Antiproliferative Activity of Melanoidins Isolated from Heated Potato Fiber (Potex) in Glioma Cell Culture Model
(2011) In Journal of Agricultural and Food Chemistry 59(6). p.2708-2716- Abstract
- Potex constitutes a potato fiber preparation widely used as an ingredient to meat and bakery products which thermal treatment results in creation of new compounds. Melanoidins are high molecular weight brown end products of Maillard reaction, and few data presenting tumor cell growth inhibiting activity of melanoidins have been reported. Thus, in present study we utilized water extract of Potex roasted (180 degrees C for 2 h), whose chemical characterization revealed the presence of melanoidin complexes. Heated Potex extract inhibited C6 glioma cell proliferation in a dose-dependent manner measured by MTT method. High molecular weight components present in initial extract were responsible for stronger antiproliferative effect compared with... (More)
- Potex constitutes a potato fiber preparation widely used as an ingredient to meat and bakery products which thermal treatment results in creation of new compounds. Melanoidins are high molecular weight brown end products of Maillard reaction, and few data presenting tumor cell growth inhibiting activity of melanoidins have been reported. Thus, in present study we utilized water extract of Potex roasted (180 degrees C for 2 h), whose chemical characterization revealed the presence of melanoidin complexes. Heated Potex extract inhibited C6 glioma cell proliferation in a dose-dependent manner measured by MTT method. High molecular weight components present in initial extract were responsible for stronger antiproliferative effect compared with low molecular weight fraction. Impaired MAPK (mitogen-activated protein kinase) and Akt signaling was found in cells treated with the extract. Moreover, flow cytometry analyses revealed the extract to induce G(1)/S arrest in glioma cells. Simultaneously, Western blot analysis showed elevated levels of p21 protein with concomitant decrease of cyclin D1. In conclusion, observed antiproliferative activity of melanoidins present in heated Potex was linked to disregulated MAPK and Akt signaling pathways, as well as to cell cycle cessation. These results suggest potential application of Potex preparation as a functional food ingredient and chemopreventive agent. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1936192
- author
- Langner, Ewa ; Nunes, Fernando M. ; Pozarowski, Piotr ; Kandefer-Szerszen, Martyna ; Pierzynowski, Stefan LU and Rzeski, Wojciech
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- antiproliferative effect, cell cycle, melanoidins, potato fiber, protein kinase signaling
- in
- Journal of Agricultural and Food Chemistry
- volume
- 59
- issue
- 6
- pages
- 2708 - 2716
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000288401800072
- scopus:79952763816
- pmid:21341669
- ISSN
- 0021-8561
- DOI
- 10.1021/jf1047223
- language
- English
- LU publication?
- yes
- id
- 67aafd3c-8763-4487-a4e9-3f4ae962ee5a (old id 1936192)
- date added to LUP
- 2016-04-01 11:07:53
- date last changed
- 2022-01-26 05:38:59
@article{67aafd3c-8763-4487-a4e9-3f4ae962ee5a,
abstract = {{Potex constitutes a potato fiber preparation widely used as an ingredient to meat and bakery products which thermal treatment results in creation of new compounds. Melanoidins are high molecular weight brown end products of Maillard reaction, and few data presenting tumor cell growth inhibiting activity of melanoidins have been reported. Thus, in present study we utilized water extract of Potex roasted (180 degrees C for 2 h), whose chemical characterization revealed the presence of melanoidin complexes. Heated Potex extract inhibited C6 glioma cell proliferation in a dose-dependent manner measured by MTT method. High molecular weight components present in initial extract were responsible for stronger antiproliferative effect compared with low molecular weight fraction. Impaired MAPK (mitogen-activated protein kinase) and Akt signaling was found in cells treated with the extract. Moreover, flow cytometry analyses revealed the extract to induce G(1)/S arrest in glioma cells. Simultaneously, Western blot analysis showed elevated levels of p21 protein with concomitant decrease of cyclin D1. In conclusion, observed antiproliferative activity of melanoidins present in heated Potex was linked to disregulated MAPK and Akt signaling pathways, as well as to cell cycle cessation. These results suggest potential application of Potex preparation as a functional food ingredient and chemopreventive agent.}},
author = {{Langner, Ewa and Nunes, Fernando M. and Pozarowski, Piotr and Kandefer-Szerszen, Martyna and Pierzynowski, Stefan and Rzeski, Wojciech}},
issn = {{0021-8561}},
keywords = {{antiproliferative effect; cell cycle; melanoidins; potato fiber; protein kinase signaling}},
language = {{eng}},
number = {{6}},
pages = {{2708--2716}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Journal of Agricultural and Food Chemistry}},
title = {{Antiproliferative Activity of Melanoidins Isolated from Heated Potato Fiber (Potex) in Glioma Cell Culture Model}},
url = {{http://dx.doi.org/10.1021/jf1047223}},
doi = {{10.1021/jf1047223}},
volume = {{59}},
year = {{2011}},
}