The miR-17-92 MicroRNA Cluster Regulates Multiple Components of the TGF-beta Pathway in Neuroblastoma
(2010) In Molecular Cell 40(5). p.762-773- Abstract
- The miR-17-92 microRNA cluster is often activated in cancer cells, but the identity of its targets remains elusive. Using SILAC and quantitative mass spectrometry, we examined the effects of activation of the miR-17-92 cluster on global protein expression in neuroblastoma (NB) cells. Our results reveal cooperation between individual miR-17-92 miRNAs and implicate miR-17-92 in multiple hallmarks of cancer, including proliferation and cell adhesion. Most importantly, we show that miR-17-92 is a potent inhibitor of TGF-beta signaling. By functioning both upstream and downstream of pSMAD2, miR-17-92 activation triggers downregulation of multiple key effectors along the TGF-beta signaling cascade as well as direct inhibition of... (More)
- The miR-17-92 microRNA cluster is often activated in cancer cells, but the identity of its targets remains elusive. Using SILAC and quantitative mass spectrometry, we examined the effects of activation of the miR-17-92 cluster on global protein expression in neuroblastoma (NB) cells. Our results reveal cooperation between individual miR-17-92 miRNAs and implicate miR-17-92 in multiple hallmarks of cancer, including proliferation and cell adhesion. Most importantly, we show that miR-17-92 is a potent inhibitor of TGF-beta signaling. By functioning both upstream and downstream of pSMAD2, miR-17-92 activation triggers downregulation of multiple key effectors along the TGF-beta signaling cascade as well as direct inhibition of TGF-beta-responsive genes. (Less)
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https://lup.lub.lu.se/record/1771167
- author
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular Cell
- volume
- 40
- issue
- 5
- pages
- 762 - 773
- publisher
- Cell Press
- external identifiers
-
- wos:000285405800010
- scopus:78649941638
- pmid:21145484
- ISSN
- 1097-4164
- DOI
- 10.1016/j.molcel.2010.11.038
- language
- English
- LU publication?
- yes
- additional info
- Department affilation moved from v1000583 (Molecular Tumour Biology) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:41:47.
- id
- 9393d28d-67f9-4b43-8bfb-e84e229fbf83 (old id 1771167)
- date added to LUP
- 2016-04-01 10:20:36
- date last changed
- 2022-04-27 21:12:50
@article{9393d28d-67f9-4b43-8bfb-e84e229fbf83, abstract = {{The miR-17-92 microRNA cluster is often activated in cancer cells, but the identity of its targets remains elusive. Using SILAC and quantitative mass spectrometry, we examined the effects of activation of the miR-17-92 cluster on global protein expression in neuroblastoma (NB) cells. Our results reveal cooperation between individual miR-17-92 miRNAs and implicate miR-17-92 in multiple hallmarks of cancer, including proliferation and cell adhesion. Most importantly, we show that miR-17-92 is a potent inhibitor of TGF-beta signaling. By functioning both upstream and downstream of pSMAD2, miR-17-92 activation triggers downregulation of multiple key effectors along the TGF-beta signaling cascade as well as direct inhibition of TGF-beta-responsive genes.}}, author = {{Mestdagh, Pieter and Boström, Anna-Karin and Impens, Francis and Fredlund, Erik and Van Peer, Gert and De Antonellis, Pasqualino and von Stedingk, Kristoffer and Ghesquiere, Bart and Schulte, Stefanie and Dews, Michael and Thomas-Tikhonenko, Andrei and Schulte, Johannes H. and Zollo, Massimo and Schramm, Alexander and Gevaert, Kris and Axelson, Håkan and Speleman, Frank and Vandesompele, Jo}}, issn = {{1097-4164}}, language = {{eng}}, number = {{5}}, pages = {{762--773}}, publisher = {{Cell Press}}, series = {{Molecular Cell}}, title = {{The miR-17-92 MicroRNA Cluster Regulates Multiple Components of the TGF-beta Pathway in Neuroblastoma}}, url = {{http://dx.doi.org/10.1016/j.molcel.2010.11.038}}, doi = {{10.1016/j.molcel.2010.11.038}}, volume = {{40}}, year = {{2010}}, }