Polyelectrolyte-coated ion exchangers for cell-resistant expanded bed adsorption
(2002) In Biotechnology Progress 18(4). p.815-820- Abstract
- Adsorption chromatography in expanded beds is a widely used technology for direct capture of target proteins from fermentation broths. However, in many cases this method cannot be applied as a result of the strong tendency of cells or cell debris to interact with the adsorbent beads. To prevent contamination of the expanded bed with the biomass, STREAMLINE DEAE, anion exchanger designed for expanded bed adsorption, was modified with a layer of poly(acrylic acid) (PAA). The shielding layer of polyelectrolyte was attached to the surface of the matrix beads via electrostatic interactions. PAA with a high degree of polymerization was chosen to prevent diffusion of large polymer molecules into the pores of adsorbent. Thus, the shielding layer... (More)
- Adsorption chromatography in expanded beds is a widely used technology for direct capture of target proteins from fermentation broths. However, in many cases this method cannot be applied as a result of the strong tendency of cells or cell debris to interact with the adsorbent beads. To prevent contamination of the expanded bed with the biomass, STREAMLINE DEAE, anion exchanger designed for expanded bed adsorption, was modified with a layer of poly(acrylic acid) (PAA). The shielding layer of polyelectrolyte was attached to the surface of the matrix beads via electrostatic interactions. PAA with a high degree of polymerization was chosen to prevent diffusion of large polymer molecules into the pores of adsorbent. Thus, the shielding layer of PAA was adsorbed, only at the mouth of the pores of STREAMLINE DEAE beads and only marginally decreased the binding capacity of the ion exchanger for bovine serum albumin, the model protein in this study. PAA-coated STREAMLINE DEAE practically did not interact with yeast cells, which other-wise bound,strongly to the native adsorbent at neutral conditions. Cell-resistant PAA-coated anion exchanger was successfully used for isolation of BSA from the model protein mixture containing BSA, lysozyme (positively charged at applied conditions), and yeast cells. The layer of PAA was stable under mild elution conditions, and the modified adsorbent could be used in the repeated purification cycles. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/332155
- author
- Dainiak, Maria LU ; Galaev, Igor LU and Mattiasson, Bo LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Progress
- volume
- 18
- issue
- 4
- pages
- 815 - 820
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000177285200019
- scopus:0036022003
- ISSN
- 1520-6033
- DOI
- 10.1021/bp0202923
- language
- English
- LU publication?
- yes
- id
- 989abb38-0f7a-4ee6-8f60-c6d422042f63 (old id 332155)
- date added to LUP
- 2016-04-01 16:18:36
- date last changed
- 2022-03-30 06:58:51
@article{989abb38-0f7a-4ee6-8f60-c6d422042f63,
abstract = {{Adsorption chromatography in expanded beds is a widely used technology for direct capture of target proteins from fermentation broths. However, in many cases this method cannot be applied as a result of the strong tendency of cells or cell debris to interact with the adsorbent beads. To prevent contamination of the expanded bed with the biomass, STREAMLINE DEAE, anion exchanger designed for expanded bed adsorption, was modified with a layer of poly(acrylic acid) (PAA). The shielding layer of polyelectrolyte was attached to the surface of the matrix beads via electrostatic interactions. PAA with a high degree of polymerization was chosen to prevent diffusion of large polymer molecules into the pores of adsorbent. Thus, the shielding layer of PAA was adsorbed, only at the mouth of the pores of STREAMLINE DEAE beads and only marginally decreased the binding capacity of the ion exchanger for bovine serum albumin, the model protein in this study. PAA-coated STREAMLINE DEAE practically did not interact with yeast cells, which other-wise bound,strongly to the native adsorbent at neutral conditions. Cell-resistant PAA-coated anion exchanger was successfully used for isolation of BSA from the model protein mixture containing BSA, lysozyme (positively charged at applied conditions), and yeast cells. The layer of PAA was stable under mild elution conditions, and the modified adsorbent could be used in the repeated purification cycles.}},
author = {{Dainiak, Maria and Galaev, Igor and Mattiasson, Bo}},
issn = {{1520-6033}},
language = {{eng}},
number = {{4}},
pages = {{815--820}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Biotechnology Progress}},
title = {{Polyelectrolyte-coated ion exchangers for cell-resistant expanded bed adsorption}},
url = {{http://dx.doi.org/10.1021/bp0202923}},
doi = {{10.1021/bp0202923}},
volume = {{18}},
year = {{2002}},
}