Thiol metabolism in preterm infants during the first week of life
Ahola, T ; Levonen, AL ; Fellman, Vineta LU
and Lapatto, R
(2004)
In Scandinavian Journal of Clinical & Laboratory Investigation
64(7).
p.649-658
- Abstract
- Background: Oxidative stress is implicated in the pathogenesis of several complications of prematurity. The glutathione cycle is one of the most important intracellular antioxidant systems. The synthesis of glutathione may not be adequate in preterm neonates because of the low levels of cysteine available. The aim of this study was to evaluate cysteine and glutathione metabolism during the first week of life in preterm infants. Methods: Plasma and erythrocyte thiol concentrations were measured in 78 preterm infants with a birthweight of 500 1500 g, and erythrocyte glutamate-cysteine ligase (GCL), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferases (GST) and glucose 6-phosphatedehydrogenase (G6PDH) in 26... (More)
- Background: Oxidative stress is implicated in the pathogenesis of several complications of prematurity. The glutathione cycle is one of the most important intracellular antioxidant systems. The synthesis of glutathione may not be adequate in preterm neonates because of the low levels of cysteine available. The aim of this study was to evaluate cysteine and glutathione metabolism during the first week of life in preterm infants. Methods: Plasma and erythrocyte thiol concentrations were measured in 78 preterm infants with a birthweight of 500 1500 g, and erythrocyte glutamate-cysteine ligase (GCL), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferases (GST) and glucose 6-phosphatedehydrogenase (G6PDH) in 26 infants with a birthweight of 1000 - 1500 g. Results: The mean (SD) plasma glutathione concentration increased from day 0 to day 1 (14.9 (7.1) vs. 27.7 (11.9) mumol/L, p<0.001), and then decreased. The plasma cysteine concentration changed in the opposite direction ( 172 (59) vs. 129 (42) μmol/L, p<0.01). In infants with respiratory distress syndrome (RDS) the mean plasma glutathione concentration, but not cysteine, was lower on day 0 compared with infants without RDS (11.7 (5.2) vs. 21.4 (5.6) mumol/L, p<0.01). Erythrocyte glutathione concentration decreased during the first week of life, whereas erythrocyte cysteine concentration increased significantly from day 3 to day 7 (p<0.01). Erythrocyte cysteine and glutathione concentrations had a positive correlation. The GCL and GR activities did not change, but GST and G6PDH activities decreased during the first week (p<0.01). GPx activity decreased until day 3 (p<0.01) and was higher on day 0 and day 1 in infants with RDS. Conclusions: Very low birthweight infants have an initial increase in plasma glutathione and initial decrease in plasma cysteine level during the first week of life, and also a positive correlation between erythrocyte cysteine and glutathione levels. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/264441
- author
- Ahola, T
; Levonen, AL
; Fellman, Vineta
LU
and Lapatto, R
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- glutathione peroxidase, glutathione, glutamate-cysteine ligase, glucose 6-phosphatedehydrogenase, bronchopulmonary dysplasia, cysteine, glutathione reductase, glutathione transferase, respiratory distress, syndrome
- in
- Scandinavian Journal of Clinical & Laboratory Investigation
- volume
- 64
- issue
- 7
- pages
- 649 - 658
- publisher
- Informa Healthcare
- external identifiers
-
- pmid:15513322
- wos:000224328400006
- scopus:7044222381
- pmid:15513322
- ISSN
- 1502-7686
- DOI
- 10.1080/00365510410002959
- language
- English
- LU publication?
- yes
- id
- 7cb1bb8d-1f32-467f-b531-70ac7b8b9411 (old id 264441)
- date added to LUP
- 2016-04-01 16:15:17
- date last changed
- 2022-01-28 18:23:10
@article{7cb1bb8d-1f32-467f-b531-70ac7b8b9411,
abstract = {{Background: Oxidative stress is implicated in the pathogenesis of several complications of prematurity. The glutathione cycle is one of the most important intracellular antioxidant systems. The synthesis of glutathione may not be adequate in preterm neonates because of the low levels of cysteine available. The aim of this study was to evaluate cysteine and glutathione metabolism during the first week of life in preterm infants. Methods: Plasma and erythrocyte thiol concentrations were measured in 78 preterm infants with a birthweight of 500 1500 g, and erythrocyte glutamate-cysteine ligase (GCL), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferases (GST) and glucose 6-phosphatedehydrogenase (G6PDH) in 26 infants with a birthweight of 1000 - 1500 g. Results: The mean (SD) plasma glutathione concentration increased from day 0 to day 1 (14.9 (7.1) vs. 27.7 (11.9) mumol/L, p<0.001), and then decreased. The plasma cysteine concentration changed in the opposite direction ( 172 (59) vs. 129 (42) μmol/L, p<0.01). In infants with respiratory distress syndrome (RDS) the mean plasma glutathione concentration, but not cysteine, was lower on day 0 compared with infants without RDS (11.7 (5.2) vs. 21.4 (5.6) mumol/L, p<0.01). Erythrocyte glutathione concentration decreased during the first week of life, whereas erythrocyte cysteine concentration increased significantly from day 3 to day 7 (p<0.01). Erythrocyte cysteine and glutathione concentrations had a positive correlation. The GCL and GR activities did not change, but GST and G6PDH activities decreased during the first week (p<0.01). GPx activity decreased until day 3 (p<0.01) and was higher on day 0 and day 1 in infants with RDS. Conclusions: Very low birthweight infants have an initial increase in plasma glutathione and initial decrease in plasma cysteine level during the first week of life, and also a positive correlation between erythrocyte cysteine and glutathione levels.}},
author = {{Ahola, T and Levonen, AL and Fellman, Vineta and Lapatto, R}},
issn = {{1502-7686}},
keywords = {{glutathione peroxidase; glutathione; glutamate-cysteine ligase; glucose 6-phosphatedehydrogenase; bronchopulmonary dysplasia; cysteine; glutathione reductase; glutathione transferase; respiratory distress; syndrome}},
language = {{eng}},
number = {{7}},
pages = {{649--658}},
publisher = {{Informa Healthcare}},
series = {{Scandinavian Journal of Clinical & Laboratory Investigation}},
title = {{Thiol metabolism in preterm infants during the first week of life}},
url = {{http://dx.doi.org/10.1080/00365510410002959}},
doi = {{10.1080/00365510410002959}},
volume = {{64}},
year = {{2004}},
}