Transient and sustained increases in glomerular permeability following ANP infusion in rats.
(2011) In American Journal of Physiology-Renal Physiology 300. p.24-30- Abstract
- The present study was performed to investigate the effects of systemic atrial natriuretic peptide (ANP) infusion on the glomerular permeability to macromolecules in rats. In anaesthetized Wistar rats (250-280g) the left urether was cannulated for urine collection while simultaneously blood access was achieved. Rats were continuously infused i.v. with ANP, 30 ng/min/kg (Lo-ANP; n=8) or 800 ng/min/kg (Hi-ANP; n=10) or 0.9% NaCl (SHAM; n=16), respectively, and with polydisperse fluorescein isothiocyanate (FITC)-Ficoll-70/400 (mol.radius 13-90Å) and (51)Cr-EDTA for 2 h. Plasma and urine samples were taken at 5, 15, 30, 60 and 120 min of ANP infusion, and analyzed by high performance size exclusion chromatography (HPLC) for determination of... (More)
- The present study was performed to investigate the effects of systemic atrial natriuretic peptide (ANP) infusion on the glomerular permeability to macromolecules in rats. In anaesthetized Wistar rats (250-280g) the left urether was cannulated for urine collection while simultaneously blood access was achieved. Rats were continuously infused i.v. with ANP, 30 ng/min/kg (Lo-ANP; n=8) or 800 ng/min/kg (Hi-ANP; n=10) or 0.9% NaCl (SHAM; n=16), respectively, and with polydisperse fluorescein isothiocyanate (FITC)-Ficoll-70/400 (mol.radius 13-90Å) and (51)Cr-EDTA for 2 h. Plasma and urine samples were taken at 5, 15, 30, 60 and 120 min of ANP infusion, and analyzed by high performance size exclusion chromatography (HPLC) for determination of glomerular sieving coefficients () for Ficoll. GFR was also assessed ((51)Cr-EDTA). In Hi-ANP there was a rapid (within 5 min), but bimodal, increase in glomerular permeability. to high MW Ficoll thus reached a maximum at 15 min, after which returned to near control at 30 min, to again increase moderately at 60 and 120 min. In Lo-ANP there was also a rapid, reversible increase in glomerular , returning to near control at 30 min, followed by just a tendency of a sustained increase in permeability, but with a significant increase in "large pore" radius. In conclusion, in Hi-ANP there was a rapid increase in glomerular permeability, with an early, partly reversible permeability peak, followed by a (moderate) sustained increase in permeability. In Lo-ANP animals, only the initial permeability peak was evident. In both Lo-ANP and Hi-ANP the glomerular sieving pattern observed was found to mainly reflect an increase in the number and radius of "large pores" in the glomerular filter. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1711205
- author
- Axelsson, Josefin LU ; Rippe, Anna LU and Rippe, Bengt LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- American Journal of Physiology-Renal Physiology
- volume
- 300
- pages
- 24 - 30
- publisher
- American Physiological Society
- external identifiers
-
- wos:000285964000003
- pmid:20943768
- scopus:78651300320
- ISSN
- 1522-1466
- DOI
- 10.1152/ajprenal.00347.2010
- language
- English
- LU publication?
- yes
- id
- e9b65ba2-3cb4-4c1c-937d-caf608f78e5f (old id 1711205)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20943768?dopt=Abstract
- date added to LUP
- 2016-04-04 08:12:45
- date last changed
- 2022-02-20 21:23:51
@article{e9b65ba2-3cb4-4c1c-937d-caf608f78e5f,
abstract = {{The present study was performed to investigate the effects of systemic atrial natriuretic peptide (ANP) infusion on the glomerular permeability to macromolecules in rats. In anaesthetized Wistar rats (250-280g) the left urether was cannulated for urine collection while simultaneously blood access was achieved. Rats were continuously infused i.v. with ANP, 30 ng/min/kg (Lo-ANP; n=8) or 800 ng/min/kg (Hi-ANP; n=10) or 0.9% NaCl (SHAM; n=16), respectively, and with polydisperse fluorescein isothiocyanate (FITC)-Ficoll-70/400 (mol.radius 13-90Å) and (51)Cr-EDTA for 2 h. Plasma and urine samples were taken at 5, 15, 30, 60 and 120 min of ANP infusion, and analyzed by high performance size exclusion chromatography (HPLC) for determination of glomerular sieving coefficients () for Ficoll. GFR was also assessed ((51)Cr-EDTA). In Hi-ANP there was a rapid (within 5 min), but bimodal, increase in glomerular permeability. to high MW Ficoll thus reached a maximum at 15 min, after which returned to near control at 30 min, to again increase moderately at 60 and 120 min. In Lo-ANP there was also a rapid, reversible increase in glomerular , returning to near control at 30 min, followed by just a tendency of a sustained increase in permeability, but with a significant increase in "large pore" radius. In conclusion, in Hi-ANP there was a rapid increase in glomerular permeability, with an early, partly reversible permeability peak, followed by a (moderate) sustained increase in permeability. In Lo-ANP animals, only the initial permeability peak was evident. In both Lo-ANP and Hi-ANP the glomerular sieving pattern observed was found to mainly reflect an increase in the number and radius of "large pores" in the glomerular filter.}},
author = {{Axelsson, Josefin and Rippe, Anna and Rippe, Bengt}},
issn = {{1522-1466}},
language = {{eng}},
pages = {{24--30}},
publisher = {{American Physiological Society}},
series = {{American Journal of Physiology-Renal Physiology}},
title = {{Transient and sustained increases in glomerular permeability following ANP infusion in rats.}},
url = {{http://dx.doi.org/10.1152/ajprenal.00347.2010}},
doi = {{10.1152/ajprenal.00347.2010}},
volume = {{300}},
year = {{2011}},
}