Minimal residual disease assessment in childhood acute lymphoblastic leukaemia: a Swedish multi-centre study comparing real-time polymerase chain reaction and multicolour flow cytometry.
(2011) In British Journal of Haematology 152(6). p.743-753- Abstract
- Minimal residual disease (MRD) assessment is a powerful prognostic factor for determining the risk of relapse in childhood acute lymphoblastic leukaemia (ALL). In this Swedish multi-centre study of childhood ALL diagnosed between 2002 and 2006, the MRD levels were analysed in 726 follow-up samples in 228 children using real-time quantitative polymerase chain reaction (RQ-PCR) of rearranged immunoglobulin/T-cell receptor genes and multicolour flow cytometry (FCM). Using an MRD threshold of 0·1%, which was the sensitivity level reached in all analyses, the concordance between RQ-PCR and FCM MRD values at day 29 was 84%. In B-cell precursor ALL, an MRD level of ≥0·1% at day 29 predicted a higher risk of bone marrow relapse (BMR) with both... (More)
- Minimal residual disease (MRD) assessment is a powerful prognostic factor for determining the risk of relapse in childhood acute lymphoblastic leukaemia (ALL). In this Swedish multi-centre study of childhood ALL diagnosed between 2002 and 2006, the MRD levels were analysed in 726 follow-up samples in 228 children using real-time quantitative polymerase chain reaction (RQ-PCR) of rearranged immunoglobulin/T-cell receptor genes and multicolour flow cytometry (FCM). Using an MRD threshold of 0·1%, which was the sensitivity level reached in all analyses, the concordance between RQ-PCR and FCM MRD values at day 29 was 84%. In B-cell precursor ALL, an MRD level of ≥0·1% at day 29 predicted a higher risk of bone marrow relapse (BMR) with both methods, although FCM was a better discriminator. However, considering the higher median MRD values achieved with RQ-PCR, a higher MRD cut-off (≥0·2%) improved the predictive capacity of RQ-PCR. In T-ALL, RQ-PCR was notably superior to FCM in predicting risk of BMR. That notwithstanding, MRD levels of ≥0·1%, detected by either method at day 29, could not predict isolated extramedullary relapse. In conclusion, the concordance between RQ-PCR and FCM was high and hence both methods are valuable clinical tools for identifying childhood ALL cases with increased risk of BMR. (Less)
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https://lup.lub.lu.se/record/1777237
- author
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- childhood acute lymphoblastic leukaemia, disease, minimal residual, TCR genes, rearranged IG, flow cytometry, RQ-PCR
- in
- British Journal of Haematology
- volume
- 152
- issue
- 6
- pages
- 743 - 753
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000287739500008
- pmid:21250970
- scopus:79952008832
- pmid:21250970
- ISSN
- 0007-1048
- DOI
- 10.1111/j.1365-2141.2010.08456.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Family Medicine (013241010), Division of Hematology and Transfusion Medicine (013041100), Paediatrics (Lund) (013002000), Psychiatry/Primary Care/Public Health (013240500)
- id
- 4ace96e1-6de7-45b8-87b3-1cbc2caf7293 (old id 1777237)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21250970?dopt=Abstract
- date added to LUP
- 2016-04-01 11:15:01
- date last changed
- 2022-08-20 18:23:54
@article{4ace96e1-6de7-45b8-87b3-1cbc2caf7293,
abstract = {{Minimal residual disease (MRD) assessment is a powerful prognostic factor for determining the risk of relapse in childhood acute lymphoblastic leukaemia (ALL). In this Swedish multi-centre study of childhood ALL diagnosed between 2002 and 2006, the MRD levels were analysed in 726 follow-up samples in 228 children using real-time quantitative polymerase chain reaction (RQ-PCR) of rearranged immunoglobulin/T-cell receptor genes and multicolour flow cytometry (FCM). Using an MRD threshold of 0·1%, which was the sensitivity level reached in all analyses, the concordance between RQ-PCR and FCM MRD values at day 29 was 84%. In B-cell precursor ALL, an MRD level of ≥0·1% at day 29 predicted a higher risk of bone marrow relapse (BMR) with both methods, although FCM was a better discriminator. However, considering the higher median MRD values achieved with RQ-PCR, a higher MRD cut-off (≥0·2%) improved the predictive capacity of RQ-PCR. In T-ALL, RQ-PCR was notably superior to FCM in predicting risk of BMR. That notwithstanding, MRD levels of ≥0·1%, detected by either method at day 29, could not predict isolated extramedullary relapse. In conclusion, the concordance between RQ-PCR and FCM was high and hence both methods are valuable clinical tools for identifying childhood ALL cases with increased risk of BMR.}},
author = {{Thörnerup, Ingrid and Forestier, Erik and Botling, Johan and Thuresson, Britt and Wasslavik, Carina and Björklund, Elisabet and Li, Aihong and Lindström-Eriksson, Eleonor and Malec, Maria and Grönlund, Elisabeth and Torikka, Kerstin and Heldrup, Jesper and Abrahamsson, Jonas and Behrendtz, Mikael and Söderhäll, Stefan and Jacobsson, Stefan and Olofsson, Tor and Porwit, Anna and Lönnerholm, Gudmar and Rosenquist, Richard and Sundström, Christer}},
issn = {{0007-1048}},
keywords = {{childhood acute lymphoblastic leukaemia; disease; minimal residual; TCR genes; rearranged IG; flow cytometry; RQ-PCR}},
language = {{eng}},
number = {{6}},
pages = {{743--753}},
publisher = {{Wiley-Blackwell}},
series = {{British Journal of Haematology}},
title = {{Minimal residual disease assessment in childhood acute lymphoblastic leukaemia: a Swedish multi-centre study comparing real-time polymerase chain reaction and multicolour flow cytometry.}},
url = {{http://dx.doi.org/10.1111/j.1365-2141.2010.08456.x}},
doi = {{10.1111/j.1365-2141.2010.08456.x}},
volume = {{152}},
year = {{2011}},
}