Batch adjustment by reference alignment (BARA) : Improved prediction performance in biological test sets with batch effects
(2019) In PLoS ONE 14(2).- Abstract
Many biological data acquisition platforms suffer from inadvertent inclusion of biologically irrelevant variance in analyzed data, collectively termed batch effects. Batch effects can lead to difficulties in downstream analysis by lowering the power to detect biologically interesting differences and can in certain instances lead to false discoveries. They are especially troublesome in predictive modelling where samples in training sets and test sets are often completely correlated with batches. In this article, we present BARA, a normalization method for adjusting batch effects in predictive modelling. BARA utilizes a few reference samples to adjust for batch effects in a compressed data space spanned by the training set. We evaluate... (More)
Many biological data acquisition platforms suffer from inadvertent inclusion of biologically irrelevant variance in analyzed data, collectively termed batch effects. Batch effects can lead to difficulties in downstream analysis by lowering the power to detect biologically interesting differences and can in certain instances lead to false discoveries. They are especially troublesome in predictive modelling where samples in training sets and test sets are often completely correlated with batches. In this article, we present BARA, a normalization method for adjusting batch effects in predictive modelling. BARA utilizes a few reference samples to adjust for batch effects in a compressed data space spanned by the training set. We evaluate BARA using a collection of publicly available datasets and three different prediction models, and compare its performance to already existing methods developed for similar purposes. The results show that data normalized with BARA generates high and consistent prediction performances. Further, they suggest that BARA produces reliable performances independent of the examined classifiers. We therefore conclude that BARA has great potential to facilitate the development of predictive assays where test sets and training sets are correlated with batch.
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- author
- Gradin, Robin LU ; Lindstedt, Malin LU and Johansson, Henrik LU
- organization
- publishing date
- 2019-02-22
- type
- Contribution to journal
- publication status
- published
- subject
- in
- PLoS ONE
- volume
- 14
- issue
- 2
- article number
- e0212669
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- pmid:30794641
- scopus:85061975258
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0212669
- language
- English
- LU publication?
- yes
- id
- 3f673bb9-450b-4808-9b68-16da6a9fa06b
- date added to LUP
- 2019-03-06 13:00:26
- date last changed
- 2024-02-14 19:00:10
@article{3f673bb9-450b-4808-9b68-16da6a9fa06b, abstract = {{<p>Many biological data acquisition platforms suffer from inadvertent inclusion of biologically irrelevant variance in analyzed data, collectively termed batch effects. Batch effects can lead to difficulties in downstream analysis by lowering the power to detect biologically interesting differences and can in certain instances lead to false discoveries. They are especially troublesome in predictive modelling where samples in training sets and test sets are often completely correlated with batches. In this article, we present BARA, a normalization method for adjusting batch effects in predictive modelling. BARA utilizes a few reference samples to adjust for batch effects in a compressed data space spanned by the training set. We evaluate BARA using a collection of publicly available datasets and three different prediction models, and compare its performance to already existing methods developed for similar purposes. The results show that data normalized with BARA generates high and consistent prediction performances. Further, they suggest that BARA produces reliable performances independent of the examined classifiers. We therefore conclude that BARA has great potential to facilitate the development of predictive assays where test sets and training sets are correlated with batch.</p>}}, author = {{Gradin, Robin and Lindstedt, Malin and Johansson, Henrik}}, issn = {{1932-6203}}, language = {{eng}}, month = {{02}}, number = {{2}}, publisher = {{Public Library of Science (PLoS)}}, series = {{PLoS ONE}}, title = {{Batch adjustment by reference alignment (BARA) : Improved prediction performance in biological test sets with batch effects}}, url = {{http://dx.doi.org/10.1371/journal.pone.0212669}}, doi = {{10.1371/journal.pone.0212669}}, volume = {{14}}, year = {{2019}}, }