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A Dibasic Amino Acid Pair Conserved in the Activation Loop Directs Plasma Membrane Localization and Is Necessary for Activity of Plant Type I/II Phosphatidylinositol Phosphate Kinase

Mikami, Koji ; Saavedra, Laura LU ; Hiwatashi, Yuji ; Uji, Toshiki ; Hasebe, Mitsuyasu and Sommarin, Marianne LU (2010) In Plant Physiology 153(3). p.1004-1015
Abstract
Phosphatidylinositol phosphate kinase (PIPK) is an enzyme involved in the regulation of cellular levels of phosphoinositides involved in various physiological processes, such as cytoskeletal organization, ion channel activation, and vesicle trafficking. In animals, research has focused on the modes of activation and function of PIPKs, providing an understanding of the importance of plasma membrane localization. However, it still remains unclear how this issue is regulated in plant PIPKs. Here, we demonstrate that the carboxyl-terminal catalytic domain, which contains the activation loop, is sufficient for plasma membrane localization of PpPIPK1, a type I/II B PIPK from the moss Physcomitrella patens. The importance of the carboxyl-terminal... (More)
Phosphatidylinositol phosphate kinase (PIPK) is an enzyme involved in the regulation of cellular levels of phosphoinositides involved in various physiological processes, such as cytoskeletal organization, ion channel activation, and vesicle trafficking. In animals, research has focused on the modes of activation and function of PIPKs, providing an understanding of the importance of plasma membrane localization. However, it still remains unclear how this issue is regulated in plant PIPKs. Here, we demonstrate that the carboxyl-terminal catalytic domain, which contains the activation loop, is sufficient for plasma membrane localization of PpPIPK1, a type I/II B PIPK from the moss Physcomitrella patens. The importance of the carboxyl-terminal catalytic domain for plasma membrane localization was confirmed with Arabidopsis (Arabidopsis thaliana) AtPIP5K1. Our findings, in which substitution of a conserved dibasic amino acid pair in the activation loop of PpPIPK1 completely prevented plasma membrane targeting and abolished enzymatic activity, demonstrate its critical role in these processes. Placing our results in the context of studies of eukaryotic PIPKs led us to conclude that the function of the dibasic amino acid pair in the activation loop in type I/II PIPKs is plant specific. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Physiology
volume
153
issue
3
pages
1004 - 1015
publisher
American Society of Plant Biologists
external identifiers
  • wos:000279400200010
  • scopus:77954305924
  • pmid:20427464
ISSN
1532-2548
DOI
10.1104/pp.109.152686
language
English
LU publication?
yes
id
e5b4644f-c1a6-4161-9430-73599912b3b3 (old id 1628709)
date added to LUP
2016-04-01 10:37:48
date last changed
2022-03-27 18:07:16
@article{e5b4644f-c1a6-4161-9430-73599912b3b3,
  abstract     = {{Phosphatidylinositol phosphate kinase (PIPK) is an enzyme involved in the regulation of cellular levels of phosphoinositides involved in various physiological processes, such as cytoskeletal organization, ion channel activation, and vesicle trafficking. In animals, research has focused on the modes of activation and function of PIPKs, providing an understanding of the importance of plasma membrane localization. However, it still remains unclear how this issue is regulated in plant PIPKs. Here, we demonstrate that the carboxyl-terminal catalytic domain, which contains the activation loop, is sufficient for plasma membrane localization of PpPIPK1, a type I/II B PIPK from the moss Physcomitrella patens. The importance of the carboxyl-terminal catalytic domain for plasma membrane localization was confirmed with Arabidopsis (Arabidopsis thaliana) AtPIP5K1. Our findings, in which substitution of a conserved dibasic amino acid pair in the activation loop of PpPIPK1 completely prevented plasma membrane targeting and abolished enzymatic activity, demonstrate its critical role in these processes. Placing our results in the context of studies of eukaryotic PIPKs led us to conclude that the function of the dibasic amino acid pair in the activation loop in type I/II PIPKs is plant specific.}},
  author       = {{Mikami, Koji and Saavedra, Laura and Hiwatashi, Yuji and Uji, Toshiki and Hasebe, Mitsuyasu and Sommarin, Marianne}},
  issn         = {{1532-2548}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{1004--1015}},
  publisher    = {{American Society of Plant Biologists}},
  series       = {{Plant Physiology}},
  title        = {{A Dibasic Amino Acid Pair Conserved in the Activation Loop Directs Plasma Membrane Localization and Is Necessary for Activity of Plant Type I/II Phosphatidylinositol Phosphate Kinase}},
  url          = {{http://dx.doi.org/10.1104/pp.109.152686}},
  doi          = {{10.1104/pp.109.152686}},
  volume       = {{153}},
  year         = {{2010}},
}