Affinity binding of cells to cryogel adsorbents with immobilized specific ligands: effect of ligand coupling and matrix architecture
(2005) In Journal of Molecular Recognition 18(1). p.84-93- Abstract
- The capture of human acute mycloid leukemia KG-1 cells expressing the CD34 surface antigen and the fractionation of human blood lymphocytes were evaluated on polyvinyl alcohol (PVA)-cryogel beads and dimethyl acrylamide (DMAAm) monolithic cryogel with immobilized protein A. The affinity ligand (protein A) was chemically coupled to the reactive PVA-cryogel beads and epoxy-derivatized monolithic cryogels through different immobilization techniques and the binding efficiency of the cell surface receptors specific antibody-labeled cells to the gels/beads was determined. The binding of cells to monolithic cryogel was higher (90-95%) compared with cryogel beads (76%). B-lymphocytes, which bound to the protein Acryogel beads, were separated from... (More)
- The capture of human acute mycloid leukemia KG-1 cells expressing the CD34 surface antigen and the fractionation of human blood lymphocytes were evaluated on polyvinyl alcohol (PVA)-cryogel beads and dimethyl acrylamide (DMAAm) monolithic cryogel with immobilized protein A. The affinity ligand (protein A) was chemically coupled to the reactive PVA-cryogel beads and epoxy-derivatized monolithic cryogels through different immobilization techniques and the binding efficiency of the cell surface receptors specific antibody-labeled cells to the gels/beads was determined. The binding of cells to monolithic cryogel was higher (90-95%) compared with cryogel beads (76%). B-lymphocytes, which bound to the protein Acryogel beads, were separated from T-lymphocytes with yields for the two cell types 74 and 85%, respectively. About 91% of the bound B-cells could be recovered without significantly impairing their viability. Our results show differences in the percentage of cell-binding to the immunosorbents caused by ligand density, flow shear forces and bond strength between the cells and the affinity surface once distinct chemical coupling of protein A, size of beads, sequence of antibody binding to protein A adsorbents, morphology and geometry of surface matrices were compared. Copyright (c) 2004 John Wiley T Sons, Ltd. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/155189
- author
- Kumar, Ashok LU ; Rodriguez-Caballero, A ; Plieva, Fatima LU ; Galaev, Igor LU ; Nandakumar, K S ; Kamihira, M ; Holmdahl, Rikard LU ; Orfao, A and Mattiasson, Bo LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Molecular Recognition
- volume
- 18
- issue
- 1
- pages
- 84 - 93
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000227612900006
- scopus:11244305393
- ISSN
- 1099-1352
- DOI
- 10.1002/jmr.693
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Inflammation Research (013212019), Biotechnology (LTH) (011001037)
- id
- c96c8be8-979c-4685-b0d9-d1da4d39ef97 (old id 155189)
- date added to LUP
- 2016-04-01 11:51:46
- date last changed
- 2023-08-15 13:30:54
@article{c96c8be8-979c-4685-b0d9-d1da4d39ef97,
abstract = {{The capture of human acute mycloid leukemia KG-1 cells expressing the CD34 surface antigen and the fractionation of human blood lymphocytes were evaluated on polyvinyl alcohol (PVA)-cryogel beads and dimethyl acrylamide (DMAAm) monolithic cryogel with immobilized protein A. The affinity ligand (protein A) was chemically coupled to the reactive PVA-cryogel beads and epoxy-derivatized monolithic cryogels through different immobilization techniques and the binding efficiency of the cell surface receptors specific antibody-labeled cells to the gels/beads was determined. The binding of cells to monolithic cryogel was higher (90-95%) compared with cryogel beads (76%). B-lymphocytes, which bound to the protein Acryogel beads, were separated from T-lymphocytes with yields for the two cell types 74 and 85%, respectively. About 91% of the bound B-cells could be recovered without significantly impairing their viability. Our results show differences in the percentage of cell-binding to the immunosorbents caused by ligand density, flow shear forces and bond strength between the cells and the affinity surface once distinct chemical coupling of protein A, size of beads, sequence of antibody binding to protein A adsorbents, morphology and geometry of surface matrices were compared. Copyright (c) 2004 John Wiley T Sons, Ltd.}},
author = {{Kumar, Ashok and Rodriguez-Caballero, A and Plieva, Fatima and Galaev, Igor and Nandakumar, K S and Kamihira, M and Holmdahl, Rikard and Orfao, A and Mattiasson, Bo}},
issn = {{1099-1352}},
language = {{eng}},
number = {{1}},
pages = {{84--93}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Molecular Recognition}},
title = {{Affinity binding of cells to cryogel adsorbents with immobilized specific ligands: effect of ligand coupling and matrix architecture}},
url = {{http://dx.doi.org/10.1002/jmr.693}},
doi = {{10.1002/jmr.693}},
volume = {{18}},
year = {{2005}},
}