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Simultaneous saccharification and fermentation of steam-pretreated spruce to ethanol

Bollok, M ; Reczey, K and Zacchi, Guido LU (2000) In Applied Biochemistry and Biotechnology 84-6. p.69-80
Abstract
Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of substrate, at a cellulase loading of 37 filter paper units/g of cellulose, and a beta-glucosidase loading of 38 IU/ g of cellulose. The detoxification of the substrate and the lack of pH control in the experiments increased the final ethanol concentration. The final ethanol yield was 15% lower compared to SSF with Saccharomyces cerevisiae at 37 degrees C, owing to the cessation of ethanol fermentation after the first 10 h.
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
simultaneous saccharification and fermentation, Kluyveromyces marxianus, ethanol fermentation, steam-pretreated spruce
in
Applied Biochemistry and Biotechnology
volume
84-6
pages
69 - 80
publisher
Humana Press
external identifiers
  • wos:000087211500007
  • scopus:0034039256
ISSN
1559-0291
DOI
10.1385/ABAB:84-86:1-9:69
language
English
LU publication?
yes
id
9957ef82-bfa1-42d7-af83-34b1db8d8ed0 (old id 3911366)
date added to LUP
2016-04-01 12:20:44
date last changed
2023-09-02 04:25:30
@article{9957ef82-bfa1-42d7-af83-34b1db8d8ed0,
  abstract     = {{Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of substrate, at a cellulase loading of 37 filter paper units/g of cellulose, and a beta-glucosidase loading of 38 IU/ g of cellulose. The detoxification of the substrate and the lack of pH control in the experiments increased the final ethanol concentration. The final ethanol yield was 15% lower compared to SSF with Saccharomyces cerevisiae at 37 degrees C, owing to the cessation of ethanol fermentation after the first 10 h.}},
  author       = {{Bollok, M and Reczey, K and Zacchi, Guido}},
  issn         = {{1559-0291}},
  keywords     = {{simultaneous saccharification and fermentation; Kluyveromyces marxianus; ethanol fermentation; steam-pretreated spruce}},
  language     = {{eng}},
  pages        = {{69--80}},
  publisher    = {{Humana Press}},
  series       = {{Applied Biochemistry and Biotechnology}},
  title        = {{Simultaneous saccharification and fermentation of steam-pretreated spruce to ethanol}},
  url          = {{http://dx.doi.org/10.1385/ABAB:84-86:1-9:69}},
  doi          = {{10.1385/ABAB:84-86:1-9:69}},
  volume       = {{84-6}},
  year         = {{2000}},
}