Virion glycosylation governs integrity and infectivity of infectious pancreatic necrosis virus.
(2011) In Journal of Fish Diseases 34(9). p.663-675- Abstract
- The possible importance of the O-linked glycosylation in virion stability and infectivity of infectious pancreatic necrosis virus (IPNV) was analysed. Enzymatic treatment with O-glycosidase of radiolabelled virions under different ionic conditions, to allow for possible alternative exposure of glycosidic enzyme cleavage sites, did not alter the specific infectivity of virions re-isolated after rate-zonal centrifugation in glycerol gradients. As an alternative method to assess the significance of carbohydrates in IPNV integrity, periodate oxidation in the presence of an aldehyde quencher was chosen. Following re-isolation of viruses, a 3-5 (10) log-unit reduction in specific infectivity was revealed and, at higher concentrations, a total... (More)
- The possible importance of the O-linked glycosylation in virion stability and infectivity of infectious pancreatic necrosis virus (IPNV) was analysed. Enzymatic treatment with O-glycosidase of radiolabelled virions under different ionic conditions, to allow for possible alternative exposure of glycosidic enzyme cleavage sites, did not alter the specific infectivity of virions re-isolated after rate-zonal centrifugation in glycerol gradients. As an alternative method to assess the significance of carbohydrates in IPNV integrity, periodate oxidation in the presence of an aldehyde quencher was chosen. Following re-isolation of viruses, a 3-5 (10) log-unit reduction in specific infectivity was revealed and, at higher concentrations, a total disruption or virion aggregation was observed. The loss of infectivity of intact virions was not because of a lack of attachment to cells. Additionally, re-evaluation of reading values from UV-spectra of purified IPNV yielded a specific infectivity of 3 10(11) TCID(50) -units mg(-1) of protein and a ratio of 40 virions per TCID(50) -unit in the CHSE-214 cell system. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2151109
- author
- Fridholm, Helena LU and Everitt, Einar LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Fish Diseases
- volume
- 34
- issue
- 9
- pages
- 663 - 675
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000293913000003
- pmid:21838710
- scopus:80051656765
- pmid:21838710
- ISSN
- 0140-7775
- DOI
- 10.1111/j.1365-2761.2011.01280.x
- language
- English
- LU publication?
- yes
- id
- 6c998578-612d-408a-8be9-c68b1db93e2d (old id 2151109)
- date added to LUP
- 2016-04-01 11:02:46
- date last changed
- 2022-01-26 04:54:02
@article{6c998578-612d-408a-8be9-c68b1db93e2d,
abstract = {{The possible importance of the O-linked glycosylation in virion stability and infectivity of infectious pancreatic necrosis virus (IPNV) was analysed. Enzymatic treatment with O-glycosidase of radiolabelled virions under different ionic conditions, to allow for possible alternative exposure of glycosidic enzyme cleavage sites, did not alter the specific infectivity of virions re-isolated after rate-zonal centrifugation in glycerol gradients. As an alternative method to assess the significance of carbohydrates in IPNV integrity, periodate oxidation in the presence of an aldehyde quencher was chosen. Following re-isolation of viruses, a 3-5 (10) log-unit reduction in specific infectivity was revealed and, at higher concentrations, a total disruption or virion aggregation was observed. The loss of infectivity of intact virions was not because of a lack of attachment to cells. Additionally, re-evaluation of reading values from UV-spectra of purified IPNV yielded a specific infectivity of 3 10(11) TCID(50) -units mg(-1) of protein and a ratio of 40 virions per TCID(50) -unit in the CHSE-214 cell system.}},
author = {{Fridholm, Helena and Everitt, Einar}},
issn = {{0140-7775}},
language = {{eng}},
number = {{9}},
pages = {{663--675}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Fish Diseases}},
title = {{Virion glycosylation governs integrity and infectivity of infectious pancreatic necrosis virus.}},
url = {{http://dx.doi.org/10.1111/j.1365-2761.2011.01280.x}},
doi = {{10.1111/j.1365-2761.2011.01280.x}},
volume = {{34}},
year = {{2011}},
}