Characterization of graphite electrodes modified with laccases from Trametes hirsuta and Cerrena unicolor and their use for flow injection amperometric determination of some phenolic compounds
(2005) In International Journal of Environmental Analytical Chemistry 85(9-11). p.753-770- Abstract
- Fungal laccases from Trametes hirsuta and Cerrena unicolor were immobilized on spectroscopic graphite electrodes using physical absorption. The laccase-modified graphite electrodes were mounted as the working electrodes into a wall jet flow through an electrochemical cell and then used for flow injection amperometric determination of different phenolic compounds of relevance both for environmental and clinical analysis. At optimum conditions for determination of catechol serving as a model compound, the output response signals of the laccase-modified electrodes were recorded as the result of injections of 50 mu L of solutions with different phenolic Substrates into the carrier citrate buffer solution with a working potential of -50mV vs.... (More)
- Fungal laccases from Trametes hirsuta and Cerrena unicolor were immobilized on spectroscopic graphite electrodes using physical absorption. The laccase-modified graphite electrodes were mounted as the working electrodes into a wall jet flow through an electrochemical cell and then used for flow injection amperometric determination of different phenolic compounds of relevance both for environmental and clinical analysis. At optimum conditions for determination of catechol serving as a model compound, the output response signals of the laccase-modified electrodes were recorded as the result of injections of 50 mu L of solutions with different phenolic Substrates into the carrier citrate buffer solution with a working potential of -50mV vs. Ag vertical bar AgCl. Statistical aspects were applied and the Michaelis-Menten constants evaluated were correlated to the chemical structure or the investigated phenolic compounds. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/150942
- author
- Haghighi, Behzad LU ; Jarosz-Wilkolazka, A ; Ruzgas, Tautgirdas LU ; Gorton, Lo LU and Leonowicz, A
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Phenols, Laccase, Amperometry, Flow injection, Biosensors
- in
- International Journal of Environmental Analytical Chemistry
- volume
- 85
- issue
- 9-11
- pages
- 753 - 770
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000231982500016
- scopus:33644668847
- ISSN
- 1029-0397
- DOI
- 10.1080/03067310500153710
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 3efe1ca1-b1c0-4cfb-a200-91cb4963a6aa (old id 150942)
- date added to LUP
- 2016-04-01 12:04:23
- date last changed
- 2022-01-26 22:24:04
@article{3efe1ca1-b1c0-4cfb-a200-91cb4963a6aa,
abstract = {{Fungal laccases from Trametes hirsuta and Cerrena unicolor were immobilized on spectroscopic graphite electrodes using physical absorption. The laccase-modified graphite electrodes were mounted as the working electrodes into a wall jet flow through an electrochemical cell and then used for flow injection amperometric determination of different phenolic compounds of relevance both for environmental and clinical analysis. At optimum conditions for determination of catechol serving as a model compound, the output response signals of the laccase-modified electrodes were recorded as the result of injections of 50 mu L of solutions with different phenolic Substrates into the carrier citrate buffer solution with a working potential of -50mV vs. Ag vertical bar AgCl. Statistical aspects were applied and the Michaelis-Menten constants evaluated were correlated to the chemical structure or the investigated phenolic compounds.}},
author = {{Haghighi, Behzad and Jarosz-Wilkolazka, A and Ruzgas, Tautgirdas and Gorton, Lo and Leonowicz, A}},
issn = {{1029-0397}},
keywords = {{Phenols; Laccase; Amperometry; Flow injection; Biosensors}},
language = {{eng}},
number = {{9-11}},
pages = {{753--770}},
publisher = {{Taylor & Francis}},
series = {{International Journal of Environmental Analytical Chemistry}},
title = {{Characterization of graphite electrodes modified with laccases from Trametes hirsuta and Cerrena unicolor and their use for flow injection amperometric determination of some phenolic compounds}},
url = {{http://dx.doi.org/10.1080/03067310500153710}},
doi = {{10.1080/03067310500153710}},
volume = {{85}},
year = {{2005}},
}