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Escherichia coli RNase E and RNase G cleave a Bacillus subtilis transcript at the same site in a structure-dependent manner

Hambraeus, Gustav LU and Rutberg, Blanka LU (2004) In Archives of Microbiology 181(2). p.137-143
Abstract
The decay of Bacillus subtilis aprE leader-lacZ mRNA was examined in Escherichia coli wild-type and in mutants deficient in RNase E, RNase G, or both. Two versions of the mRNA were studied: the wild-type mRNA, which has a stem-loop at the 5' end, and a mutant mRNA, with a single-stranded 5' end. The half-life of both transcripts was determined by RNase E, the half-life of the mutant transcript being one-third of that of the wild-type transcript. RNase G cleaved both transcripts at a site within an AU-rich sequence in the stem-loop region, but cleavage was much more efficient when the stem-loop was destabilized. RNase E cleaved at the same site, but less efficiently and only in the mutant transcript.
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
mRNA degradation, RNase E, RNase G
in
Archives of Microbiology
volume
181
issue
2
pages
137 - 143
publisher
Springer
external identifiers
  • wos:000188457000007
  • pmid:14685649
  • scopus:1242337413
ISSN
0302-8933
DOI
10.1007/s00203-003-0637-1
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Cell and Organism Biology (Closed 2011.) (011002100)
id
152e0a3f-f54d-43cc-97ef-2ba28411a9ca (old id 289577)
date added to LUP
2016-04-01 16:46:23
date last changed
2022-01-28 22:01:24
@article{152e0a3f-f54d-43cc-97ef-2ba28411a9ca,
  abstract     = {{The decay of Bacillus subtilis aprE leader-lacZ mRNA was examined in Escherichia coli wild-type and in mutants deficient in RNase E, RNase G, or both. Two versions of the mRNA were studied: the wild-type mRNA, which has a stem-loop at the 5' end, and a mutant mRNA, with a single-stranded 5' end. The half-life of both transcripts was determined by RNase E, the half-life of the mutant transcript being one-third of that of the wild-type transcript. RNase G cleaved both transcripts at a site within an AU-rich sequence in the stem-loop region, but cleavage was much more efficient when the stem-loop was destabilized. RNase E cleaved at the same site, but less efficiently and only in the mutant transcript.}},
  author       = {{Hambraeus, Gustav and Rutberg, Blanka}},
  issn         = {{0302-8933}},
  keywords     = {{mRNA degradation; RNase E; RNase G}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{137--143}},
  publisher    = {{Springer}},
  series       = {{Archives of Microbiology}},
  title        = {{Escherichia coli RNase E and RNase G cleave a Bacillus subtilis transcript at the same site in a structure-dependent manner}},
  url          = {{http://dx.doi.org/10.1007/s00203-003-0637-1}},
  doi          = {{10.1007/s00203-003-0637-1}},
  volume       = {{181}},
  year         = {{2004}},
}