Overexpression of Aurora-A promotes laryngeal cancer progression by enhancing invasive ability and chromosomal instability
(2012) In European Archives of Oto-Rhino-Laryngology 269(2). p.607-614- Abstract
- The purpose of the study was to investigate the expression of Aurora-A in human laryngeal squamous cell carcinoma (LSCC) and to explore the effects of Aurora-A silencing on invasion and chromosomal instability in laryngeal cancer HEp-2 cells. The expression of Aurora-A mRNA and protein were studied using reverse transcription-PCR and Western blot in LSCC tissues and corresponding normal epithelium, respectively. In addition, the correlation between Aurora-A expression and clinicopathologic characteristics was analyzed in LSCC patients. Furthermore, HEp-2 cells were transfected with Aurora-A short hairpin RNA and the effects of knockdown of Aurora-A on tumor invasion and chromosomal instability were investigated. The results showed that... (More)
- The purpose of the study was to investigate the expression of Aurora-A in human laryngeal squamous cell carcinoma (LSCC) and to explore the effects of Aurora-A silencing on invasion and chromosomal instability in laryngeal cancer HEp-2 cells. The expression of Aurora-A mRNA and protein were studied using reverse transcription-PCR and Western blot in LSCC tissues and corresponding normal epithelium, respectively. In addition, the correlation between Aurora-A expression and clinicopathologic characteristics was analyzed in LSCC patients. Furthermore, HEp-2 cells were transfected with Aurora-A short hairpin RNA and the effects of knockdown of Aurora-A on tumor invasion and chromosomal instability were investigated. The results showed that expression of Aurora-A mRNA was significantly upregulated in laryngeal tumor tissue compared with that in normal tissue (P = 0.001), and overexpression of Aurora-A was found in 64.0% (16 of 25) of the patients by Western blotting. Upregulation of Aurora-A mRNA was significantly correlated with regional lymph node metastasis (P = 0.007) and clinical stage III/IV (P = 0.022). Overexpression of Aurora-A was significantly associated with lymph node metastasis (P = 0.027). Furthermore, disruption of Aurora-A using RNA interference technique suppressed invasive ability and chromosomal instability in HEp-2 cells. In conclusion, Aurora-A expression is elevated in human LSCC and associated with regional lymph node metastasis and late clinical stage. Overexpression of Aurora-A may contribute to LSCC carcinogenesis and progression partially due to enhancement of invasion ability and chromosomal instability. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2348805
- author
- Zhang, Hao ; Chen, Xuehua ; Jin, Yuesheng LU ; Liu, Bingya and Zhou, Liang
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aurora kinase, Laryngeal neoplasms, Cell invasion, Chromosomal, instability
- in
- European Archives of Oto-Rhino-Laryngology
- volume
- 269
- issue
- 2
- pages
- 607 - 614
- publisher
- Springer
- external identifiers
-
- wos:000299174100035
- scopus:84857064368
- pmid:21584819
- ISSN
- 0937-4477
- DOI
- 10.1007/s00405-011-1629-4
- language
- English
- LU publication?
- yes
- id
- 328d2a70-a4b5-44f3-901d-ddb0718910a9 (old id 2348805)
- date added to LUP
- 2016-04-01 11:16:08
- date last changed
- 2022-01-26 06:41:15
@article{328d2a70-a4b5-44f3-901d-ddb0718910a9,
abstract = {{The purpose of the study was to investigate the expression of Aurora-A in human laryngeal squamous cell carcinoma (LSCC) and to explore the effects of Aurora-A silencing on invasion and chromosomal instability in laryngeal cancer HEp-2 cells. The expression of Aurora-A mRNA and protein were studied using reverse transcription-PCR and Western blot in LSCC tissues and corresponding normal epithelium, respectively. In addition, the correlation between Aurora-A expression and clinicopathologic characteristics was analyzed in LSCC patients. Furthermore, HEp-2 cells were transfected with Aurora-A short hairpin RNA and the effects of knockdown of Aurora-A on tumor invasion and chromosomal instability were investigated. The results showed that expression of Aurora-A mRNA was significantly upregulated in laryngeal tumor tissue compared with that in normal tissue (P = 0.001), and overexpression of Aurora-A was found in 64.0% (16 of 25) of the patients by Western blotting. Upregulation of Aurora-A mRNA was significantly correlated with regional lymph node metastasis (P = 0.007) and clinical stage III/IV (P = 0.022). Overexpression of Aurora-A was significantly associated with lymph node metastasis (P = 0.027). Furthermore, disruption of Aurora-A using RNA interference technique suppressed invasive ability and chromosomal instability in HEp-2 cells. In conclusion, Aurora-A expression is elevated in human LSCC and associated with regional lymph node metastasis and late clinical stage. Overexpression of Aurora-A may contribute to LSCC carcinogenesis and progression partially due to enhancement of invasion ability and chromosomal instability.}},
author = {{Zhang, Hao and Chen, Xuehua and Jin, Yuesheng and Liu, Bingya and Zhou, Liang}},
issn = {{0937-4477}},
keywords = {{Aurora kinase; Laryngeal neoplasms; Cell invasion; Chromosomal; instability}},
language = {{eng}},
number = {{2}},
pages = {{607--614}},
publisher = {{Springer}},
series = {{European Archives of Oto-Rhino-Laryngology}},
title = {{Overexpression of Aurora-A promotes laryngeal cancer progression by enhancing invasive ability and chromosomal instability}},
url = {{http://dx.doi.org/10.1007/s00405-011-1629-4}},
doi = {{10.1007/s00405-011-1629-4}},
volume = {{269}},
year = {{2012}},
}