Design and development of electrochemical sensors with application in bioanalysis
(2011)- Abstract
- There is a continuous search for reliable, robust, easy to use, portable and cost effective analytical tools with applications in food industry, biomedicine, environmental monitoring and homeland security.
The main focus of this thesis is the development of electrochemical (bio)sensors and bioanalysis systems (to be applied in two major fields of interest: food & beverage industry and biomedical research) for monitoring key analytes in complex matrices, such as: meat samples, fermentation broths, and cell culture media.
The proposed monitoring methods for calpastatin (a meat tenderness biomarker), successfully applied to meat samples, were based on (i) label free capacitive immunosensors and (ii)amperometric... (More) - There is a continuous search for reliable, robust, easy to use, portable and cost effective analytical tools with applications in food industry, biomedicine, environmental monitoring and homeland security.
The main focus of this thesis is the development of electrochemical (bio)sensors and bioanalysis systems (to be applied in two major fields of interest: food & beverage industry and biomedical research) for monitoring key analytes in complex matrices, such as: meat samples, fermentation broths, and cell culture media.
The proposed monitoring methods for calpastatin (a meat tenderness biomarker), successfully applied to meat samples, were based on (i) label free capacitive immunosensors and (ii)amperometric immunoassays. The amperometric immunoassay approach was considered to be used when an electrochemical detection system has been designed, fabricated and applied
for calpastatin quantification from bovine skeletal muscle. The obtained values were correlated with Warner Bratzler Shear Force data, a routinely used method for meat tenderness determination. Glucose and ethanol were simultaneously monitored during a fermentation process with miniaturized enzyme based amperometric biosensors connected to a microdialysis probe.
Besides food analysis applications, the amperometric immunoassay for calpastatin and additionally for μ-calpain detection is very important in biomedicine, both proteins having an important role in the pathology of several diseases. Electrochemical cellular dynamics monitoring has been achieved in real time by using a microfluidic cell culture system, where
the electrodes were integrated at the bottom of the cell culture chambers. (Less) - Abstract (Swedish)
- Popular Abstract in English
There is a continuous search for reliable, robust easy to use, portable and cost effective
analytical tools with applications in food industry, biomedicine, environmental
monitoring and homeland security.
The main focus of the thesis was on the development of electrochemical (bio)sensors
designed to detect analytes of key importance from biological matrixes (e.g.,
fermentation broth, meat samples and cell culture media) applied in two major fields of
interest: food & beverage industry and biomedical research.
Calpastatin, a meat tenderness biomarker, was successfully detected, from meat samples,
with the developed label... (More) - Popular Abstract in English
There is a continuous search for reliable, robust easy to use, portable and cost effective
analytical tools with applications in food industry, biomedicine, environmental
monitoring and homeland security.
The main focus of the thesis was on the development of electrochemical (bio)sensors
designed to detect analytes of key importance from biological matrixes (e.g.,
fermentation broth, meat samples and cell culture media) applied in two major fields of
interest: food & beverage industry and biomedical research.
Calpastatin, a meat tenderness biomarker, was successfully detected, from meat samples,
with the developed label free capacitive immunosensors and amperometric
immunoassays. Based on the amperometric immunoassay an electrochemical detection
system has been designed, fabricated and applied for calpastatin quantification and the
obtained values were correlated with Warner Bratzler Shear Force data, a routinely used
method for meat tenderness determination. Glucose and ethanol were simultaneously
monitored during a fermentation process with miniaturized enzyme based amperometric
biosensors connected to a microdialysis probe.
Beside the application in food analysis, it was shown the possibility of application of
amperometric immunoassay for calpastatin and μ-calpain in biomedicine, both proteins
having an important role in the pathology of several diseases. Electrochemical cellular
dynamics monitoring has been achieved in real time by using a microfluidic cell culture
system where the electrodes were integrated at the bottom of the cell culture chambers. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2168113
- author
- Zor, Kinga LU
- supervisor
-
- Lo Gorton LU
- opponent
-
- Prof. Domínguez, Elena, Department of Analytical Chemistry, University of Alcala, Madrid, Spain
- organization
- publishing date
- 2011
- type
- Thesis
- publication status
- published
- subject
- keywords
- bioanalysis, electrochemistry, sensor, biosensor, miniaturization, real sample, immunoassay, μTAS
- defense location
- Lecture hall B, Centre of Chemistry and Chemical Engineering, Sölvegatan 3, Lund University Faculty of Engineering
- defense date
- 2011-10-29 10:15:00
- language
- English
- LU publication?
- yes
- id
- b6d2b879-0d6d-4b66-90f2-6bdd9f83a6de (old id 2168113)
- date added to LUP
- 2016-04-04 14:26:07
- date last changed
- 2018-11-21 21:20:18
@phdthesis{b6d2b879-0d6d-4b66-90f2-6bdd9f83a6de,
abstract = {{There is a continuous search for reliable, robust, easy to use, portable and cost effective analytical tools with applications in food industry, biomedicine, environmental monitoring and homeland security.<br/><br>
The main focus of this thesis is the development of electrochemical (bio)sensors and bioanalysis systems (to be applied in two major fields of interest: food & beverage industry and biomedical research) for monitoring key analytes in complex matrices, such as: meat samples, fermentation broths, and cell culture media.<br/><br>
The proposed monitoring methods for calpastatin (a meat tenderness biomarker), successfully applied to meat samples, were based on (i) label free capacitive immunosensors and (ii)amperometric immunoassays. The amperometric immunoassay approach was considered to be used when an electrochemical detection system has been designed, fabricated and applied<br/><br>
for calpastatin quantification from bovine skeletal muscle. The obtained values were correlated with Warner Bratzler Shear Force data, a routinely used method for meat tenderness determination. Glucose and ethanol were simultaneously monitored during a fermentation process with miniaturized enzyme based amperometric biosensors connected to a microdialysis probe.<br/><br>
Besides food analysis applications, the amperometric immunoassay for calpastatin and additionally for μ-calpain detection is very important in biomedicine, both proteins having an important role in the pathology of several diseases. Electrochemical cellular dynamics monitoring has been achieved in real time by using a microfluidic cell culture system, where<br/><br>
the electrodes were integrated at the bottom of the cell culture chambers.}},
author = {{Zor, Kinga}},
keywords = {{bioanalysis; electrochemistry; sensor; biosensor; miniaturization; real sample; immunoassay; μTAS}},
language = {{eng}},
school = {{Lund University}},
title = {{Design and development of electrochemical sensors with application in bioanalysis}},
year = {{2011}},
}