Coupled diffusion and adsorption effects for multiple proteins in agarose gel
(2004) In AIChE Journal 50(12). p.3006-3018- Abstract
- A mathematical model for the hindered diffusion and competitive adsorption of two proteins in an agarose gel has been developed. In a simulation program the model has been used to study the competing and displacement effects on a single bead for lysozyme and bovine serum albumin (BSA) bound to the ligand Cibacron Blue in agarose gel. The model takes into account hindered diffusion described by the Renkin model, competitive Langmuir adsorption kinetics, pore size distribution of the gel, and a shrinking effective pore radius attributed to molecule-to-ligand binding. The simulation model can easily explain displacement of BSA or lysozyme dependent on the binding capacity, kinetics, and diffusion. The influence of a bimodal pore size... (More)
- A mathematical model for the hindered diffusion and competitive adsorption of two proteins in an agarose gel has been developed. In a simulation program the model has been used to study the competing and displacement effects on a single bead for lysozyme and bovine serum albumin (BSA) bound to the ligand Cibacron Blue in agarose gel. The model takes into account hindered diffusion described by the Renkin model, competitive Langmuir adsorption kinetics, pore size distribution of the gel, and a shrinking effective pore radius attributed to molecule-to-ligand binding. The simulation model can easily explain displacement of BSA or lysozyme dependent on the binding capacity, kinetics, and diffusion. The influence of a bimodal pore size distribution is demonstrated. It also provides insight into the phenomenon of static vs. dynamic binding capacity observed in experimental determinations of the isotherm. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/141700
- author
- Gutenwik, Jan LU ; Nilsson, Bernt LU and Axelsson, Anders LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- AIChE Journal
- volume
- 50
- issue
- 12
- pages
- 3006 - 3018
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000225445000003
- scopus:10844275580
- ISSN
- 1547-5905
- DOI
- 10.1002/aic.10231
- language
- English
- LU publication?
- yes
- id
- 1abb3af6-fabd-4472-b062-c512f6fd5db7 (old id 141700)
- date added to LUP
- 2016-04-01 11:50:40
- date last changed
- 2023-10-13 12:17:42
@article{1abb3af6-fabd-4472-b062-c512f6fd5db7,
abstract = {{A mathematical model for the hindered diffusion and competitive adsorption of two proteins in an agarose gel has been developed. In a simulation program the model has been used to study the competing and displacement effects on a single bead for lysozyme and bovine serum albumin (BSA) bound to the ligand Cibacron Blue in agarose gel. The model takes into account hindered diffusion described by the Renkin model, competitive Langmuir adsorption kinetics, pore size distribution of the gel, and a shrinking effective pore radius attributed to molecule-to-ligand binding. The simulation model can easily explain displacement of BSA or lysozyme dependent on the binding capacity, kinetics, and diffusion. The influence of a bimodal pore size distribution is demonstrated. It also provides insight into the phenomenon of static vs. dynamic binding capacity observed in experimental determinations of the isotherm.}},
author = {{Gutenwik, Jan and Nilsson, Bernt and Axelsson, Anders}},
issn = {{1547-5905}},
language = {{eng}},
number = {{12}},
pages = {{3006--3018}},
publisher = {{John Wiley & Sons Inc.}},
series = {{AIChE Journal}},
title = {{Coupled diffusion and adsorption effects for multiple proteins in agarose gel}},
url = {{http://dx.doi.org/10.1002/aic.10231}},
doi = {{10.1002/aic.10231}},
volume = {{50}},
year = {{2004}},
}