An oat branmeal influences blood insulin levels and related gene sets in peripheral blood mononuclear cells of healthy subjects
Ulmius, Matilda LU ; Johansson Persson, Anna LU ; Krogh, Morten LU ; Olsson, Peter LU
and Önning, Gunilla
LU
(2011)
In Genes & Nutrition
6(4).
p.429-439
- Abstract
- The understanding of how fibre-rich meals regulate molecular events at a gene level is limited. This pilot study aimed to investigate changes in gene expression in peripheral blood mononuclear cells (PBMCs) from healthy subjects after consumption of an oat bran-rich meal. Fifteen subjects (8 men and 7 women, aged 20-28 years) ingested meals with oat bran or a control meal after an overnight fast. Blood samples for analysis of postprandial glucose, insulin and triglyceride concentrations were taken during 3 h, while PBMCs for microarray gene expression profiling from five men and five women were taken before and 2 h after the meal. Analysis of transcriptome data was performed with linear mixed models to determine differentially expressed... (More)
- The understanding of how fibre-rich meals regulate molecular events at a gene level is limited. This pilot study aimed to investigate changes in gene expression in peripheral blood mononuclear cells (PBMCs) from healthy subjects after consumption of an oat bran-rich meal. Fifteen subjects (8 men and 7 women, aged 20-28 years) ingested meals with oat bran or a control meal after an overnight fast. Blood samples for analysis of postprandial glucose, insulin and triglyceride concentrations were taken during 3 h, while PBMCs for microarray gene expression profiling from five men and five women were taken before and 2 h after the meal. Analysis of transcriptome data was performed with linear mixed models to determine differentially expressed genes in response either to meal intake or meal content, and enrichment analysis was used to identify functional gene sets responding to meal intake and specifically to oat bran intake. Meal intake as such affected gene expression for genes mainly involved in metabolic stress; indicating increased inflammation due to the switch from fasting to fed state. The oat bran meal affected gene sets associated with a lower insulin level, compared with the control meal. The gene sets included genes involved in insulin secretion and β-cell development, but also protein synthesis and genes related to cancer diseases. The oat bran meal also significantly lowered postprandial blood insulin IAUC compared to control. Further studies are needed to compare these acute effects with the long-term health effects of oat bran. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2277733
- author
- Ulmius, Matilda
LU
; Johansson Persson, Anna
LU
; Krogh, Morten
LU
; Olsson, Peter
LU
and Önning, Gunilla
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Oats Microarray gene expression Peripheral mononuclear blood cells Postprandial response
- in
- Genes & Nutrition
- volume
- 6
- issue
- 4
- pages
- 429 - 439
- publisher
- BioMed Central (BMC)
- external identifiers
-
- wos:000300099500009
- scopus:81755179422
- pmid:21594609
- ISSN
- 1555-8932
- DOI
- 10.1007/s12263-011-0236-8
- language
- English
- LU publication?
- yes
- id
- ed64bdec-bc21-46f9-9216-c514c5725fd7 (old id 2277733)
- date added to LUP
- 2016-04-01 10:14:26
- date last changed
- 2024-02-21 11:42:05
@article{ed64bdec-bc21-46f9-9216-c514c5725fd7,
abstract = {{The understanding of how fibre-rich meals regulate molecular events at a gene level is limited. This pilot study aimed to investigate changes in gene expression in peripheral blood mononuclear cells (PBMCs) from healthy subjects after consumption of an oat bran-rich meal. Fifteen subjects (8 men and 7 women, aged 20-28 years) ingested meals with oat bran or a control meal after an overnight fast. Blood samples for analysis of postprandial glucose, insulin and triglyceride concentrations were taken during 3 h, while PBMCs for microarray gene expression profiling from five men and five women were taken before and 2 h after the meal. Analysis of transcriptome data was performed with linear mixed models to determine differentially expressed genes in response either to meal intake or meal content, and enrichment analysis was used to identify functional gene sets responding to meal intake and specifically to oat bran intake. Meal intake as such affected gene expression for genes mainly involved in metabolic stress; indicating increased inflammation due to the switch from fasting to fed state. The oat bran meal affected gene sets associated with a lower insulin level, compared with the control meal. The gene sets included genes involved in insulin secretion and β-cell development, but also protein synthesis and genes related to cancer diseases. The oat bran meal also significantly lowered postprandial blood insulin IAUC compared to control. Further studies are needed to compare these acute effects with the long-term health effects of oat bran.}},
author = {{Ulmius, Matilda and Johansson Persson, Anna and Krogh, Morten and Olsson, Peter and Önning, Gunilla}},
issn = {{1555-8932}},
keywords = {{Oats Microarray gene expression Peripheral mononuclear blood cells Postprandial response}},
language = {{eng}},
number = {{4}},
pages = {{429--439}},
publisher = {{BioMed Central (BMC)}},
series = {{Genes & Nutrition}},
title = {{An oat branmeal influences blood insulin levels and related gene sets in peripheral blood mononuclear cells of healthy subjects}},
url = {{http://dx.doi.org/10.1007/s12263-011-0236-8}},
doi = {{10.1007/s12263-011-0236-8}},
volume = {{6}},
year = {{2011}},
}