdc42). These genes were upregulated in both the array and the RT-PCR analysis. Conclusion: Ten genes were found to be of pathophysiological interest for NINAR and of these, c-fos and Cdc42 seemed to be of specific interest due to their ability to interact with other genes of interest within this context. Although the role of c-fos and Cdc42 in upper airway inflammation remains unknown, they might be used as potential disease markers. (Less)
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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ingenuity Pathway, connectivity analysis, cell division cycle 42, c-fos, System, microarray
in
Allergy
volume
64
issue
9
pages
1301 - 1308
publisher
Wiley-Blackwell
external identifiers
  • wos:000268968700008
  • scopus:68949209326
  • pmid:19432938
ISSN
1398-9995
DOI
10.1111/j.1398-9995.2009.02009.x
language
English
LU publication?
yes
id
388d5941-51df-4518-ae30-ed21288a5743 (old id 1477486)
date added to LUP
2016-04-01 13:54:28
date last changed
2022-01-27 21:49:14
@article{388d5941-51df-4518-ae30-ed21288a5743,
  abstract     = {{Background: Chronic noninfectious, nonallergic rhinitis (NINAR) is a complex syndrome with a principally unknown pathophysiology. New technology has made it possible to examine differentially expressed genes and according to network theory, genes connected by their function that might have key roles in the disease. Methods: Connectivity analysis was used to identify NINAR key genes. mRNA was extracted from nasal biopsies from 12 NINAR patients and 12 healthy volunteers. Microarrays were performed using Affymetrix chips with 54 613 genes. Data were analysed with the Ingenuity Pathway System for organization of genes into annotated biological functions and, thereafter, linking genes into networks due to their connectivity. The regulation of key genes was confirmed with reverse transcription-polymerase chain reaction (RT-PCR). Results: In all, 43 genes were differentially expressed. The functional analysis showed that these genes were primarily involved in cellular movement, haematological system development and immune response. Merging these functions, 10 genes were found to be shared. Network analysis generated three networks and two of these 'shared genes' in key positions, c-fos and cell division cycle 42 (Cdc42). These genes were upregulated in both the array and the RT-PCR analysis. Conclusion: Ten genes were found to be of pathophysiological interest for NINAR and of these, c-fos and Cdc42 seemed to be of specific interest due to their ability to interact with other genes of interest within this context. Although the role of c-fos and Cdc42 in upper airway inflammation remains unknown, they might be used as potential disease markers.}},
  author       = {{Cardell, L. O. and Andersson, Morgan and Cervin, Anders and Davidsson, A. and Hellgren, J. and Holmstrom, M. and Lundblad, L. and Stierna, P. and Stjarne, P. and Adner, M.}},
  issn         = {{1398-9995}},
  keywords     = {{Ingenuity Pathway; connectivity analysis; cell division cycle 42; c-fos; System; microarray}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{1301--1308}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Allergy}},
  title        = {{Genes regulating molecular and cellular functions in noninfectious nonallergic rhinitis}},
  url          = {{http://dx.doi.org/10.1111/j.1398-9995.2009.02009.x}},
  doi          = {{10.1111/j.1398-9995.2009.02009.x}},
  volume       = {{64}},
  year         = {{2009}},
}