In vitro molecular evolution of antibody genes mimicking receptor revision
Ellmark, Peter LU ; Esteban, O ; Furebring, Christina LU ; Malmborg Hager, Ann-Christin LU and Ohlin, Mats LU
(2002)
In Molecular Immunology
39(5-6).
p.349-356
- Abstract
- Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective... (More)
- Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective pressure. (C) 2002 Elsevier Science Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/325515
- author
- Ellmark, Peter
LU
; Esteban, O
; Furebring, Christina
LU
; Malmborg Hager, Ann-Christin
LU
and Ohlin, Mats
LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- phage display, affinity maturation, antibody, receptor revision, somatic hypermutation
- in
- Molecular Immunology
- volume
- 39
- issue
- 5-6
- pages
- 349 - 356
- publisher
- Pergamon Press Ltd.
- external identifiers
-
- pmid:12220892
- wos:000178578300008
- scopus:0036772983
- ISSN
- 1872-9142
- DOI
- 10.1016/S0161-5890(02)00109-8
- language
- English
- LU publication?
- yes
- id
- e0497862-9d8a-447e-b81d-d9593d16b568 (old id 325515)
- date added to LUP
- 2016-04-01 15:38:55
- date last changed
- 2022-02-27 08:03:06
@article{e0497862-9d8a-447e-b81d-d9593d16b568,
abstract = {{Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective pressure. (C) 2002 Elsevier Science Ltd. All rights reserved.}},
author = {{Ellmark, Peter and Esteban, O and Furebring, Christina and Malmborg Hager, Ann-Christin and Ohlin, Mats}},
issn = {{1872-9142}},
keywords = {{phage display; affinity maturation; antibody; receptor revision; somatic hypermutation}},
language = {{eng}},
number = {{5-6}},
pages = {{349--356}},
publisher = {{Pergamon Press Ltd.}},
series = {{Molecular Immunology}},
title = {{In vitro molecular evolution of antibody genes mimicking receptor revision}},
url = {{http://dx.doi.org/10.1016/S0161-5890(02)00109-8}},
doi = {{10.1016/S0161-5890(02)00109-8}},
volume = {{39}},
year = {{2002}},
}