Fetal hepatic progenitors support long-term expansion of hematopoietic stem cells
(2013) In Experimental Hematology 41(5). p.479-490- Abstract
- We have developed a coculture system that establishes DLK+ fetal hepatic progenitors as the authentic supportive cells for expansion of hematopoietic stem (HSCs) and progenitor cells. In 1-week cultures supplemented with serum and supportive cytokines, both cocultured DLK+ fetal hepatic progenitors and their conditioned medium supported rapid expansion of hematopoietic progenitors and a small increase in HSC numbers. In 2- and 3-week cultures DLK+ cells, but not their conditioned medium, continuously and significantly (>20-fold) expanded both hematopoietic stem and progenitor cells. Physical contact between HSCs and DLK+ cells was crucial to maintaining this long-term expansion. Similar HSC expansion (approximately sevenfold) was... (More)
- We have developed a coculture system that establishes DLK+ fetal hepatic progenitors as the authentic supportive cells for expansion of hematopoietic stem (HSCs) and progenitor cells. In 1-week cultures supplemented with serum and supportive cytokines, both cocultured DLK+ fetal hepatic progenitors and their conditioned medium supported rapid expansion of hematopoietic progenitors and a small increase in HSC numbers. In 2- and 3-week cultures DLK+ cells, but not their conditioned medium, continuously and significantly (>20-fold) expanded both hematopoietic stem and progenitor cells. Physical contact between HSCs and DLK+ cells was crucial to maintaining this long-term expansion. Similar HSC expansion (approximately sevenfold) was achieved in cocultures using a serum-free, low cytokine- containing medium. In contrast, DLK- cells are incapable of expanding hematopoietic cells, demonstrating that hepatic progenitors are the principle supportive cells for HSC expansion in the fetal liver. (C) 2013 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3815461
- author
- Chou, Song ; Flygare, Johan LU and Lodish, Harvey F.
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Experimental Hematology
- volume
- 41
- issue
- 5
- pages
- 479 - 490
- publisher
- Elsevier
- external identifiers
-
- wos:000318666700007
- scopus:84876864454
- ISSN
- 1873-2399
- DOI
- 10.1016/j.exphem.2013.02.003
- language
- English
- LU publication?
- yes
- id
- cef7c985-b9b7-42d0-a643-df9ead282a8c (old id 3815461)
- date added to LUP
- 2016-04-01 09:55:38
- date last changed
- 2022-04-12 00:13:03
@article{cef7c985-b9b7-42d0-a643-df9ead282a8c,
abstract = {{We have developed a coculture system that establishes DLK+ fetal hepatic progenitors as the authentic supportive cells for expansion of hematopoietic stem (HSCs) and progenitor cells. In 1-week cultures supplemented with serum and supportive cytokines, both cocultured DLK+ fetal hepatic progenitors and their conditioned medium supported rapid expansion of hematopoietic progenitors and a small increase in HSC numbers. In 2- and 3-week cultures DLK+ cells, but not their conditioned medium, continuously and significantly (>20-fold) expanded both hematopoietic stem and progenitor cells. Physical contact between HSCs and DLK+ cells was crucial to maintaining this long-term expansion. Similar HSC expansion (approximately sevenfold) was achieved in cocultures using a serum-free, low cytokine- containing medium. In contrast, DLK- cells are incapable of expanding hematopoietic cells, demonstrating that hepatic progenitors are the principle supportive cells for HSC expansion in the fetal liver. (C) 2013 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.}},
author = {{Chou, Song and Flygare, Johan and Lodish, Harvey F.}},
issn = {{1873-2399}},
language = {{eng}},
number = {{5}},
pages = {{479--490}},
publisher = {{Elsevier}},
series = {{Experimental Hematology}},
title = {{Fetal hepatic progenitors support long-term expansion of hematopoietic stem cells}},
url = {{http://dx.doi.org/10.1016/j.exphem.2013.02.003}},
doi = {{10.1016/j.exphem.2013.02.003}},
volume = {{41}},
year = {{2013}},
}