Transscleral visible/near-infrared spectroscopy for quantitative assessment of haemoglobin in experimental choroidal tumours.
(2012) In Acta Ophthalmologica 90. p.350-356- Abstract
- Purpose: To study the feasibility of using transscleral visible/near-infrared spectroscopy (Vis/NIRS) to estimate the content of haemoglobin in choroidal tumour phantoms of ex vivo porcine eyes. Methods: Thirty enucleated porcine eyes were prepared with a tumour phantom made by injecting a suspension of gelatine, titanium dioxide and human blood into the suprachoroidal space. The blood concentrations used were 2.5%, 25% and 50%, with 10 eyes in each group. Alternating Vis/NIRS measurements were taken over the phantom inclusion and on the opposite (normal) side of each eye. For statistical analysis, a genetic algorithm was utilized to suppress insignificant wavelengths in the spectra. The processed spectra were then used to build a... (More)
- Purpose: To study the feasibility of using transscleral visible/near-infrared spectroscopy (Vis/NIRS) to estimate the content of haemoglobin in choroidal tumour phantoms of ex vivo porcine eyes. Methods: Thirty enucleated porcine eyes were prepared with a tumour phantom made by injecting a suspension of gelatine, titanium dioxide and human blood into the suprachoroidal space. The blood concentrations used were 2.5%, 25% and 50%, with 10 eyes in each group. Alternating Vis/NIRS measurements were taken over the phantom inclusion and on the opposite (normal) side of each eye. For statistical analysis, a genetic algorithm was utilized to suppress insignificant wavelengths in the spectra. The processed spectra were then used to build a regression model based on partial least squares regression and evaluated by twofold cross-validation. Results: Ultrasonography revealed that all phantoms were localized within the suprachoroidal space with no penetration through the retina. The largest mean diameters of the phantoms with 2.5%, 25% and 50% blood were 15.5, 15.2 and 15.7 mm, respectively (p > 0.05). The largest mean thicknesses were 4.5, 4.5 and 4.8 mm, respectively (p > 0.05). Statistical analysis of the spectral data showed that it was possible to correctly discriminate between the normal side and the tumour phantom side of the eyes in 99.88% of cases. The phantoms could be correctly classified according to their blood concentrations in 99.42% of cases. Conclusions: This study demonstrates that transscleral Vis/NIRS is a feasible and accurate method for the detection of choroidal tumours and to assess the haemoglobin content in such lesions. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1756439
- author
- Xu, Can LU ; Svenmarker, Pontus LU ; Andersson-Engels, Stefan LU and Krohn, Jørgen
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Ophthalmologica
- volume
- 90
- pages
- 350 - 356
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000306903100030
- pmid:21155981
- scopus:84861592690
- pmid:21155981
- ISSN
- 1755-3768
- DOI
- 10.1111/j.1755-3768.2010.02037.x
- language
- English
- LU publication?
- yes
- id
- d610459b-f0e7-4b09-a79b-be5191130a4f (old id 1756439)
- date added to LUP
- 2016-04-01 10:47:11
- date last changed
- 2022-01-26 02:26:57
@article{d610459b-f0e7-4b09-a79b-be5191130a4f,
abstract = {{Purpose: To study the feasibility of using transscleral visible/near-infrared spectroscopy (Vis/NIRS) to estimate the content of haemoglobin in choroidal tumour phantoms of ex vivo porcine eyes. Methods: Thirty enucleated porcine eyes were prepared with a tumour phantom made by injecting a suspension of gelatine, titanium dioxide and human blood into the suprachoroidal space. The blood concentrations used were 2.5%, 25% and 50%, with 10 eyes in each group. Alternating Vis/NIRS measurements were taken over the phantom inclusion and on the opposite (normal) side of each eye. For statistical analysis, a genetic algorithm was utilized to suppress insignificant wavelengths in the spectra. The processed spectra were then used to build a regression model based on partial least squares regression and evaluated by twofold cross-validation. Results: Ultrasonography revealed that all phantoms were localized within the suprachoroidal space with no penetration through the retina. The largest mean diameters of the phantoms with 2.5%, 25% and 50% blood were 15.5, 15.2 and 15.7 mm, respectively (p > 0.05). The largest mean thicknesses were 4.5, 4.5 and 4.8 mm, respectively (p > 0.05). Statistical analysis of the spectral data showed that it was possible to correctly discriminate between the normal side and the tumour phantom side of the eyes in 99.88% of cases. The phantoms could be correctly classified according to their blood concentrations in 99.42% of cases. Conclusions: This study demonstrates that transscleral Vis/NIRS is a feasible and accurate method for the detection of choroidal tumours and to assess the haemoglobin content in such lesions.}},
author = {{Xu, Can and Svenmarker, Pontus and Andersson-Engels, Stefan and Krohn, Jørgen}},
issn = {{1755-3768}},
language = {{eng}},
pages = {{350--356}},
publisher = {{Wiley-Blackwell}},
series = {{Acta Ophthalmologica}},
title = {{Transscleral visible/near-infrared spectroscopy for quantitative assessment of haemoglobin in experimental choroidal tumours.}},
url = {{https://lup.lub.lu.se/search/files/2136383/2370942.pdf}},
doi = {{10.1111/j.1755-3768.2010.02037.x}},
volume = {{90}},
year = {{2012}},
}