Immunophenotypical characterization of human neutrophil differentiation
(2011) In Journal of Leukocyte Biology 90(3). p.629-634- Abstract
- The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid... (More)
- The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs. J. Leukoc. Biol. 90: 629-634; 2011. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2159335
- author
- Mora-Jensen, Helena ; Jendholm, Johan ; Fossum, Anna ; Porse, Bo ; Borregaad, Niels and Theilgaard-Moench, Kim LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- granulocytes, myeloid differentiation, differentiation pathway, flow, cytometry, human bone marrow
- in
- Journal of Leukocyte Biology
- volume
- 90
- issue
- 3
- pages
- 629 - 634
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000294441700023
- scopus:80052364253
- pmid:21653237
- ISSN
- 1938-3673
- DOI
- 10.1189/jlb.0311123
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematology/Transplantation (013022014), Stem Cell Center (013022011)
- id
- 4fa517d2-e12d-43b0-9f57-a4a0b05ab6a6 (old id 2159335)
- date added to LUP
- 2016-04-01 10:55:58
- date last changed
- 2022-08-05 19:11:21
@article{4fa517d2-e12d-43b0-9f57-a4a0b05ab6a6,
abstract = {{The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs. J. Leukoc. Biol. 90: 629-634; 2011.}},
author = {{Mora-Jensen, Helena and Jendholm, Johan and Fossum, Anna and Porse, Bo and Borregaad, Niels and Theilgaard-Moench, Kim}},
issn = {{1938-3673}},
keywords = {{granulocytes; myeloid differentiation; differentiation pathway; flow; cytometry; human bone marrow}},
language = {{eng}},
number = {{3}},
pages = {{629--634}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Leukocyte Biology}},
title = {{Immunophenotypical characterization of human neutrophil differentiation}},
url = {{http://dx.doi.org/10.1189/jlb.0311123}},
doi = {{10.1189/jlb.0311123}},
volume = {{90}},
year = {{2011}},
}