Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog
(2012) In Scandinavian Journal of Clinical & Laboratory Investigation 72(2). p.175-179- Abstract
- Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 mu l microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using... (More)
- Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 mu l microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using homologue-raised antibodies when supersensitivity is required. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2515386
- author
- Rehfeld, Jens F. and Ericsson, Peter LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- peptide hormones, radioimmunoassay (RIA), homology, cholecystokinin (CCK), gastrin, Antibody cooperativity
- in
- Scandinavian Journal of Clinical & Laboratory Investigation
- volume
- 72
- issue
- 2
- pages
- 175 - 179
- publisher
- Informa Healthcare
- external identifiers
-
- wos:000301456100015
- scopus:84858423079
- pmid:22136177
- ISSN
- 1502-7686
- DOI
- 10.3109/00365513.2011.637130
- language
- English
- LU publication?
- yes
- id
- 00df49a6-89be-4c52-974e-100235ec7e03 (old id 2515386)
- date added to LUP
- 2016-04-01 13:20:14
- date last changed
- 2022-03-21 18:01:58
@article{00df49a6-89be-4c52-974e-100235ec7e03,
abstract = {{Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 mu l microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using homologue-raised antibodies when supersensitivity is required.}},
author = {{Rehfeld, Jens F. and Ericsson, Peter}},
issn = {{1502-7686}},
keywords = {{peptide hormones; radioimmunoassay (RIA); homology; cholecystokinin (CCK); gastrin; Antibody cooperativity}},
language = {{eng}},
number = {{2}},
pages = {{175--179}},
publisher = {{Informa Healthcare}},
series = {{Scandinavian Journal of Clinical & Laboratory Investigation}},
title = {{Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog}},
url = {{http://dx.doi.org/10.3109/00365513.2011.637130}},
doi = {{10.3109/00365513.2011.637130}},
volume = {{72}},
year = {{2012}},
}