Effects of l-arginine on cerebral blood flow, microvascular permeability, number of perfused capillaries, and brain water content in the traumatized mouse brain.
(2007) In Microvascular Research 74. p.1-8- Abstract
- It is has been suggested that decreased production of the vasodilatory and anti-aggregative substance NO (nitric oxide) may result in lower cerebral blood flow (CBF) in injured areas of the traumatized brain. The NO-precursor L-arginine has been shown to counteract CBF decreases early after trauma, but microcirculatory and more long-term effects on CBF of L-arginine have not been investigated. In an attempt to analyze effects Of L-arginine on the microcirculation in the traumatized brain, the present study was designed to evaluate the effects Of L-arginine compared to vehicle (0.9% saline) following a standardized controlled cortical-impact brain trauma in mice. Cerebral blood flow (autoradiography [C-14]-iodoantipyrine), number of... (More)
- It is has been suggested that decreased production of the vasodilatory and anti-aggregative substance NO (nitric oxide) may result in lower cerebral blood flow (CBF) in injured areas of the traumatized brain. The NO-precursor L-arginine has been shown to counteract CBF decreases early after trauma, but microcirculatory and more long-term effects on CBF of L-arginine have not been investigated. In an attempt to analyze effects Of L-arginine on the microcirculation in the traumatized brain, the present study was designed to evaluate the effects Of L-arginine compared to vehicle (0.9% saline) following a standardized controlled cortical-impact brain trauma in mice. Cerebral blood flow (autoradiography [C-14]-iodoantipyrine), number of perfused capillaries (FITC-dextran fluorescence technique), brain water content (wet vs. dry weight) and the blood to brain transfer constant K-i for [Cr-51]-EDTA were analyzed in the injured and the contralateral cortex. Cortical blood flow in the injured cortex was 0.43 +/- 0.3 mL/g/min and 0.8 +/- 0.3 mL/g/min 3 h after trauma in the vehicle and L-arginine groups, respectively (p < 0.05), and no treatment effect was seen 24 h after trauma. The number of perfused capillaries decreased following trauma and was unaffected by L-arginine. Ki increased following trauma and was unaffected by L-arginine. Brain water content was lower in the L-arginine group than in the vehicle group 3 h after trauma and there was no difference between the groups 24 h after trauma. We conclude that L-arginine reduces brain edema formation and improves cortical blood flow in the early phase after a brain trauma, whereas no circulatory effects can be seen after prolonged treatment. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/167365
- author
- Lundblad, Cornelia LU and Bentzer, Peter LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Microvascular Research
- volume
- 74
- pages
- 1 - 8
- publisher
- Academic Press
- external identifiers
-
- wos:000247715000001
- scopus:34249813108
- ISSN
- 1095-9319
- DOI
- 10.1016/j.mvr.2007.03.001
- language
- English
- LU publication?
- yes
- id
- 0c7a68be-d3d8-445e-bd3d-1a04a268dc7a (old id 167365)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17459424&dopt=Abstract
- date added to LUP
- 2016-04-01 12:08:43
- date last changed
- 2022-03-28 20:56:31
@article{0c7a68be-d3d8-445e-bd3d-1a04a268dc7a,
abstract = {{It is has been suggested that decreased production of the vasodilatory and anti-aggregative substance NO (nitric oxide) may result in lower cerebral blood flow (CBF) in injured areas of the traumatized brain. The NO-precursor L-arginine has been shown to counteract CBF decreases early after trauma, but microcirculatory and more long-term effects on CBF of L-arginine have not been investigated. In an attempt to analyze effects Of L-arginine on the microcirculation in the traumatized brain, the present study was designed to evaluate the effects Of L-arginine compared to vehicle (0.9% saline) following a standardized controlled cortical-impact brain trauma in mice. Cerebral blood flow (autoradiography [C-14]-iodoantipyrine), number of perfused capillaries (FITC-dextran fluorescence technique), brain water content (wet vs. dry weight) and the blood to brain transfer constant K-i for [Cr-51]-EDTA were analyzed in the injured and the contralateral cortex. Cortical blood flow in the injured cortex was 0.43 +/- 0.3 mL/g/min and 0.8 +/- 0.3 mL/g/min 3 h after trauma in the vehicle and L-arginine groups, respectively (p < 0.05), and no treatment effect was seen 24 h after trauma. The number of perfused capillaries decreased following trauma and was unaffected by L-arginine. Ki increased following trauma and was unaffected by L-arginine. Brain water content was lower in the L-arginine group than in the vehicle group 3 h after trauma and there was no difference between the groups 24 h after trauma. We conclude that L-arginine reduces brain edema formation and improves cortical blood flow in the early phase after a brain trauma, whereas no circulatory effects can be seen after prolonged treatment.}},
author = {{Lundblad, Cornelia and Bentzer, Peter}},
issn = {{1095-9319}},
language = {{eng}},
pages = {{1--8}},
publisher = {{Academic Press}},
series = {{Microvascular Research}},
title = {{Effects of l-arginine on cerebral blood flow, microvascular permeability, number of perfused capillaries, and brain water content in the traumatized mouse brain.}},
url = {{http://dx.doi.org/10.1016/j.mvr.2007.03.001}},
doi = {{10.1016/j.mvr.2007.03.001}},
volume = {{74}},
year = {{2007}},
}