DNA Heats Up: Energetics of Genome Ejection from Phage Revealed by Isothermal Titration Calorimetry.
Jeembaeva, Meerim LU ; Jönsson, Bengt LU ; Castelnovo, Martin and Evilevitch, Alex LU
(2010)
In Journal of Molecular Biology
395(5).
p.1079-1087
- Abstract
- Most bacteriophages are known to inject their double-stranded DNA into bacteria upon receptor binding in an essentially spontaneous way. This downhill thermodynamic process from the intact virion to the empty viral capsid plus released DNA is made possible by the energy stored during active packaging of the genome into the capsid. Only indirect measurements of this energy have been available until now, using either single-molecule or osmotic suppression techniques. In this work, we describe for the first time the use of isothermal titration calorimetry to directly measure the heat released (or, equivalently, the enthalpy) during DNA ejection from phage lambda, triggered in solution by a solubilized receptor. Quantitative analyses of the... (More)
- Most bacteriophages are known to inject their double-stranded DNA into bacteria upon receptor binding in an essentially spontaneous way. This downhill thermodynamic process from the intact virion to the empty viral capsid plus released DNA is made possible by the energy stored during active packaging of the genome into the capsid. Only indirect measurements of this energy have been available until now, using either single-molecule or osmotic suppression techniques. In this work, we describe for the first time the use of isothermal titration calorimetry to directly measure the heat released (or, equivalently, the enthalpy) during DNA ejection from phage lambda, triggered in solution by a solubilized receptor. Quantitative analyses of the results lead to the identification of thermodynamic determinants associated with DNA ejection. The values obtained were found to be consistent with those previously predicted by analytical models and numerical simulations. Moreover, the results confirm the role of DNA hydration in the energetics of genome confinement in viral capsids. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1523830
- author
- Jeembaeva, Meerim
LU
; Jönsson, Bengt
LU
; Castelnovo, Martin
and Evilevitch, Alex
LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Molecular Biology
- volume
- 395
- issue
- 5
- pages
- 1079 - 1087
- publisher
- Elsevier
- external identifiers
-
- wos:000274922400015
- pmid:19969001
- scopus:73649136056
- pmid:19969001
- ISSN
- 1089-8638
- DOI
- 10.1016/j.jmb.2009.11.069
- language
- English
- LU publication?
- yes
- id
- bcc7683d-27a9-44cc-bb09-92f7e2e6f1f9 (old id 1523830)
- date added to LUP
- 2016-04-01 12:52:57
- date last changed
- 2022-03-29 03:52:29
@article{bcc7683d-27a9-44cc-bb09-92f7e2e6f1f9,
abstract = {{Most bacteriophages are known to inject their double-stranded DNA into bacteria upon receptor binding in an essentially spontaneous way. This downhill thermodynamic process from the intact virion to the empty viral capsid plus released DNA is made possible by the energy stored during active packaging of the genome into the capsid. Only indirect measurements of this energy have been available until now, using either single-molecule or osmotic suppression techniques. In this work, we describe for the first time the use of isothermal titration calorimetry to directly measure the heat released (or, equivalently, the enthalpy) during DNA ejection from phage lambda, triggered in solution by a solubilized receptor. Quantitative analyses of the results lead to the identification of thermodynamic determinants associated with DNA ejection. The values obtained were found to be consistent with those previously predicted by analytical models and numerical simulations. Moreover, the results confirm the role of DNA hydration in the energetics of genome confinement in viral capsids.}},
author = {{Jeembaeva, Meerim and Jönsson, Bengt and Castelnovo, Martin and Evilevitch, Alex}},
issn = {{1089-8638}},
language = {{eng}},
number = {{5}},
pages = {{1079--1087}},
publisher = {{Elsevier}},
series = {{Journal of Molecular Biology}},
title = {{DNA Heats Up: Energetics of Genome Ejection from Phage Revealed by Isothermal Titration Calorimetry.}},
url = {{http://dx.doi.org/10.1016/j.jmb.2009.11.069}},
doi = {{10.1016/j.jmb.2009.11.069}},
volume = {{395}},
year = {{2010}},
}