Characterization of the receptor response for the neuropeptide Y-evoked suppression of parasympathetically-mediated contractions in the guinea pig trachea
(1997) In Regulatory Peptides 71(2). p.97-101- Abstract
Neuropeptide Y (NPY) acts via several distinct receptor types. The aim of the present study was to examine which NPY receptors are coupled to inhibition of parasympathetically-mediated contractions of the isolated guinea pig trachea. Electrical field stimulation of tracheal rings evoked a rapid twitch, which was abolished by atropine (1 microM). NPY, the structurally related hormone peptide YY (PYY), the Y2 receptor agonist [Cys2, Aoc5-24, D-Cys27]NPY, as well as NPY 5-36 and NPY 13-36 evoked a concentration-dependent inhibition of the electrically-stimulated twitches. Pretreatment with the Y1 receptor-selective antagonist BIBP3226 (1 microM) failed to prevent the NPY-induced inhibition. Although less potent than NPY, the Y1 (and Y4-Y6)... (More)
Neuropeptide Y (NPY) acts via several distinct receptor types. The aim of the present study was to examine which NPY receptors are coupled to inhibition of parasympathetically-mediated contractions of the isolated guinea pig trachea. Electrical field stimulation of tracheal rings evoked a rapid twitch, which was abolished by atropine (1 microM). NPY, the structurally related hormone peptide YY (PYY), the Y2 receptor agonist [Cys2, Aoc5-24, D-Cys27]NPY, as well as NPY 5-36 and NPY 13-36 evoked a concentration-dependent inhibition of the electrically-stimulated twitches. Pretreatment with the Y1 receptor-selective antagonist BIBP3226 (1 microM) failed to prevent the NPY-induced inhibition. Although less potent than NPY, the Y1 (and Y4-Y6) receptor agonist [Leu31, Pro34]NPY also inhibited the electrically-stimulated twitches. Another NPY-related peptide, pancreatic polypeptide, which recognizes Y4-Y6 receptors did not affect the stimulated twitches at concentrations up to 1 microM. However, pretreatment with the Y1 receptor-selective antagonist BIBP3226 (1 microM) virtually abolished the inhibition evoked by [Leu31, Pro34]NPY. None of the peptides affected the baseline tension and BIBP3226 (1 microM) per se did not affect the amplitude of the electrically-stimulated twitches. In conclusion, it seems that NPY and PYY are capable of suppressing parasympathetically mediated contractions in the guinea pig trachea mainly via Y2 receptors, but there is also a small contribution from Y1 receptors.
(Less)
- author
- Grundemar, L LU
- publishing date
- 1997-08-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Arginine/analogs & derivatives, Atropine/pharmacology, Dose-Response Relationship, Drug, Electric Stimulation, Guinea Pigs, In Vitro Techniques, Muscle Contraction/drug effects, Muscle, Smooth/drug effects, Neuropeptide Y/analogs & derivatives, Pancreatic Polypeptide/pharmacology, Peptide Fragments/pharmacology, Peptide YY/pharmacology, Receptors, Neuropeptide Y/antagonists & inhibitors, Trachea/innervation
- in
- Regulatory Peptides
- volume
- 71
- issue
- 2
- pages
- 97 - 101
- publisher
- Elsevier
- external identifiers
-
- scopus:0030733663
- pmid:9416991
- ISSN
- 0167-0115
- DOI
- 10.1016/s0167-0115(97)01023-9
- language
- English
- LU publication?
- no
- id
- 2bd232ec-8c72-426f-9c6b-eaacaba56cea
- date added to LUP
- 2019-09-03 13:55:51
- date last changed
- 2024-01-01 18:33:59
@article{2bd232ec-8c72-426f-9c6b-eaacaba56cea,
abstract = {{<p>Neuropeptide Y (NPY) acts via several distinct receptor types. The aim of the present study was to examine which NPY receptors are coupled to inhibition of parasympathetically-mediated contractions of the isolated guinea pig trachea. Electrical field stimulation of tracheal rings evoked a rapid twitch, which was abolished by atropine (1 microM). NPY, the structurally related hormone peptide YY (PYY), the Y2 receptor agonist [Cys2, Aoc5-24, D-Cys27]NPY, as well as NPY 5-36 and NPY 13-36 evoked a concentration-dependent inhibition of the electrically-stimulated twitches. Pretreatment with the Y1 receptor-selective antagonist BIBP3226 (1 microM) failed to prevent the NPY-induced inhibition. Although less potent than NPY, the Y1 (and Y4-Y6) receptor agonist [Leu31, Pro34]NPY also inhibited the electrically-stimulated twitches. Another NPY-related peptide, pancreatic polypeptide, which recognizes Y4-Y6 receptors did not affect the stimulated twitches at concentrations up to 1 microM. However, pretreatment with the Y1 receptor-selective antagonist BIBP3226 (1 microM) virtually abolished the inhibition evoked by [Leu31, Pro34]NPY. None of the peptides affected the baseline tension and BIBP3226 (1 microM) per se did not affect the amplitude of the electrically-stimulated twitches. In conclusion, it seems that NPY and PYY are capable of suppressing parasympathetically mediated contractions in the guinea pig trachea mainly via Y2 receptors, but there is also a small contribution from Y1 receptors.</p>}},
author = {{Grundemar, L}},
issn = {{0167-0115}},
keywords = {{Animals; Arginine/analogs & derivatives; Atropine/pharmacology; Dose-Response Relationship, Drug; Electric Stimulation; Guinea Pigs; In Vitro Techniques; Muscle Contraction/drug effects; Muscle, Smooth/drug effects; Neuropeptide Y/analogs & derivatives; Pancreatic Polypeptide/pharmacology; Peptide Fragments/pharmacology; Peptide YY/pharmacology; Receptors, Neuropeptide Y/antagonists & inhibitors; Trachea/innervation}},
language = {{eng}},
month = {{08}},
number = {{2}},
pages = {{97--101}},
publisher = {{Elsevier}},
series = {{Regulatory Peptides}},
title = {{Characterization of the receptor response for the neuropeptide Y-evoked suppression of parasympathetically-mediated contractions in the guinea pig trachea}},
url = {{http://dx.doi.org/10.1016/s0167-0115(97)01023-9}},
doi = {{10.1016/s0167-0115(97)01023-9}},
volume = {{71}},
year = {{1997}},
}