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Comparison of (GTG)(5)-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates

Ryberg, Anna ; Olsson, Crister LU ; Ahrné, Siv LU and Monstein, Hans-Juerg (2011) In Journal of Microbiological Methods 84(2). p.183-188
Abstract
Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis... (More)
Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis revealed that K pneumoniae and K oxytoca isolates. reference and type strains formed distinct cluster groups, and tentative subclusters could be established. We conclude that (GTG)(5) and ITS-PCR analysis combined with automated capillary electrophoresis provides promising tools for molecular typing of Klebsiella isolates. (C) 2010 Elsevier B.V. All rights reserved. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Klebsiella spp., Molecular typing, (GTG)(5)-PCR, Ribosomal intergenic, transcribed spacer (ITS)-PCR, Fingerprint analysis, Multiple, displacement amplification
in
Journal of Microbiological Methods
volume
84
issue
2
pages
183 - 188
publisher
Elsevier
external identifiers
  • wos:000287951200005
  • scopus:79251613122
  • pmid:21129413
ISSN
1872-8359
DOI
10.1016/j.mimet.2010.11.019
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Applied Nutrition and Food Chemistry (011001300), Surgery Research Unit (013242220)
id
c0191bc8-d2cf-4ff2-ac07-5915eeb8d0f4 (old id 1869174)
date added to LUP
2016-04-01 11:04:21
date last changed
2023-09-14 18:47:23
@article{c0191bc8-d2cf-4ff2-ac07-5915eeb8d0f4,
  abstract     = {{Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis revealed that K pneumoniae and K oxytoca isolates. reference and type strains formed distinct cluster groups, and tentative subclusters could be established. We conclude that (GTG)(5) and ITS-PCR analysis combined with automated capillary electrophoresis provides promising tools for molecular typing of Klebsiella isolates. (C) 2010 Elsevier B.V. All rights reserved.}},
  author       = {{Ryberg, Anna and Olsson, Crister and Ahrné, Siv and Monstein, Hans-Juerg}},
  issn         = {{1872-8359}},
  keywords     = {{Klebsiella spp.; Molecular typing; (GTG)(5)-PCR; Ribosomal intergenic; transcribed spacer (ITS)-PCR; Fingerprint analysis; Multiple; displacement amplification}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{183--188}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Microbiological Methods}},
  title        = {{Comparison of (GTG)(5)-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates}},
  url          = {{http://dx.doi.org/10.1016/j.mimet.2010.11.019}},
  doi          = {{10.1016/j.mimet.2010.11.019}},
  volume       = {{84}},
  year         = {{2011}},
}