Adenoviral vectors for transient gene expression in human primitive hematopoietic cells: Applications and prospects.
Järås, Marcus LU ; Brun, Ann LU ; Karlsson, Stefan LU
and Fan, Xiaolong
LU
(2007)
In Experimental Hematology
35(3).
p.343-349
- Abstract
- The proliferation and differentiation of primitive hematopoietic cells is tightly controlled by a number of signaling pathways. Transient blockage or enhancement of these signaling pathways may provide a new approach to manipulate the proliferation and differentiation of primitive hematopoietic cells. Adenoviral vectors have in recent years emerged as powerful tools for transient gene expression in human primitive hematopoietic cells. Important advantageous properties of adenoviral vectors include: feasible production of high-titer vector preparations, high efficiency in transducing both quiescent and actively dividing cells, high levels of transient gene expression, and a lack of mutagenic properties associated with integrating vectors.... (More)
- The proliferation and differentiation of primitive hematopoietic cells is tightly controlled by a number of signaling pathways. Transient blockage or enhancement of these signaling pathways may provide a new approach to manipulate the proliferation and differentiation of primitive hematopoietic cells. Adenoviral vectors have in recent years emerged as powerful tools for transient gene expression in human primitive hematopoietic cells. Important advantageous properties of adenoviral vectors include: feasible production of high-titer vector preparations, high efficiency in transducing both quiescent and actively dividing cells, high levels of transient gene expression, and a lack of mutagenic properties associated with integrating vectors. Progress in adenoviral fiber retargeting was recently demonstrated to enable high gene transfer efficiency into nondividing human CD34(+) cells and nonobese diabetic/severe combined immunodeficient mouse bone marrow repopulating cells (SRCs), via the ubiquitously expressed CD46 as a cellular receptor. Importantly, fiber-retargeted adenoviral vectors can be engineered to report gene expression in single living CD34(+) cells, thereby facilitating the isolation and characterization of SRCs and its downstream progenitors based on intrinsic signaling pathways. This review focuses on the current progress and the potential future applications of adenoviral gene transfer into human primitive hematopoietic cells and leukemic cells. (c) 2007 International Society for Experimental Hematology. Published by Elsevier Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/165575
- author
- Järås, Marcus
LU
; Brun, Ann
LU
; Karlsson, Stefan
LU
and Fan, Xiaolong
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Experimental Hematology
- volume
- 35
- issue
- 3
- pages
- 343 - 349
- publisher
- Elsevier
- external identifiers
-
- wos:000244605200001
- scopus:33847037957
- ISSN
- 1873-2399
- DOI
- 10.1016/j.exphem.2006.11.004
- language
- English
- LU publication?
- yes
- id
- f87ea47c-2ee6-4878-a6ed-18b274eb1a83 (old id 165575)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17309814&dopt=Abstract
- date added to LUP
- 2016-04-01 11:57:42
- date last changed
- 2022-01-26 20:49:26
@article{f87ea47c-2ee6-4878-a6ed-18b274eb1a83,
abstract = {{The proliferation and differentiation of primitive hematopoietic cells is tightly controlled by a number of signaling pathways. Transient blockage or enhancement of these signaling pathways may provide a new approach to manipulate the proliferation and differentiation of primitive hematopoietic cells. Adenoviral vectors have in recent years emerged as powerful tools for transient gene expression in human primitive hematopoietic cells. Important advantageous properties of adenoviral vectors include: feasible production of high-titer vector preparations, high efficiency in transducing both quiescent and actively dividing cells, high levels of transient gene expression, and a lack of mutagenic properties associated with integrating vectors. Progress in adenoviral fiber retargeting was recently demonstrated to enable high gene transfer efficiency into nondividing human CD34(+) cells and nonobese diabetic/severe combined immunodeficient mouse bone marrow repopulating cells (SRCs), via the ubiquitously expressed CD46 as a cellular receptor. Importantly, fiber-retargeted adenoviral vectors can be engineered to report gene expression in single living CD34(+) cells, thereby facilitating the isolation and characterization of SRCs and its downstream progenitors based on intrinsic signaling pathways. This review focuses on the current progress and the potential future applications of adenoviral gene transfer into human primitive hematopoietic cells and leukemic cells. (c) 2007 International Society for Experimental Hematology. Published by Elsevier Inc.}},
author = {{Järås, Marcus and Brun, Ann and Karlsson, Stefan and Fan, Xiaolong}},
issn = {{1873-2399}},
language = {{eng}},
number = {{3}},
pages = {{343--349}},
publisher = {{Elsevier}},
series = {{Experimental Hematology}},
title = {{Adenoviral vectors for transient gene expression in human primitive hematopoietic cells: Applications and prospects.}},
url = {{http://dx.doi.org/10.1016/j.exphem.2006.11.004}},
doi = {{10.1016/j.exphem.2006.11.004}},
volume = {{35}},
year = {{2007}},
}