Loss- and gain-of-function analyses reveal targets of Pax6 in the developing mouse telencephalon.
(2007) In Molecular and Cellular Neuroscience 34(1). p.99-119- Abstract
- Appropriate neurogenesis and patterning of the forebrain requires the transcription factor Pax6, yet it is largely unknown how Pax6 exerts its effects at the molecular level. To characterize Pax6-mediated regulation of gene expression during murine forebrain neurogenesis, we performed microarray analysis with tissue from the dorsal Pax6-dependent telencephalon and the ventral Pax6-negative telencephalon at the onset of neurogenesis (E12) and at mid-neurogenesis (E15) in wild-type and Pax6-deficient mutant littermates. In the Pax6-deficient cortex the expression levels of various transcription factors involved in neurogenesis (like Satb2, Nfia, AP-2 gamma, NeuroD6, Ngn2, Tbr2, Bhlhb5) and the retinoic acid signalling molecule Rlbp1 were... (More)
- Appropriate neurogenesis and patterning of the forebrain requires the transcription factor Pax6, yet it is largely unknown how Pax6 exerts its effects at the molecular level. To characterize Pax6-mediated regulation of gene expression during murine forebrain neurogenesis, we performed microarray analysis with tissue from the dorsal Pax6-dependent telencephalon and the ventral Pax6-negative telencephalon at the onset of neurogenesis (E12) and at mid-neurogenesis (E15) in wild-type and Pax6-deficient mutant littermates. In the Pax6-deficient cortex the expression levels of various transcription factors involved in neurogenesis (like Satb2, Nfia, AP-2 gamma, NeuroD6, Ngn2, Tbr2, Bhlhb5) and the retinoic acid signalling molecule Rlbp1 were reduced. Regulation by Pax6 could be confirmed upon electroporation of a Pax6- and a dominant-negative Pax6-containing vector into embryonic cortex. Taken together, our data reveal novel insights into the molecular pathways regulated by Pax6 during cortical neurogenesis. Most intriguingly, this analysis revealed time- and region-specific differences in Pax6-mediated transcription, explaining the specific function of Pax6 at early and later stages of neurogenesis. (c) 2006 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/164122
- author
- Holm, Pontus LU ; Mader, Michael T ; Haubst, Nicole ; Wizenmann, Andrea ; Sigvardsson, Mikael LU and Gotz, Magdalena
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- neurogenesis, retinoic acid signalling, electroporation, cortex, Pax6, microarray, telencephalon, ganglionic eminence, small eye
- in
- Molecular and Cellular Neuroscience
- volume
- 34
- issue
- 1
- pages
- 99 - 119
- publisher
- Elsevier
- external identifiers
-
- wos:000243598200011
- scopus:33845915509
- ISSN
- 1044-7431
- DOI
- 10.1016/j.mcn.2006.10.008
- language
- English
- LU publication?
- yes
- id
- 37e92264-ede4-4188-8f16-5d0cb96435b8 (old id 164122)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17158062&dopt=Abstract
- date added to LUP
- 2016-04-01 11:57:30
- date last changed
- 2022-07-08 01:34:29
@article{37e92264-ede4-4188-8f16-5d0cb96435b8,
abstract = {{Appropriate neurogenesis and patterning of the forebrain requires the transcription factor Pax6, yet it is largely unknown how Pax6 exerts its effects at the molecular level. To characterize Pax6-mediated regulation of gene expression during murine forebrain neurogenesis, we performed microarray analysis with tissue from the dorsal Pax6-dependent telencephalon and the ventral Pax6-negative telencephalon at the onset of neurogenesis (E12) and at mid-neurogenesis (E15) in wild-type and Pax6-deficient mutant littermates. In the Pax6-deficient cortex the expression levels of various transcription factors involved in neurogenesis (like Satb2, Nfia, AP-2 gamma, NeuroD6, Ngn2, Tbr2, Bhlhb5) and the retinoic acid signalling molecule Rlbp1 were reduced. Regulation by Pax6 could be confirmed upon electroporation of a Pax6- and a dominant-negative Pax6-containing vector into embryonic cortex. Taken together, our data reveal novel insights into the molecular pathways regulated by Pax6 during cortical neurogenesis. Most intriguingly, this analysis revealed time- and region-specific differences in Pax6-mediated transcription, explaining the specific function of Pax6 at early and later stages of neurogenesis. (c) 2006 Elsevier Inc. All rights reserved.}},
author = {{Holm, Pontus and Mader, Michael T and Haubst, Nicole and Wizenmann, Andrea and Sigvardsson, Mikael and Gotz, Magdalena}},
issn = {{1044-7431}},
keywords = {{neurogenesis; retinoic acid signalling; electroporation; cortex; Pax6; microarray; telencephalon; ganglionic eminence; small eye}},
language = {{eng}},
number = {{1}},
pages = {{99--119}},
publisher = {{Elsevier}},
series = {{Molecular and Cellular Neuroscience}},
title = {{Loss- and gain-of-function analyses reveal targets of Pax6 in the developing mouse telencephalon.}},
url = {{http://dx.doi.org/10.1016/j.mcn.2006.10.008}},
doi = {{10.1016/j.mcn.2006.10.008}},
volume = {{34}},
year = {{2007}},
}