Selective immuno-supported liquid membrane (ISLM) extraction, enrichment and analysis of 2,4,6-trichlorophenol
(2005) In Journal of Membrane Science 256(1-2). p.143-149- Abstract
- 2,4,6-Trichlorophenol (2,4,6-TCP) was extracted from liquid samples (standards and spiked river water) using immuno-supported liquid membrane (ISLM) extraction followed by fluorescence flow immunoassay (FFIA) detection of the immuno-extracted analyte. ISLM-FFIA involves simultaneous on-line sample clean-up, enrichment and detection of analytes. The analyte is transported from a continuously flowing aqueous sample phase (donor) through a supported liquid membrane (SLM), containing an immobilised organic solvent, to a stagnant aqueous phase (acceptor), containing excess anti-analyte antibody, where it is trapped as analyte-anti body complex. The acceptor content is then dispensed and mixed on-line with a fluorescent analyte tracer... (More)
- 2,4,6-Trichlorophenol (2,4,6-TCP) was extracted from liquid samples (standards and spiked river water) using immuno-supported liquid membrane (ISLM) extraction followed by fluorescence flow immunoassay (FFIA) detection of the immuno-extracted analyte. ISLM-FFIA involves simultaneous on-line sample clean-up, enrichment and detection of analytes. The analyte is transported from a continuously flowing aqueous sample phase (donor) through a supported liquid membrane (SLM), containing an immobilised organic solvent, to a stagnant aqueous phase (acceptor), containing excess anti-analyte antibody, where it is trapped as analyte-anti body complex. The acceptor content is then dispensed and mixed on-line with a fluorescent analyte tracer (2,4,6-TCP/NH/(CH2)(3)/CO/EDF) and the resulting labelled fraction, separated from the mixture by restricted access column, is an indirect measure of the analyte amount extracted in the acceptor. The performance of ISLM-FFIA was compared with a similar FFIA (without the extraction step) with the following results: the ISLM-FFIA configuration leads to improved assay sensitivity (LOD 27.92 +/- 2.44 and 224 +/- 15.86 mu g L-1 for ISLM-FFIA and FFIA, respectively) and improved selectivity and recovery for 2,4,6-TCP in presence of other chlorophenols (e.g. 2,4,5-trichlorophenol and 4-chlorophenol) compared to FFIA alone. (c) 2005 Elsevier B.V. All rights reserved. (Less)
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https://lup.lub.lu.se/record/151034
- author
- Tudorache, Madalina LU and Emnéus, Jenny LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Membrane Science
- volume
- 256
- issue
- 1-2
- pages
- 143 - 149
- publisher
- Elsevier
- external identifiers
-
- wos:000229616800017
- scopus:19544377702
- ISSN
- 0376-7388
- DOI
- 10.1016/j.memsci.2005.01.045
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 06d0913c-4f36-44f4-b336-55d91d539e28 (old id 151034)
- date added to LUP
- 2016-04-01 16:55:43
- date last changed
- 2022-01-28 23:06:39
@article{06d0913c-4f36-44f4-b336-55d91d539e28,
abstract = {{2,4,6-Trichlorophenol (2,4,6-TCP) was extracted from liquid samples (standards and spiked river water) using immuno-supported liquid membrane (ISLM) extraction followed by fluorescence flow immunoassay (FFIA) detection of the immuno-extracted analyte. ISLM-FFIA involves simultaneous on-line sample clean-up, enrichment and detection of analytes. The analyte is transported from a continuously flowing aqueous sample phase (donor) through a supported liquid membrane (SLM), containing an immobilised organic solvent, to a stagnant aqueous phase (acceptor), containing excess anti-analyte antibody, where it is trapped as analyte-anti body complex. The acceptor content is then dispensed and mixed on-line with a fluorescent analyte tracer (2,4,6-TCP/NH/(CH2)(3)/CO/EDF) and the resulting labelled fraction, separated from the mixture by restricted access column, is an indirect measure of the analyte amount extracted in the acceptor. The performance of ISLM-FFIA was compared with a similar FFIA (without the extraction step) with the following results: the ISLM-FFIA configuration leads to improved assay sensitivity (LOD 27.92 +/- 2.44 and 224 +/- 15.86 mu g L-1 for ISLM-FFIA and FFIA, respectively) and improved selectivity and recovery for 2,4,6-TCP in presence of other chlorophenols (e.g. 2,4,5-trichlorophenol and 4-chlorophenol) compared to FFIA alone. (c) 2005 Elsevier B.V. All rights reserved.}},
author = {{Tudorache, Madalina and Emnéus, Jenny}},
issn = {{0376-7388}},
language = {{eng}},
number = {{1-2}},
pages = {{143--149}},
publisher = {{Elsevier}},
series = {{Journal of Membrane Science}},
title = {{Selective immuno-supported liquid membrane (ISLM) extraction, enrichment and analysis of 2,4,6-trichlorophenol}},
url = {{http://dx.doi.org/10.1016/j.memsci.2005.01.045}},
doi = {{10.1016/j.memsci.2005.01.045}},
volume = {{256}},
year = {{2005}},
}