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Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus

Chalot, Michel ; Brun, Annick ; Finlay, Roger D. and Söderström, Bengt LU (1994) In FEMS Microbiology Letters 121(1). p.87-91
Abstract
The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amino acid respiration, Aminooxyacetate, [14C]alanine, Ectomycorrhizal fungus, Paxillus involutus, TCA cycle
in
FEMS Microbiology Letters
volume
121
issue
1
pages
87 - 91
publisher
Oxford University Press
external identifiers
  • scopus:0028176327
ISSN
1574-6968
DOI
10.1111/j.1574-6968.1994.tb07080.x
language
English
LU publication?
yes
id
413b9026-d67e-4c8a-9cc8-ed72ef940e5b (old id 2226321)
date added to LUP
2016-04-01 11:40:18
date last changed
2021-01-03 04:40:19
@article{413b9026-d67e-4c8a-9cc8-ed72ef940e5b,
  abstract     = {{The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.}},
  author       = {{Chalot, Michel and Brun, Annick and Finlay, Roger D. and Söderström, Bengt}},
  issn         = {{1574-6968}},
  keywords     = {{Amino acid respiration; Aminooxyacetate; [14C]alanine; Ectomycorrhizal fungus; Paxillus involutus; TCA cycle}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{87--91}},
  publisher    = {{Oxford University Press}},
  series       = {{FEMS Microbiology Letters}},
  title        = {{Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus}},
  url          = {{http://dx.doi.org/10.1111/j.1574-6968.1994.tb07080.x}},
  doi          = {{10.1111/j.1574-6968.1994.tb07080.x}},
  volume       = {{121}},
  year         = {{1994}},
}