Hydrodynamic radius determination with asymmetrical flow field-flow fractionation using decaying cross-flows. Part II. Experimental evaluation.
(2012) In Journal of chromatography. A 1253. p.127-133- Abstract
- In this study we investigate the effect of programmed cross-flows on the error in the hydrodynamic radii (r(h)) determination with asymmetrical flow field-flow fractionation (AsFlFFF). Three different standard polystyrene particles (nominal radii of 30 and 40 and 50nm) are fractionated with exponentially and linearly decaying cross-flows with different decay rates. Hydrodynamic radii are calculated according to retention theory including steric effects. Rapid decay is expected to give rise to systematic deviations in r(h) determination. The error in r(h) was found to be small when decay rates with half-lives longer than 6min were used, whereas steeper decays could give rise to errors as high as 16% of the particle size. The error is often... (More)
- In this study we investigate the effect of programmed cross-flows on the error in the hydrodynamic radii (r(h)) determination with asymmetrical flow field-flow fractionation (AsFlFFF). Three different standard polystyrene particles (nominal radii of 30 and 40 and 50nm) are fractionated with exponentially and linearly decaying cross-flows with different decay rates. Hydrodynamic radii are calculated according to retention theory including steric effects. Rapid decay is expected to give rise to systematic deviations in r(h) determination. The error in r(h) was found to be small when decay rates with half-lives longer than 6min were used, whereas steeper decays could give rise to errors as high as 16% of the particle size. The error is often explained in terms of secondary relaxation. However, comparisons show that experimental errors are significantly larger than what would be expected due to secondary relaxation, suggesting that other factors also have to be considered in order to fully understand deviations for rapidly decaying cross-flow. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2966839
- author
- Magnusson, Emma LU ; Håkansson, Andreas LU ; Janiak, John LU ; Bergenståhl, Björn LU and Nilsson, Lars LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of chromatography. A
- volume
- 1253
- pages
- 127 - 133
- publisher
- Elsevier
- external identifiers
-
- wos:000307793700015
- pmid:22818771
- scopus:84864570841
- pmid:22818771
- ISSN
- 1873-3778
- DOI
- 10.1016/j.chroma.2012.07.005
- language
- English
- LU publication?
- yes
- id
- 81071597-96d3-457c-a366-fd8aa1e46d9f (old id 2966839)
- date added to LUP
- 2016-04-01 13:53:50
- date last changed
- 2023-11-12 23:32:23
@article{81071597-96d3-457c-a366-fd8aa1e46d9f,
abstract = {{In this study we investigate the effect of programmed cross-flows on the error in the hydrodynamic radii (r(h)) determination with asymmetrical flow field-flow fractionation (AsFlFFF). Three different standard polystyrene particles (nominal radii of 30 and 40 and 50nm) are fractionated with exponentially and linearly decaying cross-flows with different decay rates. Hydrodynamic radii are calculated according to retention theory including steric effects. Rapid decay is expected to give rise to systematic deviations in r(h) determination. The error in r(h) was found to be small when decay rates with half-lives longer than 6min were used, whereas steeper decays could give rise to errors as high as 16% of the particle size. The error is often explained in terms of secondary relaxation. However, comparisons show that experimental errors are significantly larger than what would be expected due to secondary relaxation, suggesting that other factors also have to be considered in order to fully understand deviations for rapidly decaying cross-flow.}},
author = {{Magnusson, Emma and Håkansson, Andreas and Janiak, John and Bergenståhl, Björn and Nilsson, Lars}},
issn = {{1873-3778}},
language = {{eng}},
pages = {{127--133}},
publisher = {{Elsevier}},
series = {{Journal of chromatography. A}},
title = {{Hydrodynamic radius determination with asymmetrical flow field-flow fractionation using decaying cross-flows. Part II. Experimental evaluation.}},
url = {{http://dx.doi.org/10.1016/j.chroma.2012.07.005}},
doi = {{10.1016/j.chroma.2012.07.005}},
volume = {{1253}},
year = {{2012}},
}