The Kruppel-associated box repressor domain can trigger de novo promoter methylation during mouse early embryogenesis
Wiznerowicz, Maciej ; Jakobsson, Johan LU
; Szulc, Jolanta
; Liao, Shunyao
; Quazzola, Alexandra
; Beermann, Friedrich
; Aebischer, Patrick
and Trono, Didier
(2007)
In Journal of Biological Chemistry
282(47).
p.41-34535
- Abstract
The Krüppel-associated box (KRAB) domain is a transcriptional repression module responsible for the DNA binding-dependent gene silencing activity of hundreds of vertebrate zinc finger proteins. We previously exploited KRAB-mediated repression within the context of a tet repressor-KRAB fusion protein and of lentiviral vectors to create a method of external gene control. We demonstrated that with this system transcriptional silencing was fully reversible in cell culture as well as in vivo. Here we reveal that, in sharp contrast, KRAB-mediated repression results in irreversible gene silencing through promoter DNA methylation if it acts during the first few days of mouse development.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/38911131-0ef1-4618-aaa9-0d37a9cf52cb
- author
- Wiznerowicz, Maciej
; Jakobsson, Johan
LU
; Szulc, Jolanta
; Liao, Shunyao
; Quazzola, Alexandra
; Beermann, Friedrich
; Aebischer, Patrick
and Trono, Didier
- publishing date
- 2007-11-23
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Carrier Proteins, DNA Methylation, Embryonic Development, Gene Silencing, Lentivirus, Mice, Nuclear Proteins, Promoter Regions, Genetic, Protein Structure, Tertiary, Repressor Proteins, Transcription, Genetic, Journal Article, Research Support, Non-U.S. Gov't
- in
- Journal of Biological Chemistry
- volume
- 282
- issue
- 47
- pages
- 7 pages
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- pmid:17893143
- scopus:36348930084
- ISSN
- 0021-9258
- DOI
- 10.1074/jbc.M705898200
- language
- English
- LU publication?
- no
- id
- 38911131-0ef1-4618-aaa9-0d37a9cf52cb
- date added to LUP
- 2016-11-23 14:42:26
- date last changed
- 2024-04-19 13:18:03
@article{38911131-0ef1-4618-aaa9-0d37a9cf52cb,
abstract = {{<p>The Krüppel-associated box (KRAB) domain is a transcriptional repression module responsible for the DNA binding-dependent gene silencing activity of hundreds of vertebrate zinc finger proteins. We previously exploited KRAB-mediated repression within the context of a tet repressor-KRAB fusion protein and of lentiviral vectors to create a method of external gene control. We demonstrated that with this system transcriptional silencing was fully reversible in cell culture as well as in vivo. Here we reveal that, in sharp contrast, KRAB-mediated repression results in irreversible gene silencing through promoter DNA methylation if it acts during the first few days of mouse development.</p>}},
author = {{Wiznerowicz, Maciej and Jakobsson, Johan and Szulc, Jolanta and Liao, Shunyao and Quazzola, Alexandra and Beermann, Friedrich and Aebischer, Patrick and Trono, Didier}},
issn = {{0021-9258}},
keywords = {{Animals; Carrier Proteins; DNA Methylation; Embryonic Development; Gene Silencing; Lentivirus; Mice; Nuclear Proteins; Promoter Regions, Genetic; Protein Structure, Tertiary; Repressor Proteins; Transcription, Genetic; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
month = {{11}},
number = {{47}},
pages = {{41--34535}},
publisher = {{American Society for Biochemistry and Molecular Biology}},
series = {{Journal of Biological Chemistry}},
title = {{The Kruppel-associated box repressor domain can trigger de novo promoter methylation during mouse early embryogenesis}},
url = {{http://dx.doi.org/10.1074/jbc.M705898200}},
doi = {{10.1074/jbc.M705898200}},
volume = {{282}},
year = {{2007}},
}