Electrospun nerve guide conduits have the potential to bridge peripheral nerve injuries in vivo.
Frost, Hanna LU ; Andersson, Tomas ; Johansson, Sebastian ; Englund Johansson, Ulrica LU ; Ekström, Per LU ; Dahlin, Lars LU
and Johansson, Fredrik
LU
(2018)
In Scientific Reports
8(Article number: 16716).
- Abstract
- Electrospinning can be used to mimic the architecture of an acellular nerve graft, combining microfibers for guidance, and pores for cellular infiltration. We made electrospun nerve guides, from polycaprolactone (PCL) or poly-L-lactic acid (PLLA), with aligned fibers along the insides of the channels and random fibers around them. We bridged a 10 mm rat sciatic nerve defect with the guides, and, in selected groups, added a cell transplant derived from autologous stromal vascular fraction (SVF). For control, we compared to hollow silicone tubes; or autologous nerve grafts. PCL nerve guides had a high degree of autotomy (8/43 rats), a negative indicator with respect to future usefulness, while PLLA supported axonal regeneration, but did not... (More)
- Electrospinning can be used to mimic the architecture of an acellular nerve graft, combining microfibers for guidance, and pores for cellular infiltration. We made electrospun nerve guides, from polycaprolactone (PCL) or poly-L-lactic acid (PLLA), with aligned fibers along the insides of the channels and random fibers around them. We bridged a 10 mm rat sciatic nerve defect with the guides, and, in selected groups, added a cell transplant derived from autologous stromal vascular fraction (SVF). For control, we compared to hollow silicone tubes; or autologous nerve grafts. PCL nerve guides had a high degree of autotomy (8/43 rats), a negative indicator with respect to future usefulness, while PLLA supported axonal regeneration, but did not outperform autologous nerve grafts. Transplanted cells survived in the PLLA nerve guides, but axonal regeneration was not enhanced as compared to nerve guides alone. The inflammatory response was partially enhanced by the transplanted cells in PLLA nerve grafts; Schwann cells were poorly distributed compared to nerve guide without cells. Tailor-made electrospun nerve guides support axonal regeneration in vivo, and can act as vehicles for co-transplanted cells. Our results motivate further studies exploring novel nerve guides and the effect of stromal cell-derived factors on nerve generation. (Less)
- Abstract (Swedish)
- Electrospinning can be used to mimic the architecture of an acellular nerve graft, combining microfibers for guidance, and pores for cellular infiltration. We made electrospun nerve guides, from polycaprolactone (PCL) or poly-L-lactic acid (PLLA), with aligned fibers along the insides of the channels and random fibers around them. We bridged a 10 mm rat sciatic nerve defect with the guides, and, in selected groups, added a cell transplant derived from autologous stromal vascular fraction (SVF). For control, we compared to hollow silicone tubes; or autologous nerve grafts. PCL nerve guides had a high degree of autotomy (8/43 rats), a negative indicator with respect to future usefulness, while PLLA supported axonal regeneration, but did not... (More)
- Electrospinning can be used to mimic the architecture of an acellular nerve graft, combining microfibers for guidance, and pores for cellular infiltration. We made electrospun nerve guides, from polycaprolactone (PCL) or poly-L-lactic acid (PLLA), with aligned fibers along the insides of the channels and random fibers around them. We bridged a 10 mm rat sciatic nerve defect with the guides, and, in selected groups, added a cell transplant derived from autologous stromal vascular fraction (SVF). For control, we compared to hollow silicone tubes; or autologous nerve grafts. PCL nerve guides had a high degree of autotomy (8/43 rats), a negative indicator with respect to future usefulness, while PLLA supported axonal regeneration, but did not outperform autologous nerve grafts. Transplanted cells survived in the PLLA nerve guides, but axonal regeneration was not enhanced as compared to nerve guides alone. The inflammatory response was partially enhanced by the transplanted cells in PLLA nerve grafts; Schwann cells were poorly distributed compared to nerve guide without cells. Tailor-made electrospun nerve guides support axonal regeneration in vivo, and can act as vehicles for co-transplanted cells. Our results motivate further studies exploring novel nerve guides and the effect of stromal cell-derived factors on nerve generation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2621120a-00b7-428e-b844-139741c2a97b
- author
- Frost, Hanna
LU
; Andersson, Tomas
; Johansson, Sebastian
; Englund Johansson, Ulrica
LU
; Ekström, Per
LU
; Dahlin, Lars
LU
and Johansson, Fredrik
LU
- organization
- publishing date
- 2018-11-14
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- peripheral nerve injury, nerve repair, electrospinning, nanofibers, cell therapy, autologous transplantation
- in
- Scientific Reports
- volume
- 8
- issue
- Article number: 16716
- article number
- 16716
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85056484486
- pmid:30425260
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-018-34699-8
- language
- English
- LU publication?
- yes
- id
- 2621120a-00b7-428e-b844-139741c2a97b
- date added to LUP
- 2018-11-15 11:32:25
- date last changed
- 2023-11-18 05:36:30
@article{2621120a-00b7-428e-b844-139741c2a97b,
abstract = {{Electrospinning can be used to mimic the architecture of an acellular nerve graft, combining microfibers for guidance, and pores for cellular infiltration. We made electrospun nerve guides, from polycaprolactone (PCL) or poly-L-lactic acid (PLLA), with aligned fibers along the insides of the channels and random fibers around them. We bridged a 10 mm rat sciatic nerve defect with the guides, and, in selected groups, added a cell transplant derived from autologous stromal vascular fraction (SVF). For control, we compared to hollow silicone tubes; or autologous nerve grafts. PCL nerve guides had a high degree of autotomy (8/43 rats), a negative indicator with respect to future usefulness, while PLLA supported axonal regeneration, but did not outperform autologous nerve grafts. Transplanted cells survived in the PLLA nerve guides, but axonal regeneration was not enhanced as compared to nerve guides alone. The inflammatory response was partially enhanced by the transplanted cells in PLLA nerve grafts; Schwann cells were poorly distributed compared to nerve guide without cells. Tailor-made electrospun nerve guides support axonal regeneration in vivo, and can act as vehicles for co-transplanted cells. Our results motivate further studies exploring novel nerve guides and the effect of stromal cell-derived factors on nerve generation.}},
author = {{Frost, Hanna and Andersson, Tomas and Johansson, Sebastian and Englund Johansson, Ulrica and Ekström, Per and Dahlin, Lars and Johansson, Fredrik}},
issn = {{2045-2322}},
keywords = {{peripheral nerve injury; nerve repair; electrospinning; nanofibers; cell therapy; autologous transplantation}},
language = {{eng}},
month = {{11}},
number = {{Article number: 16716}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Electrospun nerve guide conduits have the potential to bridge peripheral nerve injuries in vivo.}},
url = {{http://dx.doi.org/10.1038/s41598-018-34699-8}},
doi = {{10.1038/s41598-018-34699-8}},
volume = {{8}},
year = {{2018}},
}