Placenta-derived extracellular vesicles : Their cargo and possible functions
Familari, Mary LU ; Cronqvist, Tina LU ; Masoumi, Zahra LU
and Hansson, Stefan
LU
(2017)
In Reproduction, Fertility and Development
29(3).
p.433-447
- Abstract
The literature on extracellular vesicles consists of rapidly expanding and often contradictory information. In this paper we attempt to review what is currently known regarding extracellular vesicles released specifically from human placental syncytiotrophoblast cells with a focus on the common but complex pregnancy-associated syndrome pre-eclampsia, where the level of syncytiotrophoblast extracellular vesicle release is significantly increased. We review common methods for syncytiotrophoblast extracellular vesicle derivation and isolation and we discuss the cargo of syncytiotrophoblast extracellular vesicles including proteins, RNA and lipids and their possible functions. A meta-analysis of available trophoblast-derived extracellular... (More)
The literature on extracellular vesicles consists of rapidly expanding and often contradictory information. In this paper we attempt to review what is currently known regarding extracellular vesicles released specifically from human placental syncytiotrophoblast cells with a focus on the common but complex pregnancy-associated syndrome pre-eclampsia, where the level of syncytiotrophoblast extracellular vesicle release is significantly increased. We review common methods for syncytiotrophoblast extracellular vesicle derivation and isolation and we discuss the cargo of syncytiotrophoblast extracellular vesicles including proteins, RNA and lipids and their possible functions. A meta-analysis of available trophoblast-derived extracellular vesicle proteomic datasets revealed only three proteins in common: albumin, fibronectin-1 and plasminogen activator inhibitor-1, suggesting some variability in vesicle cargo, most likely reflecting stage and cell type of origin. We discuss the possible sources of variability that may have led to the low number of common markers, which has led us to speculate that markers and density in common use may not be strict criteria for identifying and isolating placenta-derived exosomes.
(Less)
- author
- Familari, Mary
LU
; Cronqvist, Tina
LU
; Masoumi, Zahra
LU
and Hansson, Stefan
LU
- organization
- publishing date
- 2017
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- endothelial cells, exosomes, lipidomics, meta-analysis, proteomics, syncytiotrophoblast cells, transcriptome, trophoblast cells
- in
- Reproduction, Fertility and Development
- volume
- 29
- issue
- 3
- pages
- 15 pages
- publisher
- CSIRO Publishing
- external identifiers
-
- scopus:85013498078
- pmid:26411402
- wos:000395555500001
- ISSN
- 1031-3613
- DOI
- 10.1071/RD15143
- language
- English
- LU publication?
- yes
- id
- 2423f782-7407-483a-b3d6-f6b85168d7a1
- date added to LUP
- 2017-03-24 15:19:27
- date last changed
- 2024-03-31 04:59:35
@article{2423f782-7407-483a-b3d6-f6b85168d7a1,
abstract = {{<p>The literature on extracellular vesicles consists of rapidly expanding and often contradictory information. In this paper we attempt to review what is currently known regarding extracellular vesicles released specifically from human placental syncytiotrophoblast cells with a focus on the common but complex pregnancy-associated syndrome pre-eclampsia, where the level of syncytiotrophoblast extracellular vesicle release is significantly increased. We review common methods for syncytiotrophoblast extracellular vesicle derivation and isolation and we discuss the cargo of syncytiotrophoblast extracellular vesicles including proteins, RNA and lipids and their possible functions. A meta-analysis of available trophoblast-derived extracellular vesicle proteomic datasets revealed only three proteins in common: albumin, fibronectin-1 and plasminogen activator inhibitor-1, suggesting some variability in vesicle cargo, most likely reflecting stage and cell type of origin. We discuss the possible sources of variability that may have led to the low number of common markers, which has led us to speculate that markers and density in common use may not be strict criteria for identifying and isolating placenta-derived exosomes.</p>}},
author = {{Familari, Mary and Cronqvist, Tina and Masoumi, Zahra and Hansson, Stefan}},
issn = {{1031-3613}},
keywords = {{endothelial cells; exosomes; lipidomics; meta-analysis; proteomics; syncytiotrophoblast cells; transcriptome; trophoblast cells}},
language = {{eng}},
number = {{3}},
pages = {{433--447}},
publisher = {{CSIRO Publishing}},
series = {{Reproduction, Fertility and Development}},
title = {{Placenta-derived extracellular vesicles : Their cargo and possible functions}},
url = {{http://dx.doi.org/10.1071/RD15143}},
doi = {{10.1071/RD15143}},
volume = {{29}},
year = {{2017}},
}