The GTPase domain of gamma-tubulin is required for normal mitochondrial function and spatial organization
Lindström, Lisa LU ; Li, Tongbin ; Malycheva, Darina LU ; Kancharla, Arun ; Nilsson, Helén LU ; Vishnu, Neelanjan LU ; Mulder, Hindrik LU
; Johansson, Martin
LU
; Rosselló, Catalina Ana
and Alvarado-Kristensson, Maria
LU
(2018)
In Communications Biology
1(https://doi.org/10.1038/s42003-018-0037-3).
- Abstract
- In the cell, γ-tubulin establishes a cellular network of threads named the γ-string meshwork. However, the functions of this meshwork remain to be determined. We investigated the traits of the meshwork and show that γ-strings have the ability to connect the cytoplasm and the mitochondrial DNA together. We also show that γ-tubulin has a role in the maintenance of the mitochondrial network and functions as reduced levels of γ-tubulin or impairment of its GTPase domain disrupts the mitochondrial network and alters both their respiratory capacity and the expression of mitochondrial-related genes. By contrast, reduced mitochondrial number or increased protein levels of γ-tubulin DNA-binding domain enhanced the association of γ-tubulin with... (More)
- In the cell, γ-tubulin establishes a cellular network of threads named the γ-string meshwork. However, the functions of this meshwork remain to be determined. We investigated the traits of the meshwork and show that γ-strings have the ability to connect the cytoplasm and the mitochondrial DNA together. We also show that γ-tubulin has a role in the maintenance of the mitochondrial network and functions as reduced levels of γ-tubulin or impairment of its GTPase domain disrupts the mitochondrial network and alters both their respiratory capacity and the expression of mitochondrial-related genes. By contrast, reduced mitochondrial number or increased protein levels of γ-tubulin DNA-binding domain enhanced the association of γ-tubulin with mitochondria. Our results demonstrate that γ-tubulin is an important mitochondrial structural component that maintains the mitochondrial network, providing mitochondria with a cellular infrastructure. We propose that γ-tubulin provides a cytoskeletal element that gives form to the mitochondrial network. (Less)
- Abstract (Swedish)
- In the cell, γ-tubulin establishes a cellular network of threads named the γ-string meshwork. However, the functions of this meshwork remain to be determined. We investigated the traits of the meshwork and show that γ-strings have the ability to connect the cytoplasm and the mitochondrial DNA together. We also show that γ-tubulin has a role in the maintenance of the mitochondrial network and functions as reduced levels of γ-tubulin or impairment of its GTPase domain disrupts the mitochondrial network and alters both their respiratory capacity and the expression of mitochondrial-related genes. By contrast, reduced mitochondrial number or increased protein levels of γ-tubulin DNA-binding domain enhanced the association of γ-tubulin with... (More)
- In the cell, γ-tubulin establishes a cellular network of threads named the γ-string meshwork. However, the functions of this meshwork remain to be determined. We investigated the traits of the meshwork and show that γ-strings have the ability to connect the cytoplasm and the mitochondrial DNA together. We also show that γ-tubulin has a role in the maintenance of the mitochondrial network and functions as reduced levels of γ-tubulin or impairment of its GTPase domain disrupts the mitochondrial network and alters both their respiratory capacity and the expression of mitochondrial-related genes. By contrast, reduced mitochondrial number or increased protein levels of γ-tubulin DNA-binding domain enhanced the association of γ-tubulin with mitochondria. Our results demonstrate that γ-tubulin is an important mitochondrial structural component that maintains the mitochondrial network, providing mitochondria with a cellular infrastructure. We propose that γ-tubulin provides a cytoskeletal element that gives form to the mitochondrial network. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1a191f20-68d3-4f6c-b672-08f529a97c71
- author
- Lindström, Lisa
LU
; Li, Tongbin
; Malycheva, Darina
LU
; Kancharla, Arun
; Nilsson, Helén
LU
; Vishnu, Neelanjan
LU
; Mulder, Hindrik
LU
; Johansson, Martin
LU
; Rosselló, Catalina Ana
and Alvarado-Kristensson, Maria
LU
- organization
- publishing date
- 2018-05-03
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Communications Biology
- volume
- 1
- issue
- https://doi.org/10.1038/s42003-018-0037-3
- article number
- 37
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:30271923
- scopus:85055429802
- ISSN
- 2399-3642
- DOI
- 10.1038/s42003-018-0037-3
- language
- English
- LU publication?
- yes
- id
- 1a191f20-68d3-4f6c-b672-08f529a97c71
- date added to LUP
- 2018-08-27 13:37:47
- date last changed
- 2022-05-03 05:29:07
@article{1a191f20-68d3-4f6c-b672-08f529a97c71,
abstract = {{In the cell, γ-tubulin establishes a cellular network of threads named the γ-string meshwork. However, the functions of this meshwork remain to be determined. We investigated the traits of the meshwork and show that γ-strings have the ability to connect the cytoplasm and the mitochondrial DNA together. We also show that γ-tubulin has a role in the maintenance of the mitochondrial network and functions as reduced levels of γ-tubulin or impairment of its GTPase domain disrupts the mitochondrial network and alters both their respiratory capacity and the expression of mitochondrial-related genes. By contrast, reduced mitochondrial number or increased protein levels of γ-tubulin DNA-binding domain enhanced the association of γ-tubulin with mitochondria. Our results demonstrate that γ-tubulin is an important mitochondrial structural component that maintains the mitochondrial network, providing mitochondria with a cellular infrastructure. We propose that γ-tubulin provides a cytoskeletal element that gives form to the mitochondrial network.}},
author = {{Lindström, Lisa and Li, Tongbin and Malycheva, Darina and Kancharla, Arun and Nilsson, Helén and Vishnu, Neelanjan and Mulder, Hindrik and Johansson, Martin and Rosselló, Catalina Ana and Alvarado-Kristensson, Maria}},
issn = {{2399-3642}},
language = {{eng}},
month = {{05}},
number = {{https://doi.org/10.1038/s42003-018-0037-3}},
publisher = {{Nature Publishing Group}},
series = {{Communications Biology}},
title = {{The GTPase domain of gamma-tubulin is required for normal mitochondrial function and spatial organization}},
url = {{http://dx.doi.org/10.1038/s42003-018-0037-3}},
doi = {{10.1038/s42003-018-0037-3}},
volume = {{1}},
year = {{2018}},
}