Inefficient splicing of segment 7 and 8 mRNAs is an inherent property of influenza virus A/Brevig Mission/1918/1 (H1N1) that causes elevated expression of NS1 protein.
(2012) In Virology 422. p.46-58- Abstract
- Influenza A virus encodes two segments (7 and 8) that produce mRNAs that can be spliced. We have investigated if naturally occurring sequence polymorphisms in the influenza A virus family affects splicing of these viral mRNAs, as that could potentially alter the NS1/NS2- and/or M1/M2-protein ratios. We compared splicing efficiency of segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) and A/Netherlands/178/95 (H3N2), as well as various H5N1 avian strains. Results revealed that both segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) were inefficiently spliced compared to other influenza virus segment 7 and 8 mRNAs. This resulted in production of higher levels of functional NS1 protein, which could potentially contribute to the... (More)
- Influenza A virus encodes two segments (7 and 8) that produce mRNAs that can be spliced. We have investigated if naturally occurring sequence polymorphisms in the influenza A virus family affects splicing of these viral mRNAs, as that could potentially alter the NS1/NS2- and/or M1/M2-protein ratios. We compared splicing efficiency of segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) and A/Netherlands/178/95 (H3N2), as well as various H5N1 avian strains. Results revealed that both segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) were inefficiently spliced compared to other influenza virus segment 7 and 8 mRNAs. This resulted in production of higher levels of functional NS1 protein, which could potentially contribute to the pathogenic properties of the A/Brevig Mission/1918/1 (H1N1). We also show that A/Brevig Mission/1918/1 (H1N1) segment 8 mRNAs responded differently to overexpression of SR proteins than A/Netherlands/178/95 (H3N2). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2221413
- author
- Winquist, Ellenor LU ; Abdurahman, Samir ; Tranell, Anna ; Lindström, Sofia ; Tingsborg, Susanne and Schwartz, Stefan LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Virology
- volume
- 422
- pages
- 46 - 58
- publisher
- Elsevier
- external identifiers
-
- wos:000297902300006
- pmid:22036312
- scopus:82355190150
- pmid:22036312
- ISSN
- 1096-0341
- DOI
- 10.1016/j.virol.2011.10.004
- language
- English
- LU publication?
- yes
- id
- d65fbd37-d0d3-4789-8095-ceea2f13c4db (old id 2221413)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22036312?dopt=Abstract
- date added to LUP
- 2016-04-04 09:19:44
- date last changed
- 2022-04-23 20:02:30
@article{d65fbd37-d0d3-4789-8095-ceea2f13c4db,
abstract = {{Influenza A virus encodes two segments (7 and 8) that produce mRNAs that can be spliced. We have investigated if naturally occurring sequence polymorphisms in the influenza A virus family affects splicing of these viral mRNAs, as that could potentially alter the NS1/NS2- and/or M1/M2-protein ratios. We compared splicing efficiency of segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) and A/Netherlands/178/95 (H3N2), as well as various H5N1 avian strains. Results revealed that both segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) were inefficiently spliced compared to other influenza virus segment 7 and 8 mRNAs. This resulted in production of higher levels of functional NS1 protein, which could potentially contribute to the pathogenic properties of the A/Brevig Mission/1918/1 (H1N1). We also show that A/Brevig Mission/1918/1 (H1N1) segment 8 mRNAs responded differently to overexpression of SR proteins than A/Netherlands/178/95 (H3N2).}},
author = {{Winquist, Ellenor and Abdurahman, Samir and Tranell, Anna and Lindström, Sofia and Tingsborg, Susanne and Schwartz, Stefan}},
issn = {{1096-0341}},
language = {{eng}},
pages = {{46--58}},
publisher = {{Elsevier}},
series = {{Virology}},
title = {{Inefficient splicing of segment 7 and 8 mRNAs is an inherent property of influenza virus A/Brevig Mission/1918/1 (H1N1) that causes elevated expression of NS1 protein.}},
url = {{http://dx.doi.org/10.1016/j.virol.2011.10.004}},
doi = {{10.1016/j.virol.2011.10.004}},
volume = {{422}},
year = {{2012}},
}