Stimulation of insulin release by thiols
Hellman, Bo ; Idahl, Lars Åke ; Lernmark, Åke LU
; Sehlin, Janove
and Täljedal, Inge Bert
(1975)
In BBA - General Subjects
392(1).
p.101-109
- Abstract
The effects of thiol compounds on insulin release were studied in microdissected pancreatic islets of non-inbred ob/ob mice. In control experiments the reactivity of thiols against 6,6′-dithiodinicotinic acid and the degradation of mouse insulin were measured. At a concentration of 0.1 mM, 1-thio-D-glucose or reduced glutathione potentiated the insulin-releasing action of 10 mM D-glucose without affecting glucose oxidation. When tested at a concentration equivalent to about 0.1 mM reactive thiol, dextran-linked L-cysteine also potentiated the glucose-induced insulin secretion. In microperifusion experiments the insulin-releasing action of 1-thio-D-glucose was found to exhibit a rapid onset followed by a decline of the secretory rate to... (More)
The effects of thiol compounds on insulin release were studied in microdissected pancreatic islets of non-inbred ob/ob mice. In control experiments the reactivity of thiols against 6,6′-dithiodinicotinic acid and the degradation of mouse insulin were measured. At a concentration of 0.1 mM, 1-thio-D-glucose or reduced glutathione potentiated the insulin-releasing action of 10 mM D-glucose without affecting glucose oxidation. When tested at a concentration equivalent to about 0.1 mM reactive thiol, dextran-linked L-cysteine also potentiated the glucose-induced insulin secretion. In microperifusion experiments the insulin-releasing action of 1-thio-D-glucose was found to exhibit a rapid onset followed by a decline of the secretory rate to values lower than those observed with 10 mM D-glucose alone. No thiol stimulated insulin release in the absence of glucose. It is suggested that thiol compounds stimulate insulin release by splitting membrane disulphides in the β-cells.
(Less)
- author
- Hellman, Bo
; Idahl, Lars Åke
; Lernmark, Åke
LU
; Sehlin, Janove
and Täljedal, Inge Bert
- publishing date
- 1975-05-05
- type
- Contribution to journal
- publication status
- published
- in
- BBA - General Subjects
- volume
- 392
- issue
- 1
- pages
- 101 - 109
- publisher
- Elsevier
- external identifiers
-
- scopus:0016738060
- pmid:1092370
- ISSN
- 0304-4165
- DOI
- 10.1016/0304-4165(75)90170-1
- language
- English
- LU publication?
- no
- id
- 2f00b47c-5e25-4f89-b7e6-c75b94641fdc
- date added to LUP
- 2019-09-18 12:14:25
- date last changed
- 2024-03-13 08:24:14
@article{2f00b47c-5e25-4f89-b7e6-c75b94641fdc,
abstract = {{<p>The effects of thiol compounds on insulin release were studied in microdissected pancreatic islets of non-inbred ob/ob mice. In control experiments the reactivity of thiols against 6,6′-dithiodinicotinic acid and the degradation of mouse insulin were measured. At a concentration of 0.1 mM, 1-thio-D-glucose or reduced glutathione potentiated the insulin-releasing action of 10 mM D-glucose without affecting glucose oxidation. When tested at a concentration equivalent to about 0.1 mM reactive thiol, dextran-linked L-cysteine also potentiated the glucose-induced insulin secretion. In microperifusion experiments the insulin-releasing action of 1-thio-D-glucose was found to exhibit a rapid onset followed by a decline of the secretory rate to values lower than those observed with 10 mM D-glucose alone. No thiol stimulated insulin release in the absence of glucose. It is suggested that thiol compounds stimulate insulin release by splitting membrane disulphides in the β-cells.</p>}},
author = {{Hellman, Bo and Idahl, Lars Åke and Lernmark, Åke and Sehlin, Janove and Täljedal, Inge Bert}},
issn = {{0304-4165}},
language = {{eng}},
month = {{05}},
number = {{1}},
pages = {{101--109}},
publisher = {{Elsevier}},
series = {{BBA - General Subjects}},
title = {{Stimulation of insulin release by thiols}},
url = {{http://dx.doi.org/10.1016/0304-4165(75)90170-1}},
doi = {{10.1016/0304-4165(75)90170-1}},
volume = {{392}},
year = {{1975}},
}