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A novel model of dormancy for bone metastatic breast cancer cells

Marlow, Rebecca ; Honeth, Gabriella LU ; Lombardi, Sara ; Cariati, Massimiliano ; Hessey, Sonya ; Pipili, Aikaterini ; Mariotti, Veronica ; Buchupalli, Bharath ; Foster, Katie and Bonnet, Dominique , et al. (2013) In Cancer Research 73(23). p.99-6886
Abstract

Mortality of patients with breast cancer is due overwhelmingly to metastatic spread of the disease. Although dissemination is an early event in breast cancer, extended periods of cancer cell dormancy can result in long latency of metastasis development. Deciphering the mechanisms underlying cancer cell dormancy and subsequent growth at the metastatic site would facilitate development of strategies to interfere with these processes. A challenge in this undertaking has been the lack of models for cancer cell dormancy. We have established novel experimental systems that model the bone microenvironment of the breast cancer metastatic niche. These systems are based on 3D cocultures of breast cancer cells with cell types predominant in bone... (More)

Mortality of patients with breast cancer is due overwhelmingly to metastatic spread of the disease. Although dissemination is an early event in breast cancer, extended periods of cancer cell dormancy can result in long latency of metastasis development. Deciphering the mechanisms underlying cancer cell dormancy and subsequent growth at the metastatic site would facilitate development of strategies to interfere with these processes. A challenge in this undertaking has been the lack of models for cancer cell dormancy. We have established novel experimental systems that model the bone microenvironment of the breast cancer metastatic niche. These systems are based on 3D cocultures of breast cancer cells with cell types predominant in bone marrow. We identified conditions in which cancer cells are dormant and conditions in which they proliferate. Dormant cancer cells were able to proliferate upon transfer into supportive microenvironment or upon manipulation of signaling pathways that control dormancy. These experimental systems will be instrumental for metastasis studies, particularly the study of cellular dormancy.

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publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Bone Marrow Cells, Bone Neoplasms, Breast Neoplasms, Cell Cycle Checkpoints, Cells, Cultured, Female, Human Umbilical Vein Endothelial Cells, Humans, MCF-7 Cells, Mice, Mice, Inbred NOD, Mice, SCID, Models, Biological, Neoplastic Stem Cells, Stem Cell Niche, Stromal Cells, Tumor Microenvironment, Journal Article, Research Support, Non-U.S. Gov't
in
Cancer Research
volume
73
issue
23
pages
14 pages
publisher
American Association for Cancer Research Inc.
external identifiers
  • pmid:24145351
  • scopus:84890259230
ISSN
1538-7445
DOI
10.1158/0008-5472.CAN-13-0991
language
English
LU publication?
no
id
41e52b3a-d611-48a9-8c2f-b691f18dc202
date added to LUP
2016-11-25 16:50:24
date last changed
2024-04-19 14:28:49
@article{41e52b3a-d611-48a9-8c2f-b691f18dc202,
  abstract     = {{<p>Mortality of patients with breast cancer is due overwhelmingly to metastatic spread of the disease. Although dissemination is an early event in breast cancer, extended periods of cancer cell dormancy can result in long latency of metastasis development. Deciphering the mechanisms underlying cancer cell dormancy and subsequent growth at the metastatic site would facilitate development of strategies to interfere with these processes. A challenge in this undertaking has been the lack of models for cancer cell dormancy. We have established novel experimental systems that model the bone microenvironment of the breast cancer metastatic niche. These systems are based on 3D cocultures of breast cancer cells with cell types predominant in bone marrow. We identified conditions in which cancer cells are dormant and conditions in which they proliferate. Dormant cancer cells were able to proliferate upon transfer into supportive microenvironment or upon manipulation of signaling pathways that control dormancy. These experimental systems will be instrumental for metastasis studies, particularly the study of cellular dormancy.</p>}},
  author       = {{Marlow, Rebecca and Honeth, Gabriella and Lombardi, Sara and Cariati, Massimiliano and Hessey, Sonya and Pipili, Aikaterini and Mariotti, Veronica and Buchupalli, Bharath and Foster, Katie and Bonnet, Dominique and Grigoriadis, Agamemnon and Rameshwar, Pranela and Purushotham, Anand and Tutt, Andrew and Dontu, Gabriela}},
  issn         = {{1538-7445}},
  keywords     = {{Animals; Bone Marrow Cells; Bone Neoplasms; Breast Neoplasms; Cell Cycle Checkpoints; Cells, Cultured; Female; Human Umbilical Vein Endothelial Cells; Humans; MCF-7 Cells; Mice; Mice, Inbred NOD; Mice, SCID; Models, Biological; Neoplastic Stem Cells; Stem Cell Niche; Stromal Cells; Tumor Microenvironment; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{23}},
  pages        = {{99--6886}},
  publisher    = {{American Association for Cancer Research Inc.}},
  series       = {{Cancer Research}},
  title        = {{A novel model of dormancy for bone metastatic breast cancer cells}},
  url          = {{http://dx.doi.org/10.1158/0008-5472.CAN-13-0991}},
  doi          = {{10.1158/0008-5472.CAN-13-0991}},
  volume       = {{73}},
  year         = {{2013}},
}