Gel-forming and cell-associated mucins: preparation for structural and functional studies.
(2012) In Methods in Molecular Biology 842. p.27-47- Abstract
- Secreted and transmembrane mucins are important components of innate defence at the body's mucosal surfaces. The secreted mucins are large, polymeric glycoproteins, which are largely responsible for the gel-like properties of mucus secretions. The cell-tethered mucins, however, are monomeric but are typically composed of two subunits, a larger extracellular subunit which is heavily glycosylated while the smaller more sparsely glycosylated subunit has a short extracellular region, a single-pass transmembrane domain, and a cytoplasmic tail. These two families of mucins represent high-molecular-weight glycoproteins containing serine and threonine-rich domains that are the attachment sites for large numbers of O-glycans. The high-M ( r ) and... (More)
- Secreted and transmembrane mucins are important components of innate defence at the body's mucosal surfaces. The secreted mucins are large, polymeric glycoproteins, which are largely responsible for the gel-like properties of mucus secretions. The cell-tethered mucins, however, are monomeric but are typically composed of two subunits, a larger extracellular subunit which is heavily glycosylated while the smaller more sparsely glycosylated subunit has a short extracellular region, a single-pass transmembrane domain, and a cytoplasmic tail. These two families of mucins represent high-molecular-weight glycoproteins containing serine and threonine-rich domains that are the attachment sites for large numbers of O-glycans. The high-M ( r ) and high sugar content have been exploited for the separation of mucins from the majority of components in mucus secretions. In this chapter, we describe current and well-established methods (caesium chloride density-gradient centrifugation, gel-filtration and anion-exchange chromatography, and agarose gel electrophoresis) for the extraction and purification of gel-forming and cell-surface mucins which can subsequently be used for a variety of structural and functional studies. (Less)
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https://lup.lub.lu.se/record/2336179
- author
- Davies, Julia ; Wickström, Claes LU and Thornton, David J
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods in Molecular Biology
- volume
- 842
- pages
- 27 - 47
- publisher
- Springer
- external identifiers
-
- pmid:22259128
- scopus:84856347243
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-61779-513-8_2
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Mucosal biology (013212033)
- id
- d6d4fe6d-4c15-4cc4-87dc-913100ceeed5 (old id 2336179)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22259128?dopt=Abstract
- date added to LUP
- 2016-04-04 07:03:20
- date last changed
- 2022-03-15 06:25:56
@article{d6d4fe6d-4c15-4cc4-87dc-913100ceeed5,
abstract = {{Secreted and transmembrane mucins are important components of innate defence at the body's mucosal surfaces. The secreted mucins are large, polymeric glycoproteins, which are largely responsible for the gel-like properties of mucus secretions. The cell-tethered mucins, however, are monomeric but are typically composed of two subunits, a larger extracellular subunit which is heavily glycosylated while the smaller more sparsely glycosylated subunit has a short extracellular region, a single-pass transmembrane domain, and a cytoplasmic tail. These two families of mucins represent high-molecular-weight glycoproteins containing serine and threonine-rich domains that are the attachment sites for large numbers of O-glycans. The high-M ( r ) and high sugar content have been exploited for the separation of mucins from the majority of components in mucus secretions. In this chapter, we describe current and well-established methods (caesium chloride density-gradient centrifugation, gel-filtration and anion-exchange chromatography, and agarose gel electrophoresis) for the extraction and purification of gel-forming and cell-surface mucins which can subsequently be used for a variety of structural and functional studies.}},
author = {{Davies, Julia and Wickström, Claes and Thornton, David J}},
issn = {{1940-6029}},
language = {{eng}},
pages = {{27--47}},
publisher = {{Springer}},
series = {{Methods in Molecular Biology}},
title = {{Gel-forming and cell-associated mucins: preparation for structural and functional studies.}},
url = {{http://dx.doi.org/10.1007/978-1-61779-513-8_2}},
doi = {{10.1007/978-1-61779-513-8_2}},
volume = {{842}},
year = {{2012}},
}