Interaction of Ferrocene Mediators with Gluconobacter oxydans Immobilized Whole Cells and Membrane Fractions in Oxidation of Ethanol
(2012) In Electroanalysis 24(4). p.924-930- Abstract
- Gluconobacter oxydans whole cells and membrane fractions in combination with ferrocene mediators were used to study oxidation of ethanol. The efficiency of mediator?enzyme interaction was assessed by the ratio of maximum current to the apparent Michaelis constant (Imax/KM) for saturating mediator concentrations. The bioelectrocatalytic processes were found to be more efficient with membrane fractions. The highest Imax/KM value of 120 and 3200 mu A g/mol for, respectively, cells and fractions was obtained for ferrocene carboxylic acid. In test measurements of biological oxygen demand for rye distillers grains, the values obtained by a biosensor based on Gluconobacter membrane fractions and ferrocene were found to correlate with the... (More)
- Gluconobacter oxydans whole cells and membrane fractions in combination with ferrocene mediators were used to study oxidation of ethanol. The efficiency of mediator?enzyme interaction was assessed by the ratio of maximum current to the apparent Michaelis constant (Imax/KM) for saturating mediator concentrations. The bioelectrocatalytic processes were found to be more efficient with membrane fractions. The highest Imax/KM value of 120 and 3200 mu A g/mol for, respectively, cells and fractions was obtained for ferrocene carboxylic acid. In test measurements of biological oxygen demand for rye distillers grains, the values obtained by a biosensor based on Gluconobacter membrane fractions and ferrocene were found to correlate with the reference data. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2587623
- author
- Indzhgiya, E. Yu ; Ponamoreva, O. N. ; Alferov, V. A. ; Reshetilov, A. N. and Gorton, Lo LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bioelectrocatalysis, Ferrocene mediators, Gluconobacter oxydans, Membrane fractions
- in
- Electroanalysis
- volume
- 24
- issue
- 4
- pages
- 924 - 930
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000302160000027
- scopus:84859241227
- ISSN
- 1040-0397
- DOI
- 10.1002/elan.201100425
- language
- English
- LU publication?
- yes
- id
- 860f742f-4424-42cd-a128-f63392d9467f (old id 2587623)
- date added to LUP
- 2016-04-01 13:43:05
- date last changed
- 2023-10-15 07:10:56
@article{860f742f-4424-42cd-a128-f63392d9467f,
abstract = {{Gluconobacter oxydans whole cells and membrane fractions in combination with ferrocene mediators were used to study oxidation of ethanol. The efficiency of mediator?enzyme interaction was assessed by the ratio of maximum current to the apparent Michaelis constant (Imax/KM) for saturating mediator concentrations. The bioelectrocatalytic processes were found to be more efficient with membrane fractions. The highest Imax/KM value of 120 and 3200 mu A g/mol for, respectively, cells and fractions was obtained for ferrocene carboxylic acid. In test measurements of biological oxygen demand for rye distillers grains, the values obtained by a biosensor based on Gluconobacter membrane fractions and ferrocene were found to correlate with the reference data.}},
author = {{Indzhgiya, E. Yu and Ponamoreva, O. N. and Alferov, V. A. and Reshetilov, A. N. and Gorton, Lo}},
issn = {{1040-0397}},
keywords = {{Bioelectrocatalysis; Ferrocene mediators; Gluconobacter oxydans; Membrane fractions}},
language = {{eng}},
number = {{4}},
pages = {{924--930}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Electroanalysis}},
title = {{Interaction of Ferrocene Mediators with Gluconobacter oxydans Immobilized Whole Cells and Membrane Fractions in Oxidation of Ethanol}},
url = {{http://dx.doi.org/10.1002/elan.201100425}},
doi = {{10.1002/elan.201100425}},
volume = {{24}},
year = {{2012}},
}