Vaginal self-sampling without preservative for human papillomavirus testing shows good sensitivity.
(2013) In Journal of Clinical Virology 56(1). p.52-56- Abstract
- BACKGROUND:
Several strategies have been used to reach non-attending women in organized cervical-cancer-screening programs, with varying success. Self-sampling (SS) for HPV is effective for increasing coverage in screening programs, but requires expensive commercial sampling kits.
OBJECTIVE:
We aimed to evaluate if vaginal SS, without commercial preservatives was adequate for HPV testing.
STUDY DESIGN:
Women with abnormal cervical smears as determined from the organized screening program were invited to a colposcopy clinic. The 121 women were asked to insert a cotton swab into the vagina and rotate it, put the cotton swab into a sterile cryotube, break the upper part of... (More) - BACKGROUND:
Several strategies have been used to reach non-attending women in organized cervical-cancer-screening programs, with varying success. Self-sampling (SS) for HPV is effective for increasing coverage in screening programs, but requires expensive commercial sampling kits.
OBJECTIVE:
We aimed to evaluate if vaginal SS, without commercial preservatives was adequate for HPV testing.
STUDY DESIGN:
Women with abnormal cervical smears as determined from the organized screening program were invited to a colposcopy clinic. The 121 women were asked to insert a cotton swab into the vagina and rotate it, put the cotton swab into a sterile cryotube, break the upper part of the stick and put the cap on. Thereafter, the gynaecologist collected a liquid based cytology (LBC) sample. The presence of HPV-types in SS and LBC samples was analysed with PCR and luminex-based typing.
RESULTS:
High-risk-HPV (hr-HPV) DNA was found in 65 of the tested 108 SS (60%; 95% CI 0.50-0.69), whereas LBC found hr-HPV in 64/108 samples (59%; 95% CI 0.49-0.69). The agreement between sampling with SS and LBC was good, kappa value 0.67 (95% CI; 0.53-0.81). The sensitivity for SS with hr-HPV to find HSIL was 81% (95% CI; 67-95%), specificity 49% (95% CI; 37-60%) and the sensitivity for LBC with hr-HPV to find HSIL was 90% (95% CI 80-100%), specificity53% (95% CI; 42-65%).
CONCLUSIONS:
This new vaginal self-sampling method detects hr-HPV-infections with similar sensitivity as a cervical smear taken by a gynaecologist. This self-sampling method is cost-effective and well tolerated, and the kit is suitable for regular mail transport. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3123577
- author
- Darlin, Lotten LU ; Borgfeldt, Christer LU ; Forslund, Ola LU ; Henic, Emir LU ; Dillner, Joakim LU and Kannisto, Päivi LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Clinical Virology
- volume
- 56
- issue
- 1
- pages
- 52 - 56
- publisher
- Elsevier
- external identifiers
-
- wos:000312885600011
- pmid:23017435
- scopus:84871475409
- pmid:23017435
- ISSN
- 1386-6532
- DOI
- 10.1016/j.jcv.2012.09.002
- language
- English
- LU publication?
- yes
- id
- 0e766020-02f2-43a1-b7a7-29ae0ed73db9 (old id 3123577)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23017435?dopt=Abstract
- date added to LUP
- 2016-04-04 07:42:47
- date last changed
- 2022-01-29 02:32:47
@article{0e766020-02f2-43a1-b7a7-29ae0ed73db9,
abstract = {{BACKGROUND: <br/><br>
Several strategies have been used to reach non-attending women in organized cervical-cancer-screening programs, with varying success. Self-sampling (SS) for HPV is effective for increasing coverage in screening programs, but requires expensive commercial sampling kits. <br/><br>
<br/><br>
OBJECTIVE: <br/><br>
We aimed to evaluate if vaginal SS, without commercial preservatives was adequate for HPV testing. <br/><br>
<br/><br>
STUDY DESIGN: <br/><br>
Women with abnormal cervical smears as determined from the organized screening program were invited to a colposcopy clinic. The 121 women were asked to insert a cotton swab into the vagina and rotate it, put the cotton swab into a sterile cryotube, break the upper part of the stick and put the cap on. Thereafter, the gynaecologist collected a liquid based cytology (LBC) sample. The presence of HPV-types in SS and LBC samples was analysed with PCR and luminex-based typing. <br/><br>
<br/><br>
RESULTS: <br/><br>
High-risk-HPV (hr-HPV) DNA was found in 65 of the tested 108 SS (60%; 95% CI 0.50-0.69), whereas LBC found hr-HPV in 64/108 samples (59%; 95% CI 0.49-0.69). The agreement between sampling with SS and LBC was good, kappa value 0.67 (95% CI; 0.53-0.81). The sensitivity for SS with hr-HPV to find HSIL was 81% (95% CI; 67-95%), specificity 49% (95% CI; 37-60%) and the sensitivity for LBC with hr-HPV to find HSIL was 90% (95% CI 80-100%), specificity53% (95% CI; 42-65%). <br/><br>
<br/><br>
CONCLUSIONS: <br/><br>
This new vaginal self-sampling method detects hr-HPV-infections with similar sensitivity as a cervical smear taken by a gynaecologist. This self-sampling method is cost-effective and well tolerated, and the kit is suitable for regular mail transport.}},
author = {{Darlin, Lotten and Borgfeldt, Christer and Forslund, Ola and Henic, Emir and Dillner, Joakim and Kannisto, Päivi}},
issn = {{1386-6532}},
language = {{eng}},
number = {{1}},
pages = {{52--56}},
publisher = {{Elsevier}},
series = {{Journal of Clinical Virology}},
title = {{Vaginal self-sampling without preservative for human papillomavirus testing shows good sensitivity.}},
url = {{http://dx.doi.org/10.1016/j.jcv.2012.09.002}},
doi = {{10.1016/j.jcv.2012.09.002}},
volume = {{56}},
year = {{2013}},
}