A cultivation technique for E-coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor
de Maré, Lena LU ; Velut, Stéphane LU ; Ledung, E ; Cimander, C ; Norrman, B ; Nordberg Karlsson, Eva LU
; Holst, Olle
LU
and Hagander, Per
LU
(2005)
In Biotechnology Letters
27(14).
p.983-990
- Abstract
- A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O-2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/155069
- author
- de Maré, Lena
LU
; Velut, Stéphane
LU
; Ledung, E
; Cimander, C
; Norrman, B
; Nordberg Karlsson, Eva
LU
; Holst, Olle
LU
and Hagander, Per
LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Letters
- volume
- 27
- issue
- 14
- pages
- 983 - 990
- publisher
- Springer
- external identifiers
-
- pmid:16132841
- wos:000231471000004
- scopus:24044490195
- ISSN
- 1573-6776
- DOI
- 10.1007/s10529-005-7844-6
- language
- English
- LU publication?
- yes
- id
- 670db968-2adb-4dd6-9e8e-e8b0d0685c54 (old id 155069)
- date added to LUP
- 2016-04-01 16:13:38
- date last changed
- 2022-04-15 03:05:11
@article{670db968-2adb-4dd6-9e8e-e8b0d0685c54,
abstract = {{A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O-2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.}},
author = {{de Maré, Lena and Velut, Stéphane and Ledung, E and Cimander, C and Norrman, B and Nordberg Karlsson, Eva and Holst, Olle and Hagander, Per}},
issn = {{1573-6776}},
language = {{eng}},
number = {{14}},
pages = {{983--990}},
publisher = {{Springer}},
series = {{Biotechnology Letters}},
title = {{A cultivation technique for E-coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor}},
url = {{http://dx.doi.org/10.1007/s10529-005-7844-6}},
doi = {{10.1007/s10529-005-7844-6}},
volume = {{27}},
year = {{2005}},
}