Retinoblastoma protein in human renal cell carcinoma in relation to alterations in G1/S regulatory proteins
(2004) In International Journal of Cancer 109(2). p.189-193- Abstract
- The retinoblastoma gene product (pRb) is the main substrate for cyclin-dependent kinases (CDKs) during the G1/S transition. Aberrations in cell cycle regulatory proteins, which have been observed in many malignancies, can theoretically cause increased phosphorylation of pRb due to unbalanced CDK activities. The expression and phosphorylation of pRb and potential associations to cell cycle aberrations in renal cell carcinomas (RCC) has only partly been clarified. We therefore evaluated the presence of pRb and the level of pRb-phosphorylation in 216 RCCs arranged in tissue microarrays by using different pRb-antibodies, including pRb-phosphospecific antibodies. Most RCCs (95%) expressed pRb, while cases with the low pRb levels, potentially... (More)
- The retinoblastoma gene product (pRb) is the main substrate for cyclin-dependent kinases (CDKs) during the G1/S transition. Aberrations in cell cycle regulatory proteins, which have been observed in many malignancies, can theoretically cause increased phosphorylation of pRb due to unbalanced CDK activities. The expression and phosphorylation of pRb and potential associations to cell cycle aberrations in renal cell carcinomas (RCC) has only partly been clarified. We therefore evaluated the presence of pRb and the level of pRb-phosphorylation in 216 RCCs arranged in tissue microarrays by using different pRb-antibodies, including pRb-phosphospecific antibodies. Most RCCs (95%) expressed pRb, while cases with the low pRb levels, potentially indicative for pRb-inactivation, were few. In order to detect secondary alterations to a potential pRb-inactivation, the p 16 expression was also monitored. None of the tumors exhibited increased p 16 levels, confirming that pRb-inactivation is rare in RCC. Phosphorylated pRb was detected in approximately 50% of the RCCs, using Western blotting or immunohistochemistry. The immunohistochemical ppRb(ser807/811) levels were associated with high proliferation, cyclin D1, cyclin E and p27 protein content. Surprisingly, there was no association between pRb-phosphorylation and clinicopathological data. In summary, pRb seemed to be functional and aberrations in G1/S-regulatory proteins were associated with increased phosphorylation of pRb and proliferation. The data supports that pRb might be one of the main cell cycle regulators in RCC. (C) 2003 Wiley-Liss, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/287108
- author
- Hedberg, Y ; Ljungberg, B ; Roos, G and Landberg, Göran LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- renal cell carcinoma, GI/S-cyclins, cell cycle, retinoblastoma protein, phosphorylation
- in
- International Journal of Cancer
- volume
- 109
- issue
- 2
- pages
- 189 - 193
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000189020300006
- pmid:14750168
- scopus:1242342071
- ISSN
- 0020-7136
- DOI
- 10.1002/ijc.11665
- language
- English
- LU publication?
- yes
- id
- d45c093e-3548-4a3b-9098-9cbd42684774 (old id 287108)
- date added to LUP
- 2016-04-01 11:42:59
- date last changed
- 2022-02-18 03:51:12
@article{d45c093e-3548-4a3b-9098-9cbd42684774,
abstract = {{The retinoblastoma gene product (pRb) is the main substrate for cyclin-dependent kinases (CDKs) during the G1/S transition. Aberrations in cell cycle regulatory proteins, which have been observed in many malignancies, can theoretically cause increased phosphorylation of pRb due to unbalanced CDK activities. The expression and phosphorylation of pRb and potential associations to cell cycle aberrations in renal cell carcinomas (RCC) has only partly been clarified. We therefore evaluated the presence of pRb and the level of pRb-phosphorylation in 216 RCCs arranged in tissue microarrays by using different pRb-antibodies, including pRb-phosphospecific antibodies. Most RCCs (95%) expressed pRb, while cases with the low pRb levels, potentially indicative for pRb-inactivation, were few. In order to detect secondary alterations to a potential pRb-inactivation, the p 16 expression was also monitored. None of the tumors exhibited increased p 16 levels, confirming that pRb-inactivation is rare in RCC. Phosphorylated pRb was detected in approximately 50% of the RCCs, using Western blotting or immunohistochemistry. The immunohistochemical ppRb(ser807/811) levels were associated with high proliferation, cyclin D1, cyclin E and p27 protein content. Surprisingly, there was no association between pRb-phosphorylation and clinicopathological data. In summary, pRb seemed to be functional and aberrations in G1/S-regulatory proteins were associated with increased phosphorylation of pRb and proliferation. The data supports that pRb might be one of the main cell cycle regulators in RCC. (C) 2003 Wiley-Liss, Inc.}},
author = {{Hedberg, Y and Ljungberg, B and Roos, G and Landberg, Göran}},
issn = {{0020-7136}},
keywords = {{renal cell carcinoma; GI/S-cyclins; cell cycle; retinoblastoma protein; phosphorylation}},
language = {{eng}},
number = {{2}},
pages = {{189--193}},
publisher = {{John Wiley & Sons Inc.}},
series = {{International Journal of Cancer}},
title = {{Retinoblastoma protein in human renal cell carcinoma in relation to alterations in G1/S regulatory proteins}},
url = {{http://dx.doi.org/10.1002/ijc.11665}},
doi = {{10.1002/ijc.11665}},
volume = {{109}},
year = {{2004}},
}