Hsa-miR-34b is a plasma-stable microRNA that is elevated in pre-manifest Huntington's disease
Gaughwin, Philip LU ; Ciesla, Maciej ; Lahiri, Nayana ; Tabrizi, Sarah J. ; Brundin, Patrik LU and Björkqvist, Maria LU
(2011)
In Human Molecular Genetics
20(11).
p.2225-2237
- Abstract
- Huntington's disease (HD) is a devastating, neurodegenerative condition, which lacks effective treatment. Normal Huntingtin (HTT) and mutant Huntingtin (mHTT) are expressed in multiple tissues and can alter transcription of microRNAs (miRs). Importantly, miRs are present in a bio-stable form in human peripheral blood plasma and have recently been shown to be useful biomarkers in other diseases. We therefore sought to identify potential miR biomarkers of HD that are present in, and have functional consequences for, neuronal and non-neuronal tissues. In a cell line over-expressing mHTT-Exon-1, miR microarray analysis was used to identify candidate miRs. We then examined their presence and bio-stability in control and HD plasma. We found that... (More)
- Huntington's disease (HD) is a devastating, neurodegenerative condition, which lacks effective treatment. Normal Huntingtin (HTT) and mutant Huntingtin (mHTT) are expressed in multiple tissues and can alter transcription of microRNAs (miRs). Importantly, miRs are present in a bio-stable form in human peripheral blood plasma and have recently been shown to be useful biomarkers in other diseases. We therefore sought to identify potential miR biomarkers of HD that are present in, and have functional consequences for, neuronal and non-neuronal tissues. In a cell line over-expressing mHTT-Exon-1, miR microarray analysis was used to identify candidate miRs. We then examined their presence and bio-stability in control and HD plasma. We found that miR-34b is significantly elevated in response to mHTT-Exon-1, and its blockade alters the toxicity of mHTT-Exon-1 in vitro. We also show that miR-34b is detectable in plasma from small input volumes and is insensitive to freeze-thaw-induced RNA degradation. Interestingly, miR-34b is significantly elevated in plasma from HD gene carriers prior to symptom onset. This is the first study suggesting that plasma miRs might be used as biomarkers for HD. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1986863
- author
- Gaughwin, Philip
LU
; Ciesla, Maciej
; Lahiri, Nayana
; Tabrizi, Sarah J.
; Brundin, Patrik
LU
and Björkqvist, Maria
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Human Molecular Genetics
- volume
- 20
- issue
- 11
- pages
- 2225 - 2237
- publisher
- Oxford University Press
- external identifiers
-
- wos:000290589800013
- scopus:79956032718
- pmid:21421997
- pmid:21421997
- ISSN
- 0964-6906
- DOI
- 10.1093/hmg/ddr111
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuronal Survival (013212041)
- id
- 7f013389-4bd0-4b11-be91-59d9bfee343d (old id 1986863)
- date added to LUP
- 2016-04-01 10:41:57
- date last changed
- 2023-08-31 09:05:55
@article{7f013389-4bd0-4b11-be91-59d9bfee343d,
abstract = {{Huntington's disease (HD) is a devastating, neurodegenerative condition, which lacks effective treatment. Normal Huntingtin (HTT) and mutant Huntingtin (mHTT) are expressed in multiple tissues and can alter transcription of microRNAs (miRs). Importantly, miRs are present in a bio-stable form in human peripheral blood plasma and have recently been shown to be useful biomarkers in other diseases. We therefore sought to identify potential miR biomarkers of HD that are present in, and have functional consequences for, neuronal and non-neuronal tissues. In a cell line over-expressing mHTT-Exon-1, miR microarray analysis was used to identify candidate miRs. We then examined their presence and bio-stability in control and HD plasma. We found that miR-34b is significantly elevated in response to mHTT-Exon-1, and its blockade alters the toxicity of mHTT-Exon-1 in vitro. We also show that miR-34b is detectable in plasma from small input volumes and is insensitive to freeze-thaw-induced RNA degradation. Interestingly, miR-34b is significantly elevated in plasma from HD gene carriers prior to symptom onset. This is the first study suggesting that plasma miRs might be used as biomarkers for HD.}},
author = {{Gaughwin, Philip and Ciesla, Maciej and Lahiri, Nayana and Tabrizi, Sarah J. and Brundin, Patrik and Björkqvist, Maria}},
issn = {{0964-6906}},
language = {{eng}},
number = {{11}},
pages = {{2225--2237}},
publisher = {{Oxford University Press}},
series = {{Human Molecular Genetics}},
title = {{Hsa-miR-34b is a plasma-stable microRNA that is elevated in pre-manifest Huntington's disease}},
url = {{http://dx.doi.org/10.1093/hmg/ddr111}},
doi = {{10.1093/hmg/ddr111}},
volume = {{20}},
year = {{2011}},
}