Seed particle-enabled acoustic trapping of bacteria and nanoparticles in continuous flow systems.
(2012) In Lab on a Chip 12(21). p.4296-4304- Abstract
- Acoustic trapping of sub-micron particles can allow enrichment and purification of small-sized and low-abundance microorganisms. In this paper, we investigate the dependency of the ability to capture sub-micron particles on the particle concentration. Based on the findings, it is demonstrated that seed particles can be introduced to acoustic trapping, to enable capture of low-abundance sub-micron particles. Without using seed particles, continuous enrichment of 490 nm polystyrene particles is demonstrated in a rectangular capillary with a locally generated acoustic field at high particle concentrations, i.e. above 1% wt. Trapping of sub-micron particles at significantly lower concentrations was subsequently accomplished by seeding 10-12... (More)
- Acoustic trapping of sub-micron particles can allow enrichment and purification of small-sized and low-abundance microorganisms. In this paper, we investigate the dependency of the ability to capture sub-micron particles on the particle concentration. Based on the findings, it is demonstrated that seed particles can be introduced to acoustic trapping, to enable capture of low-abundance sub-micron particles. Without using seed particles, continuous enrichment of 490 nm polystyrene particles is demonstrated in a rectangular capillary with a locally generated acoustic field at high particle concentrations, i.e. above 1% wt. Trapping of sub-micron particles at significantly lower concentrations was subsequently accomplished by seeding 10-12 micrometer-sized particles in the acoustic trap prior to the sub-micron particle capture. Furthermore, the new seeded-particle-aided acoustic trapping technique was employed for the continuous enrichment of bacteria (E. coli) with a capture efficiency of 95%. Finally, seed particle assisted acoustic trapping and enrichment is demonstrated for polymer-based particles down to 110 nm in diameter. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3124208
- author
- Hammarström, Björn LU ; Laurell, Thomas LU and Nilsson, Johan LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Lab on a Chip
- volume
- 12
- issue
- 21
- pages
- 4296 - 4304
- publisher
- Royal Society of Chemistry
- external identifiers
-
- pmid:22955667
- wos:000310916100013
- scopus:84867287223
- pmid:22955667
- ISSN
- 1473-0189
- DOI
- 10.1039/c2lc40697g
- language
- English
- LU publication?
- yes
- id
- 67bf89d5-5176-4322-a9f6-73f6e4663d4c (old id 3124208)
- date added to LUP
- 2016-04-01 10:42:32
- date last changed
- 2022-04-20 05:24:15
@article{67bf89d5-5176-4322-a9f6-73f6e4663d4c,
abstract = {{Acoustic trapping of sub-micron particles can allow enrichment and purification of small-sized and low-abundance microorganisms. In this paper, we investigate the dependency of the ability to capture sub-micron particles on the particle concentration. Based on the findings, it is demonstrated that seed particles can be introduced to acoustic trapping, to enable capture of low-abundance sub-micron particles. Without using seed particles, continuous enrichment of 490 nm polystyrene particles is demonstrated in a rectangular capillary with a locally generated acoustic field at high particle concentrations, i.e. above 1% wt. Trapping of sub-micron particles at significantly lower concentrations was subsequently accomplished by seeding 10-12 micrometer-sized particles in the acoustic trap prior to the sub-micron particle capture. Furthermore, the new seeded-particle-aided acoustic trapping technique was employed for the continuous enrichment of bacteria (E. coli) with a capture efficiency of 95%. Finally, seed particle assisted acoustic trapping and enrichment is demonstrated for polymer-based particles down to 110 nm in diameter.}},
author = {{Hammarström, Björn and Laurell, Thomas and Nilsson, Johan}},
issn = {{1473-0189}},
language = {{eng}},
number = {{21}},
pages = {{4296--4304}},
publisher = {{Royal Society of Chemistry}},
series = {{Lab on a Chip}},
title = {{Seed particle-enabled acoustic trapping of bacteria and nanoparticles in continuous flow systems.}},
url = {{http://dx.doi.org/10.1039/c2lc40697g}},
doi = {{10.1039/c2lc40697g}},
volume = {{12}},
year = {{2012}},
}