Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Increased cystatin C expression in the pre-eclamptic placenta.

Kristensen, Karl LU ; Larsson, Irene LU and Hansson, Stefan LU orcid (2007) In Molecular Human Reproduction 13. p.189-195
Abstract
Trophoblast invasion is regulated by proteinases and their inhibitors. Cystatin C inhibits cysteine proteinases. The serum concentration of cystatin C is increased in late pregnancy and pre-eclampsia. We aimed to investigate whether the expression of cystatin C is increased in the pre-eclamptic placenta and to investigate the expression pattern of cystatin C mRNA and protein in placental tissue. Tissue samples from the central part of the placenta from 13 normal and 22 pre-eclamptic pregnancies were included. We used real-time polymerase chain reaction (RT-PCR) and in situ hybridization for mRNA expression analysis and immunohistochemistry and Western blotting for protein expression analysis. RT-PCR showed a significantly higher expression... (More)
Trophoblast invasion is regulated by proteinases and their inhibitors. Cystatin C inhibits cysteine proteinases. The serum concentration of cystatin C is increased in late pregnancy and pre-eclampsia. We aimed to investigate whether the expression of cystatin C is increased in the pre-eclamptic placenta and to investigate the expression pattern of cystatin C mRNA and protein in placental tissue. Tissue samples from the central part of the placenta from 13 normal and 22 pre-eclamptic pregnancies were included. We used real-time polymerase chain reaction (RT-PCR) and in situ hybridization for mRNA expression analysis and immunohistochemistry and Western blotting for protein expression analysis. RT-PCR showed a significantly higher expression of cystatin C mRNA in pre-eclampsia than in normal pregnancy, with the highest expression in cases with severe pre-eclampsia. In situ hybridization revealed a distinct pattern of high expression in the extravillous trophoblast cells of the basal plate and low expression in the syncytiotrophoblast covering villi. The cystatin C protein distribution matched the mRNA expression pattern. Western blot analysis revealed an increased protein expression in cases with severe pre-eclampsia and confirmed the presence of cystatin C in amniotic fluid samples. The high expression of cystatin C mRNA in the extravillous trophoblast cells of the basal plate suggests a role for cystatin C in the regulation of proteases in placentation. Placental expression and secretion of cystatin C could contribute to the elevated maternal plasma levels seen in pre-eclampsia. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
placentation, amniotic fluid, proteinase inhibitor, trophoblast
in
Molecular Human Reproduction
volume
13
pages
189 - 195
publisher
Oxford University Press
external identifiers
  • wos:000244662300007
  • scopus:33847678167
ISSN
1460-2407
DOI
10.1093/molehr/gal111
language
English
LU publication?
yes
id
64c9e27a-7b4d-4464-b5a4-41de36c8edd1 (old id 164877)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17227816&dopt=Abstract
date added to LUP
2016-04-01 11:49:50
date last changed
2022-04-13 01:51:52
@article{64c9e27a-7b4d-4464-b5a4-41de36c8edd1,
  abstract     = {{Trophoblast invasion is regulated by proteinases and their inhibitors. Cystatin C inhibits cysteine proteinases. The serum concentration of cystatin C is increased in late pregnancy and pre-eclampsia. We aimed to investigate whether the expression of cystatin C is increased in the pre-eclamptic placenta and to investigate the expression pattern of cystatin C mRNA and protein in placental tissue. Tissue samples from the central part of the placenta from 13 normal and 22 pre-eclamptic pregnancies were included. We used real-time polymerase chain reaction (RT-PCR) and in situ hybridization for mRNA expression analysis and immunohistochemistry and Western blotting for protein expression analysis. RT-PCR showed a significantly higher expression of cystatin C mRNA in pre-eclampsia than in normal pregnancy, with the highest expression in cases with severe pre-eclampsia. In situ hybridization revealed a distinct pattern of high expression in the extravillous trophoblast cells of the basal plate and low expression in the syncytiotrophoblast covering villi. The cystatin C protein distribution matched the mRNA expression pattern. Western blot analysis revealed an increased protein expression in cases with severe pre-eclampsia and confirmed the presence of cystatin C in amniotic fluid samples. The high expression of cystatin C mRNA in the extravillous trophoblast cells of the basal plate suggests a role for cystatin C in the regulation of proteases in placentation. Placental expression and secretion of cystatin C could contribute to the elevated maternal plasma levels seen in pre-eclampsia.}},
  author       = {{Kristensen, Karl and Larsson, Irene and Hansson, Stefan}},
  issn         = {{1460-2407}},
  keywords     = {{placentation; amniotic fluid; proteinase inhibitor; trophoblast}},
  language     = {{eng}},
  pages        = {{189--195}},
  publisher    = {{Oxford University Press}},
  series       = {{Molecular Human Reproduction}},
  title        = {{Increased cystatin C expression in the pre-eclamptic placenta.}},
  url          = {{http://dx.doi.org/10.1093/molehr/gal111}},
  doi          = {{10.1093/molehr/gal111}},
  volume       = {{13}},
  year         = {{2007}},
}