Microcultivation of anaerobic bacteria single cells entrapped in alginate microbeads.
(2013) In Biotechnology Letters 35(3). p.397-405- Abstract
- Alginate microbeads, produced by emulsion/internal gelation, were studied for the entrapment and microcultivation of microbial cells with biotechnological potential. An anaerobic consortium which was selected for its capacity to degrade complex carbohydrates, and a pure culture of cellulose degrading bacteria were used for entrapment studies. Optimization of conditions for the formation of spherical alginate microbeads in sizes between 20 and 80 μm were examined. The best conditions were achieved by combining rapeseed methyl ester as oil phase and stirring at 100 rpm using a rotation impeller. Calcium alginate microbeads produced under these conditions were shown to present morphological stability, with large pores in the internal matrix... (More)
- Alginate microbeads, produced by emulsion/internal gelation, were studied for the entrapment and microcultivation of microbial cells with biotechnological potential. An anaerobic consortium which was selected for its capacity to degrade complex carbohydrates, and a pure culture of cellulose degrading bacteria were used for entrapment studies. Optimization of conditions for the formation of spherical alginate microbeads in sizes between 20 and 80 μm were examined. The best conditions were achieved by combining rapeseed methyl ester as oil phase and stirring at 100 rpm using a rotation impeller. Calcium alginate microbeads produced under these conditions were shown to present morphological stability, with large pores in the internal matrix that favours microcolony development. Finally, single cells were observed inside the beads after the entrapment procedure and microcolony formation was confirmed after cultivation in cellobiose. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3347417
- author
- Aragao, Rosa LU ; Alvarez Aliaga, Teresa LU and Mattiasson, Bo LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Microcultivation, Single cell, Alginate, Anaerobic bacteria
- in
- Biotechnology Letters
- volume
- 35
- issue
- 3
- pages
- 397 - 405
- publisher
- Springer
- external identifiers
-
- wos:000314788400014
- pmid:23224821
- scopus:84873999308
- pmid:23224821
- ISSN
- 1573-6776
- DOI
- 10.1007/s10529-012-1094-1
- language
- English
- LU publication?
- yes
- id
- 469910e7-2c22-43bf-bb8a-bbb46c6ea6ba (old id 3347417)
- date added to LUP
- 2016-04-01 13:14:40
- date last changed
- 2022-04-06 03:29:18
@article{469910e7-2c22-43bf-bb8a-bbb46c6ea6ba,
abstract = {{Alginate microbeads, produced by emulsion/internal gelation, were studied for the entrapment and microcultivation of microbial cells with biotechnological potential. An anaerobic consortium which was selected for its capacity to degrade complex carbohydrates, and a pure culture of cellulose degrading bacteria were used for entrapment studies. Optimization of conditions for the formation of spherical alginate microbeads in sizes between 20 and 80 μm were examined. The best conditions were achieved by combining rapeseed methyl ester as oil phase and stirring at 100 rpm using a rotation impeller. Calcium alginate microbeads produced under these conditions were shown to present morphological stability, with large pores in the internal matrix that favours microcolony development. Finally, single cells were observed inside the beads after the entrapment procedure and microcolony formation was confirmed after cultivation in cellobiose.}},
author = {{Aragao, Rosa and Alvarez Aliaga, Teresa and Mattiasson, Bo}},
issn = {{1573-6776}},
keywords = {{Microcultivation; Single cell; Alginate; Anaerobic bacteria}},
language = {{eng}},
number = {{3}},
pages = {{397--405}},
publisher = {{Springer}},
series = {{Biotechnology Letters}},
title = {{Microcultivation of anaerobic bacteria single cells entrapped in alginate microbeads.}},
url = {{http://dx.doi.org/10.1007/s10529-012-1094-1}},
doi = {{10.1007/s10529-012-1094-1}},
volume = {{35}},
year = {{2013}},
}